Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OLL83_RS02355 Genome accession   NZ_CP110338
Coordinates   501805..503511 (-) Length   568 a.a.
NCBI ID   WP_156020398.1    Uniprot ID   -
Organism   Shewanella algae strain B2215466     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496805..508511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OLL83_RS02325 (OLL83_000465) mutT 497399..497797 (-) 399 WP_208171869.1 8-oxo-dGTP diphosphatase MutT -
  OLL83_RS02330 (OLL83_000466) yacG 497797..498009 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  OLL83_RS02335 (OLL83_000467) zapD 498054..498794 (-) 741 WP_028779562.1 cell division protein ZapD -
  OLL83_RS02340 (OLL83_000468) coaE 498791..499402 (-) 612 WP_054744929.1 dephospho-CoA kinase -
  OLL83_RS02345 (OLL83_000469) pilD 499403..500317 (-) 915 WP_144191483.1 A24 family peptidase Machinery gene
  OLL83_RS02350 (OLL83_000470) pilC 500387..501652 (-) 1266 WP_054744931.1 type II secretion system F family protein Machinery gene
  OLL83_RS02355 (OLL83_000471) pilB 501805..503511 (-) 1707 WP_156020398.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OLL83_RS02360 (OLL83_000472) - 503521..503934 (-) 414 WP_054744933.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OLL83_RS02365 (OLL83_000473) nadC 504792..505652 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OLL83_RS02370 (OLL83_000474) ampD 505844..506389 (+) 546 WP_145839838.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OLL83_RS02375 (OLL83_000475) ampE 506443..507294 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  OLL83_RS02380 (OLL83_000476) pdhR 507570..508322 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63149.28 Da        Isoelectric Point: 5.3439

>NTDB_id=754087 OLL83_RS02355 WP_156020398.1 501805..503511(-) (pilB) [Shewanella algae strain B2215466]
MPTSGLHLGLSTLFIRKGLLTENQLTETIRHSQKNKQTLVTTLVASNLLSARTIAELCYEEYGTPLLDLTEFDLNSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLNKALEKVLEEDLSSLDLDGLDEES
LAGIEVTDTDKRQQEDSESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFT
QQQIDAGFTPYKPCGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=754087 OLL83_RS02355 WP_156020398.1 501805..503511(-) (pilB) [Shewanella algae strain B2215466]
ATGCCAACATCCGGCTTACACCTAGGTCTGTCGACTCTTTTTATCCGTAAAGGTCTACTGACGGAAAATCAGTTAACCGA
AACGATAAGACATTCCCAGAAAAATAAACAAACACTGGTCACCACTCTGGTGGCCAGTAATCTTTTATCCGCTCGCACCA
TCGCCGAACTCTGCTATGAAGAATACGGCACCCCGCTGCTGGATCTCACTGAGTTTGATTTAAACAGTATCCCGGAAGAA
TTTCTCAATAAAAAGCTGATCGACAAGCACAGATGCCTGCCGTTATTTAAGCGTGGCAACCGCCTCTATATCGCCACCTC
AGACCCCACCAATATAGCCGCCCTGGAAGACTTCCAATTTAGCGCTGGCCTGCATGCCGAAGCAATACTGGTAGAGGAAG
ATAAGCTCAATAAAGCACTGGAAAAGGTGCTCGAAGAAGACCTGAGTTCACTGGACCTGGATGGCCTCGATGAGGAATCC
CTCGCAGGTATCGAGGTCACAGATACCGACAAACGTCAACAGGAAGATAGTGAAAGTAAAGATGACGCCCCCATCGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCTTACGAAAAACGCTACC
GCATCCGTTTTCGTATCGACGGTATTCTCCACGAGGTGTCCGAGCCACCCATTTCTCTCTCGGGCCGTATCTCAGCCCGC
TTGAAGGTGATGTCCAAACTGGATATCGCCGAGCGCCGGGTCCCTCAGGATGGCCGCATCAAAATGAGGCTGTCGCGCAA
CAAGAGTATCGATTTTCGGGTCAGTACCCTGCCCACCCTCTGGGGTGAAAAGATAGTGATGCGGATCCTCGACTCCTCTT
CCGCACAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAAATGCTGGCAAGGCCCCAG
GGGATGATTCTGGTGACAGGCCCTACCGGCTCTGGTAAAACCGTTTCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAACGCAATATCAGCACAGCCGAAGACCCGGTGGAGATCAACCTGGAAGGGGTTAACCAGGTGCATATCAACCCCAAGG
CCGGACTGACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCGGCACAAACCGGCCACTTGGTACTCTCGACCCTGCACACCAACTCATCGGC
TGAGACTCTCACCCGCCTGCTTAACATGGGGGTTCCCGGTTATAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTATGCCCCGAATGCCGCCAACCGGAAGATATTCCCGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAGACGCAGGCTTTACCCCATACAAGCCCTGCGGCTGCGACCTCTGTTCCGGTGGTTATAAGGGAAGGGT
CGGTATCTATGAGGTGATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCG
CCACGGCCAAACAACAAGGGATGCGCGACCTGCGCCTTTCAGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAGATAAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.537

98.768

0.588
  pilB Acinetobacter baumannii D1279779

60.223

94.718

0.57
  pilB Legionella pneumophila strain ERS1305867

55.477

99.648

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

52.734

99.824

0.526
  pilB Vibrio cholerae strain A1552

53.455

96.831

0.518
  pilB Vibrio campbellii strain DS40M4

51.155

99.12

0.507
  pilF Neisseria gonorrhoeae MS11

47.965

99.472

0.477
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.517

97.359

0.375
  pilF Thermus thermophilus HB27

37.411

99.296

0.371