Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OK026_RS17105 Genome accession   NZ_CP109905
Coordinates   3721206..3722912 (+) Length   568 a.a.
NCBI ID   WP_114153189.1    Uniprot ID   -
Organism   Chromobacterium haemolyticum strain UGAL515B_03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3716206..3727912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK026_RS17085 (OK026_17110) - 3716798..3717619 (+) 822 WP_114153191.1 inner membrane protein YpjD -
  OK026_RS17090 (OK026_17115) - 3717654..3718034 (+) 381 WP_043637938.1 helix-hairpin-helix domain-containing protein -
  OK026_RS17095 (OK026_17120) - 3718006..3719532 (-) 1527 WP_317646237.1 deoxyribodipyrimidine photo-lyase -
  OK026_RS17100 (OK026_17125) - 3719836..3720984 (+) 1149 WP_194427604.1 porin -
  OK026_RS17105 (OK026_17130) pilF 3721206..3722912 (+) 1707 WP_114153189.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OK026_RS17110 (OK026_17135) pilC 3723125..3724348 (+) 1224 WP_194427605.1 type II secretion system F family protein Machinery gene
  OK026_RS17115 (OK026_17140) pilD 3724345..3725214 (+) 870 WP_194427606.1 A24 family peptidase Machinery gene
  OK026_RS17120 (OK026_17145) coaE 3725204..3725815 (+) 612 WP_081555553.1 dephospho-CoA kinase -
  OK026_RS17125 (OK026_17150) zapD 3725854..3726612 (+) 759 WP_019100891.1 cell division protein ZapD -
  OK026_RS17130 (OK026_17155) yacG 3726650..3726838 (+) 189 WP_043591348.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62208.79 Da        Isoelectric Point: 5.4415

>NTDB_id=750502 OK026_RS17105 WP_114153189.1 3721206..3722912(+) (pilF) [Chromobacterium haemolyticum strain UGAL515B_03]
MEASAGISGLGRALVQNNMLLQQDADAILRQSQASHISFVEQLIQSKKMTAQDVAMFASKTFGYPLLDMSAIDLDQLPKG
VVDDELVASRRLLPLLKRGNKLYVGTSDPTQTSAYHAITFKTGMTVEIVVVEDDKLGKMLGKYSEDATAAIKALENEDFG
DLEFADPDAPTDAGPQPDVDDAPVVKFIHKVLLDAINGGASDIHFEPYEKFYRIRYRVDGVLKEISQPPLVLKEKIASRI
KVISKLDISEKRVPQDGRMKLVISKARAIDFRVSTLPTLYGEKIVMRILDSSAANLDIEQLGFEPEQKRLLLDAIARPYG
MVLVTGPTGSGKTVSLYTCLNLLNKPDINIATAEDPVEINLPGINQVNVNERAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNNAPATLTRMLNMGVAPFNIASSVLLIMAQRLARRLCAHCKKPAEIPEEALLRAGFKQ
EELDGSWKPYAPVGCEECRNTGYRGRTGIYEVMPITDAMTRLIMQNGTAIDIADLARKEGMVDLRHAGLLKVKAGLTSLT
EIEAVTNE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=750502 OK026_RS17105 WP_114153189.1 3721206..3722912(+) (pilF) [Chromobacterium haemolyticum strain UGAL515B_03]
ATGGAAGCTTCGGCTGGCATTTCCGGCTTGGGTCGGGCCTTGGTGCAAAACAATATGCTGTTGCAGCAGGATGCGGACGC
AATCCTGCGCCAGTCTCAGGCATCGCACATCAGCTTTGTCGAGCAGCTGATTCAGAGCAAGAAGATGACGGCGCAGGATG
TGGCGATGTTCGCGTCCAAGACCTTTGGTTACCCCTTGCTGGACATGTCGGCCATTGATCTTGATCAGTTGCCCAAGGGC
GTGGTTGATGACGAGTTGGTCGCCAGCCGGCGCTTACTGCCCCTGCTCAAGCGTGGCAACAAGCTGTATGTAGGCACTTC
CGATCCCACCCAGACCTCCGCCTATCACGCCATCACCTTCAAGACTGGGATGACGGTGGAAATCGTGGTGGTGGAGGATG
ATAAGCTGGGCAAGATGCTCGGCAAGTACAGCGAGGATGCCACCGCCGCGATCAAGGCCTTGGAGAACGAGGATTTTGGC
GATCTTGAATTCGCCGATCCGGATGCGCCGACCGATGCCGGCCCACAGCCGGATGTCGATGATGCGCCTGTGGTCAAGTT
CATCCATAAGGTGTTGTTGGATGCGATCAATGGCGGCGCGTCGGATATTCATTTCGAGCCCTATGAGAAGTTTTATCGCA
TCCGCTACCGGGTAGACGGGGTGTTGAAGGAGATTTCGCAGCCGCCCCTGGTATTGAAGGAAAAAATCGCCTCCCGGATC
AAGGTGATTTCCAAACTGGATATTTCGGAGAAGCGAGTGCCGCAGGATGGCCGGATGAAGCTGGTCATCTCCAAGGCGCG
GGCAATCGACTTCCGGGTCAGCACCTTGCCCACCTTGTACGGCGAGAAGATCGTCATGCGTATTCTGGACTCGTCGGCGG
CGAATCTGGATATCGAGCAGTTGGGCTTTGAACCGGAGCAGAAACGCCTACTGCTGGACGCGATTGCGCGGCCCTACGGC
ATGGTGCTGGTCACCGGACCTACCGGTAGCGGTAAAACGGTGTCCTTGTACACCTGCCTCAATCTGTTGAACAAGCCGGA
TATCAATATCGCCACCGCCGAAGACCCTGTGGAAATCAACTTGCCGGGCATCAATCAGGTCAACGTCAACGAGCGTGCCG
GGCTGACCTTCGCTTCGGCCTTGAAGGCCTTCCTGCGTCAGGATCCCGACGTGATCATGGTGGGTGAGATCCGGGATTTC
GAAACGGCGGACATCGCGATCAAGGCGGCTCAGACCGGCCATATGGTGTTTTCCACTCTACACACCAATAACGCGCCGGC
GACTTTGACCCGGATGCTGAATATGGGGGTGGCGCCGTTCAACATCGCCAGCTCGGTGTTGCTGATCATGGCGCAACGTT
TGGCGCGCCGTTTGTGCGCGCATTGCAAGAAACCGGCGGAGATTCCGGAGGAGGCATTGCTGCGGGCGGGATTCAAGCAA
GAGGAGTTGGATGGAAGCTGGAAGCCCTATGCGCCGGTGGGTTGCGAGGAGTGTCGCAACACCGGCTACCGTGGCCGTAC
CGGTATTTACGAAGTGATGCCGATTACCGATGCGATGACGCGTTTGATCATGCAAAACGGTACCGCCATCGATATCGCCG
ACCTGGCGCGCAAGGAGGGCATGGTGGACTTGCGCCATGCCGGTTTGCTGAAGGTGAAGGCGGGGTTGACGTCGTTGACG
GAGATTGAAGCGGTGACTAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.46

99.472

0.562
  pilB Acinetobacter baumannii D1279779

53.713

100

0.548
  pilB Acinetobacter baylyi ADP1

53.641

99.12

0.532
  pilB Legionella pneumophila strain ERS1305867

51.579

100

0.518
  pilB Vibrio cholerae strain A1552

49.281

97.887

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

46.975

98.944

0.465
  pilB Vibrio campbellii strain DS40M4

45.826

99.12

0.454
  pilF Thermus thermophilus HB27

37.943

99.296

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.544

98.944

0.371