Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PALA25_RS23650 Genome accession   NZ_CP109834
Coordinates   5098702..5100294 (+) Length   530 a.a.
NCBI ID   WP_003123394.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA25     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5093702..5105294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA25_RS23630 (PALA25_04714) pgeF 5095452..5096180 (-) 729 WP_003112833.1 peptidoglycan editing factor PgeF -
  PALA25_RS23635 (PALA25_04715) rluD 5096177..5097139 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PALA25_RS23640 (PALA25_04716) - 5097285..5098310 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  PALA25_RS23645 (PALA25_04717) - 5098467..5098712 (+) 246 WP_003102591.1 PP0621 family protein -
  PALA25_RS23650 (PALA25_04718) pilS 5098702..5100294 (+) 1593 WP_003123394.1 two-component system sensor histidine kinase PilS Regulator
  PALA25_RS23655 (PALA25_04719) pilR 5100309..5101646 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  PALA25_RS23660 (PALA25_04720) thiO 5101692..5102786 (-) 1095 WP_023122775.1 glycine oxidase ThiO -
  PALA25_RS23665 (PALA25_04721) - 5102926..5103426 (+) 501 WP_016562346.1 GspH/FimT family pseudopilin -
  PALA25_RS23670 (PALA25_04722) - 5103549..5104037 (+) 489 WP_003141552.1 GspH/FimT family pseudopilin -
  PALA25_RS23675 (PALA25_04723) pilV 5104047..5104571 (+) 525 WP_003116261.1 type IV pilus modification protein PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58999.18 Da        Isoelectric Point: 6.6886

>NTDB_id=749813 PALA25_RS23650 WP_003123394.1 5098702..5100294(+) (pilS) [Pseudomonas aeruginosa strain PALA25]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEIIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=749813 PALA25_RS23650 WP_003123394.1 5098702..5100294(+) (pilS) [Pseudomonas aeruginosa strain PALA25]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTGGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCCAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAATCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.811

100

0.998