Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GL2_RS11895 Genome accession   NZ_AP019807
Coordinates   2731649..2732533 (-) Length   294 a.a.
NCBI ID   WP_143730855.1    Uniprot ID   A0A510ITU1
Organism   Microbulbifer sp. GL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2726649..2737533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GL2_RS11875 (GL2_24120) argJ 2728958..2730160 (+) 1203 WP_143730851.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  GL2_RS11880 (GL2_24130) mutT 2730171..2730572 (+) 402 WP_143730852.1 8-oxo-dGTP diphosphatase MutT -
  GL2_RS11885 (GL2_24140) yacG 2730636..2730854 (-) 219 WP_143730853.1 DNA gyrase inhibitor YacG -
  GL2_RS11890 (GL2_24150) coaE 2730929..2731534 (-) 606 WP_143730854.1 dephospho-CoA kinase -
  GL2_RS11895 (GL2_24160) pilD 2731649..2732533 (-) 885 WP_143730855.1 A24 family peptidase Machinery gene
  GL2_RS11900 (GL2_24170) pilC 2732540..2733754 (-) 1215 WP_143730856.1 type II secretion system F family protein Machinery gene
  GL2_RS11905 (GL2_24180) pilB 2733911..2735623 (-) 1713 WP_143730857.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GL2_RS22215 (GL2_24190) - 2736010..2736426 (+) 417 WP_172621132.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GL2_RS11915 - 2736513..2737376 (+) 864 WP_197736446.1 sterol desaturase family protein -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32454.45 Da        Isoelectric Point: 7.6599

>NTDB_id=74218 GL2_RS11895 WP_143730855.1 2731649..2732533(-) (pilD) [Microbulbifer sp. GL-2]
MIELISNYPALLLSSAFIFGLLIGSFLNVVILRLPVMMEREYKRDFYSYFDTKPDSASQEELSKPFNLVLPHSHCPKCKT
EIKPWQNIPLISYLFLRGRCSSCGTAISKRYPMVELVTGILTAVVVWQLGFTWQALAGCAFTWVLIALTGIDFDKQLLPD
NLSLPLLWGGLLINLWGTFVPLQDAVIGAIAGYLSLWLVFHIFKLVTGKEGMGAGDFKILAAIGAWFGWQMLPLVILLSA
AVGAIAGITWSLAVGRDHRLPIAFGPYLAGAGWIAMLWGEQIVSWYMSFSGLGS

Nucleotide


Download         Length: 885 bp        

>NTDB_id=74218 GL2_RS11895 WP_143730855.1 2731649..2732533(-) (pilD) [Microbulbifer sp. GL-2]
ATGATAGAACTTATCTCTAACTATCCTGCGCTGCTATTGAGCAGCGCTTTTATTTTTGGCCTGTTAATAGGTAGCTTCCT
CAATGTGGTAATCCTGCGCCTGCCAGTTATGATGGAGCGGGAGTACAAGCGGGATTTTTACAGTTACTTCGACACAAAAC
CTGATTCCGCATCGCAGGAAGAGTTGAGTAAACCTTTCAATCTGGTATTGCCCCACTCCCATTGCCCCAAGTGCAAAACT
GAGATCAAACCCTGGCAAAATATTCCGTTAATCAGCTACTTATTTTTGCGTGGCAGGTGCAGCTCCTGTGGTACAGCCAT
TTCCAAGCGTTACCCCATGGTGGAACTGGTCACCGGTATTCTCACCGCAGTTGTGGTGTGGCAACTGGGCTTTACTTGGC
AGGCATTGGCTGGATGTGCGTTTACCTGGGTCTTGATCGCCCTCACTGGTATCGATTTTGACAAGCAGCTATTACCAGAC
AACCTGTCTCTGCCACTGCTCTGGGGTGGGTTGCTGATCAATTTGTGGGGCACTTTTGTTCCATTGCAGGATGCGGTTAT
CGGGGCGATCGCTGGCTATCTGTCGCTGTGGCTGGTATTCCATATCTTTAAGCTGGTTACTGGCAAAGAGGGGATGGGAG
CTGGGGACTTCAAGATCCTCGCCGCTATTGGGGCCTGGTTTGGCTGGCAGATGCTGCCGTTGGTGATTCTTTTGTCTGCC
GCCGTGGGCGCTATTGCCGGTATCACCTGGAGCCTGGCTGTGGGGCGGGACCACAGACTACCAATTGCCTTTGGGCCCTA
TTTGGCCGGAGCTGGTTGGATCGCCATGTTGTGGGGTGAGCAGATAGTAAGCTGGTATATGAGCTTCTCTGGACTTGGCA
GCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(140-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510ITU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

50.347

97.959

0.493
  pilD Acinetobacter nosocomialis M2

49.47

96.259

0.476
  pilD Acinetobacter baumannii D1279779

49.117

96.259

0.473
  pilD Vibrio campbellii strain DS40M4

48.264

97.959

0.473
  pilD Neisseria gonorrhoeae MS11

49.638

93.878

0.466

Multiple sequence alignment