Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GL2_RS11905 Genome accession   NZ_AP019807
Coordinates   2733911..2735623 (-) Length   570 a.a.
NCBI ID   WP_143730857.1    Uniprot ID   A0A510ITU8
Organism   Microbulbifer sp. GL-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2728911..2740623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GL2_RS11875 (GL2_24120) argJ 2728958..2730160 (+) 1203 WP_143730851.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  GL2_RS11880 (GL2_24130) mutT 2730171..2730572 (+) 402 WP_143730852.1 8-oxo-dGTP diphosphatase MutT -
  GL2_RS11885 (GL2_24140) yacG 2730636..2730854 (-) 219 WP_143730853.1 DNA gyrase inhibitor YacG -
  GL2_RS11890 (GL2_24150) coaE 2730929..2731534 (-) 606 WP_143730854.1 dephospho-CoA kinase -
  GL2_RS11895 (GL2_24160) pilD 2731649..2732533 (-) 885 WP_143730855.1 A24 family peptidase Machinery gene
  GL2_RS11900 (GL2_24170) pilC 2732540..2733754 (-) 1215 WP_143730856.1 type II secretion system F family protein Machinery gene
  GL2_RS11905 (GL2_24180) pilB 2733911..2735623 (-) 1713 WP_143730857.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GL2_RS22215 (GL2_24190) - 2736010..2736426 (+) 417 WP_172621132.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GL2_RS11915 - 2736513..2737376 (+) 864 WP_197736446.1 sterol desaturase family protein -
  GL2_RS11920 (GL2_24200) - 2737441..2740614 (-) 3174 WP_143730859.1 error-prone DNA polymerase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 61826.46 Da        Isoelectric Point: 5.3390

>NTDB_id=74220 GL2_RS11905 WP_143730857.1 2733911..2735623(-) (pilB) [Microbulbifer sp. GL-2]
MTSSPLSGLAKRLVADQILDETTAASASKAARRAGQSFAQHAVEAKLVKSRELANIASLAFGTPLFDLGCYNFEMIPKGV
VDEKLISKHFALPLYKRGNRLFVAVADPTNLAGLDEINFNTGLSTDAVLVEADKLGKAIESYLSDGGDISAGLDGIDDED
LDSLNVGGSESESDDESEPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKSYRVRFRTDGVLHEVSKPPIQLATRLSA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEDEQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGINQVNVSSKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCSECKKAATLPNEVLEAEGF
SAVSIPNSEWTIYQPVGCEHCSKGYKGRVGVYEVVRITDSISRIIMEGGNSIQIADQARKDGFNNLRTSALRKVVMGITS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=74220 GL2_RS11905 WP_143730857.1 2733911..2735623(-) (pilB) [Microbulbifer sp. GL-2]
ATGACTAGCTCCCCCCTTAGTGGCCTGGCAAAACGCCTGGTAGCAGACCAAATCCTCGATGAAACCACTGCCGCCTCCGC
CTCAAAGGCAGCCCGCCGGGCAGGTCAGTCTTTTGCCCAGCATGCCGTTGAAGCCAAGCTGGTAAAAAGTCGCGAACTTG
CCAATATTGCCTCCCTGGCCTTTGGCACACCGTTATTTGACCTCGGTTGTTATAACTTCGAAATGATTCCCAAGGGAGTG
GTTGACGAGAAGTTGATCAGCAAGCATTTCGCCCTCCCTCTATATAAGCGTGGAAATCGCCTCTTTGTTGCGGTAGCAGA
CCCAACCAACCTGGCAGGGTTAGACGAGATTAACTTCAACACCGGCCTCAGCACAGACGCTGTTTTGGTAGAAGCCGACA
AGCTTGGAAAGGCGATTGAAAGCTACCTCTCCGACGGCGGAGACATTAGTGCCGGCCTAGATGGTATCGATGATGAGGAT
CTGGACTCGCTCAATGTGGGGGGGAGCGAATCTGAATCCGACGATGAGAGCGAGCCGGGAGGAGATGAAGCCCCGGTTGT
TCGCTTTGTCAATAAAGTTCTACTTGACGCCATTCGCACCGGAGCCTCCGACATCCACTTCGAGCCTTATGAAAAATCCT
ACCGGGTTCGCTTCCGCACCGACGGTGTACTGCATGAAGTTTCCAAGCCACCCATTCAGCTGGCCACAAGGCTATCTGCC
CGTTTAAAGGTGATGTCGAAAATGGACATCTCAGAACGGAGAGTACCTCAAGACGGCCGGATTAAAATGAAGCTGTCCAA
AACCAAGGCTATCGACTTCCGGGTAAACAGCCTGCCGACACTTTGGGGGGAAAAGATTGTTCTGCGAATTCTCGACCCCT
CTTCAGCAAAACTGGGAATCGATGCGCTCGGTTATGAGGACGAGCAAAAAAAGATCTATATGGATGCCCTGGCCCAACCC
CAGGGTATGATCCTGGTTACCGGGCCAACAGGCTCAGGCAAAACAGTTTCCCTCTATACCGGTCTCAACATACTCAATAC
TGCTGAACGGAATATATCCACCGCAGAAGACCCGGTGGAGATCAATCTGGAAGGGATTAATCAGGTCAACGTCTCTTCCA
AAGTAGGCCTTAATTTTGCCGAGGCCCTGCGTTCATTCCTGCGGCAAGACCCTGATATCGTGATGGTGGGGGAGATTCGG
GACCTGGAGACCGCGGAGATTGCCATTAAAGCGGCGCAGACTGGGCACTTAGTACTCTCAACATTACACACAAACTCCGC
CCCGGAAACCCTCACACGCCTGATGAACATGGGAGTGCCTACTTTTAATATCGCCACTTCTGTCAGTGTTATTATCGCCC
AGCGCTTGGCAAGGCGTTTATGTAGCGAGTGCAAAAAGGCCGCTACTCTTCCAAATGAAGTGCTGGAAGCAGAAGGCTTT
TCTGCAGTAAGCATCCCCAATAGCGAATGGACAATCTACCAGCCGGTTGGCTGCGAACACTGCTCCAAGGGCTACAAGGG
GCGCGTAGGCGTGTATGAAGTGGTTCGCATCACTGATAGCATCTCCAGAATTATAATGGAGGGCGGCAACTCGATTCAGA
TTGCCGACCAGGCTCGCAAAGATGGCTTTAACAACCTAAGAACCTCAGCACTGCGCAAAGTGGTGATGGGTATTACCAGT
TTGGAAGAAGCCAACCGAGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510ITU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.228

100

0.612
  pilB Acinetobacter baylyi ADP1

58.947

100

0.589
  pilB Legionella pneumophila strain ERS1305867

54.657

99.825

0.546
  pilB Vibrio parahaemolyticus RIMD 2210633

51.767

99.298

0.514
  pilB Vibrio campbellii strain DS40M4

51.858

99.123

0.514
  pilB Vibrio cholerae strain A1552

52.468

95.965

0.504
  pilF Neisseria gonorrhoeae MS11

47.619

99.474

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

38.699

100

0.418

Multiple sequence alignment