Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OLL86_RS06400 Genome accession   NZ_CP110225
Coordinates   1368121..1369530 (-) Length   469 a.a.
NCBI ID   WP_264779689.1    Uniprot ID   -
Organism   Gallibacterium anatis strain IMT49310     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1363121..1374530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OLL86_RS06365 (OLL86_06365) - 1363405..1364064 (+) 660 WP_013746615.1 formate dehydrogenase subunit gamma -
  OLL86_RS06370 (OLL86_06370) fdhE 1364066..1364989 (+) 924 WP_021461527.1 formate dehydrogenase accessory protein FdhE -
  OLL86_RS06375 (OLL86_06375) - 1365055..1365327 (-) 273 WP_264779685.1 GNAT family N-acetyltransferase -
  OLL86_RS06380 (OLL86_06380) yacG 1365413..1365631 (-) 219 WP_018345915.1 DNA gyrase inhibitor YacG -
  OLL86_RS06385 (OLL86_06385) coaE 1365621..1366253 (-) 633 WP_264779686.1 dephospho-CoA kinase -
  OLL86_RS06390 (OLL86_06390) - 1366268..1366900 (-) 633 WP_264779687.1 prepilin peptidase -
  OLL86_RS06395 (OLL86_06395) - 1366897..1368105 (-) 1209 WP_264779688.1 type II secretion system F family protein -
  OLL86_RS06400 (OLL86_06400) pilB 1368121..1369530 (-) 1410 WP_264779689.1 GspE/PulE family protein Machinery gene
  OLL86_RS06405 (OLL86_06405) pilA 1369547..1370008 (-) 462 WP_039148357.1 prepilin peptidase-dependent pilin Machinery gene
  OLL86_RS06410 (OLL86_06410) ampD 1370113..1370667 (+) 555 WP_264779690.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OLL86_RS06415 (OLL86_06415) - 1370754..1371089 (+) 336 WP_013746603.1 hypothetical protein -
  OLL86_RS06420 (OLL86_06420) - 1371198..1372592 (+) 1395 WP_264779691.1 chloride channel protein -
  OLL86_RS06425 (OLL86_06425) dusA 1372573..1373553 (+) 981 WP_264779692.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52823.27 Da        Isoelectric Point: 5.4667

>NTDB_id=753191 OLL86_RS06400 WP_264779689.1 1368121..1369530(-) (pilB) [Gallibacterium anatis strain IMT49310]
MGYCVKALDSEQLFEISELQWQKNQQQYELLLRYLAVPLQEQAQQLWLAVESFDNLAACETFAFLSGKQIEPVAIGQTAL
KQLLQQLQPGTATNQVQESQLHSYQQNDIVAKSDEIAEVDAQEPIIQLFNQLLESGLQYSVSDIHLEPLRQAFQIRFRID
GVLQRHQVLTLSIGQRLNSRLKLLAKLDINELRLPQDGRFRFKTAFGETLDIRLSSLPTQYGEKIVLRLQPNTPVFDDFT
ELGMTNEQAVLFRQALSQPQGLILVTGPTGSGKSLTLYSGLHFLNQESRHILTAEDPIELQLNGIIQTQANAAIGLTFAT
LLRSFLRQDPDVIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTAIGAISRLQQLGIAQHELENSLLLVVAQRLVRKRC
TLCQGDKSQQCDCVDGYRGRTGVYQLLHYYQQQIQMDYPTLLAAAQLKLQQRITTTAEVERVFGKTENS

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=753191 OLL86_RS06400 WP_264779689.1 1368121..1369530(-) (pilB) [Gallibacterium anatis strain IMT49310]
ATGGGCTATTGTGTAAAGGCATTGGATTCGGAGCAGCTGTTTGAAATTTCAGAATTGCAATGGCAGAAGAATCAACAGCA
ATATGAACTGTTATTGCGTTATTTGGCGGTACCGTTGCAGGAACAGGCGCAACAGTTATGGTTAGCGGTTGAGTCTTTCG
ATAATTTAGCCGCTTGCGAGACCTTTGCTTTTCTTTCCGGTAAACAGATTGAGCCGGTTGCGATCGGACAAACTGCACTG
AAACAGTTATTACAGCAGTTGCAACCCGGAACGGCGACAAATCAAGTGCAAGAATCACAACTGCATAGTTATCAGCAAAA
TGATATTGTCGCAAAATCGGATGAAATTGCGGAAGTTGATGCACAAGAGCCGATTATTCAACTATTCAATCAATTATTGG
AATCAGGTTTGCAATATTCCGTTTCTGATATTCATCTTGAGCCGTTACGTCAGGCGTTCCAGATTCGTTTTAGGATTGAC
GGCGTATTACAACGGCATCAGGTGCTGACATTGTCAATAGGTCAACGCTTAAACTCACGCCTAAAATTGTTGGCAAAACT
GGATATTAACGAATTACGCTTGCCGCAGGATGGTCGTTTTCGTTTTAAAACCGCATTCGGTGAAACATTAGATATTCGGT
TATCTTCTCTACCGACACAATATGGTGAAAAAATCGTATTACGATTGCAACCTAACACACCGGTGTTTGATGATTTTACC
GAACTGGGAATGACAAACGAGCAGGCTGTGCTGTTCCGGCAAGCGTTATCTCAACCGCAAGGATTAATCTTGGTTACCGG
ACCGACCGGAAGTGGTAAAAGTTTGACATTATATAGCGGTTTACATTTTTTAAATCAGGAAAGTCGCCACATTCTTACGG
CGGAAGATCCGATAGAACTGCAATTAAACGGTATTATTCAGACCCAAGCAAATGCCGCAATCGGGTTAACTTTTGCGACA
TTATTGCGGAGTTTTTTACGCCAAGATCCGGATGTCATTATGGTTGGGGAAATTCGAGATCAGGAAACGGCAGAGATAGC
ATTACGCGCGGCACAGACAGGACATTTAGTGTTATCGACACTGCATACCAATACGGCAATCGGTGCAATTTCCCGATTAC
AACAGTTGGGAATAGCACAACATGAATTGGAAAATTCATTATTGCTGGTGGTGGCACAACGCTTAGTTCGCAAACGCTGT
ACCTTATGCCAAGGCGATAAATCCCAACAATGCGATTGTGTTGACGGTTATCGCGGACGAACAGGGGTTTATCAACTGCT
ACATTACTATCAACAACAAATTCAAATGGATTATCCGACATTATTGGCGGCAGCACAGCTGAAATTACAGCAACGGATCA
CGACAACAGCAGAGGTTGAACGCGTCTTCGGCAAGACGGAGAACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(131-398)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-91)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

59.211

97.228

0.576
  pilB Haemophilus influenzae 86-028NP

59.211

97.228

0.576
  pilB Glaesserella parasuis strain SC1401

53.632

99.787

0.535
  pilB Vibrio parahaemolyticus RIMD 2210633

38.677

100

0.412
  pilB Vibrio cholerae strain A1552

39.834

100

0.409
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.407
  pilB/pilB1 Synechocystis sp. PCC 6803

36.276

100

0.403
  pilB Acinetobacter baylyi ADP1

39.503

94.456

0.373
  pilB Legionella pneumophila strain ERS1305867

35.95

100

0.371
  pilB Acinetobacter baumannii D1279779

39.13

93.177

0.365