Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilC   Type   Machinery gene
Locus tag   OF401_RS05385 Genome accession   NZ_CP107717
Coordinates   1282499..1283761 (-) Length   420 a.a.
NCBI ID   WP_057671824.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain XY1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1237246..1293152 1282499..1283761 within 0


Gene organization within MGE regions


Location: 1237246..1293152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OF401_RS05185 (OF401_05185) - 1237246..1239126 (+) 1881 WP_019237331.1 integrase arm-type DNA-binding domain-containing protein -
  OF401_RS05190 (OF401_05190) - 1239311..1239730 (+) 420 WP_019237332.1 hypothetical protein -
  OF401_RS05195 (OF401_05195) - 1239763..1240764 (+) 1002 WP_011038253.1 XVIPCD domain-containing protein -
  OF401_RS05200 (OF401_05200) - 1240835..1241485 (-) 651 WP_011038252.1 LysR substrate-binding domain-containing protein -
  OF401_RS05205 (OF401_05205) - 1241588..1241881 (+) 294 WP_011038251.1 helix-turn-helix transcriptional regulator -
  OF401_RS05210 (OF401_05210) - 1241909..1242793 (-) 885 Protein_1005 LysR family transcriptional regulator -
  OF401_RS05215 (OF401_05215) - 1243546..1247646 (-) 4101 WP_081020747.1 HigA family addiction module antitoxin -
  OF401_RS05220 (OF401_05220) - 1248127..1251390 (-) 3264 WP_057671623.1 ATP-binding protein -
  OF401_RS05225 (OF401_05225) - 1251481..1251612 (+) 132 Protein_1008 IS5/IS1182 family transposase -
  OF401_RS05230 (OF401_05230) - 1252581..1254119 (+) 1539 WP_011038243.1 MASE1 domain-containing protein -
  OF401_RS05235 (OF401_05235) - 1255141..1255788 (+) 648 WP_016848927.1 hypothetical protein -
  OF401_RS05240 (OF401_05240) - 1255820..1256959 (+) 1140 WP_017117946.1 type IV secretion system protein -
  OF401_RS05245 (OF401_05245) - 1257037..1257576 (+) 540 WP_225443876.1 DUF4189 domain-containing protein -
  OF401_RS05250 (OF401_05250) - 1257974..1258504 (+) 531 WP_011038239.1 hypothetical protein -
  OF401_RS05255 (OF401_05255) - 1258501..1259160 (+) 660 WP_057671625.1 hypothetical protein -
  OF401_RS05260 (OF401_05260) - 1259157..1260539 (+) 1383 WP_057671628.1 DUF6792 domain-containing protein -
  OF401_RS05265 (OF401_05265) - 1260643..1260992 (+) 350 Protein_1016 type IV secretion system protein -
  OF401_RS05270 (OF401_05270) - 1261579..1262723 (+) 1145 WP_087942063.1 IS3 family transposase -
  OF401_RS05275 (OF401_05275) radC 1263272..1263751 (+) 480 WP_075286085.1 DNA repair protein RadC -
  OF401_RS05280 (OF401_05280) - 1263822..1264013 (+) 192 WP_221261829.1 hypothetical protein -
  OF401_RS22205 - 1265791..1266216 (+) 426 Protein_1020 XVIPCD domain-containing protein -
  OF401_RS05290 (OF401_05290) - 1266244..1266498 (-) 255 WP_057671639.1 hypothetical protein -
  OF401_RS05295 (OF401_05295) - 1266765..1268360 (+) 1596 WP_192012501.1 MobA/MobL family protein -
  OF401_RS05300 (OF401_05300) - 1268433..1268801 (-) 369 WP_225443877.1 transcriptional regulator -
  OF401_RS05305 (OF401_05305) - 1268809..1269090 (-) 282 WP_011348355.1 DUF6516 family protein -
  OF401_RS05310 (OF401_05310) - 1269156..1269497 (-) 342 WP_081020748.1 hypothetical protein -
  OF401_RS05315 (OF401_05315) - 1270223..1270573 (+) 351 WP_225443878.1 hypothetical protein -
  OF401_RS05320 (OF401_05320) - 1270873..1271103 (-) 231 WP_157382680.1 hypothetical protein -
  OF401_RS05325 (OF401_05325) - 1271167..1271313 (+) 147 WP_040940792.1 hypothetical protein -
  OF401_RS05330 (OF401_05330) - 1271498..1271893 (+) 396 WP_042594933.1 hypothetical protein -
  OF401_RS05335 (OF401_05335) - 1271984..1272376 (+) 393 WP_012437593.1 H-NS family nucleoid-associated regulatory protein -
  OF401_RS05340 (OF401_05340) glgX 1272911..1275040 (-) 2130 WP_057671651.1 glycogen debranching protein GlgX -
  OF401_RS05345 (OF401_05345) rimK 1275534..1276409 (+) 876 WP_016945000.1 30S ribosomal protein S6--L-glutamate ligase -
  OF401_RS05350 (OF401_05350) - 1277059..1277736 (+) 678 WP_003490678.1 response regulator transcription factor -
  OF401_RS05355 (OF401_05355) - 1277729..1279063 (+) 1335 WP_057671653.1 HAMP domain-containing sensor histidine kinase -
  OF401_RS05360 (OF401_05360) - 1279206..1279697 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  OF401_RS05365 (OF401_05365) - 1279694..1279984 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  OF401_RS05370 (OF401_05370) - 1280059..1280460 (-) 402 WP_040940795.1 SymE family type I addiction module toxin -
  OF401_RS05375 (OF401_05375) coaE 1280992..1281615 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  OF401_RS05380 (OF401_05380) - 1281629..1282492 (-) 864 WP_057671659.1 A24 family peptidase -
  OF401_RS05385 (OF401_05385) pilC 1282499..1283761 (-) 1263 WP_057671824.1 type II secretion system F family protein Machinery gene
  OF401_RS05390 (OF401_05390) pilA 1284103..1284531 (+) 429 WP_057671660.1 pilin Machinery gene
  OF401_RS05395 (OF401_05395) pilB 1284667..1286397 (+) 1731 WP_057671826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OF401_RS05405 (OF401_05405) pilR 1286816..1288210 (-) 1395 WP_264252082.1 sigma-54 dependent transcriptional regulator Regulator
  OF401_RS05410 (OF401_05410) - 1288419..1290029 (-) 1611 WP_057671664.1 HAMP domain-containing sensor histidine kinase -
  OF401_RS05415 (OF401_05415) sucC 1290263..1291432 (+) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  OF401_RS05420 (OF401_05420) sucD 1291457..1292332 (+) 876 WP_057671668.1 succinate--CoA ligase subunit alpha -
  OF401_RS05425 (OF401_05425) - 1292436..1292846 (+) 411 WP_029217117.1 DNA-binding protein -
  OF401_RS05430 (OF401_05430) - 1292850..1293152 (+) 303 WP_011038207.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence


Protein


Download         Length: 420 a.a.        Molecular weight: 46232.76 Da        Isoelectric Point: 10.2689

>NTDB_id=741580 OF401_RS05385 WP_057671824.1 1282499..1283761(-) (pilC) [Xanthomonas campestris pv. campestris strain XY1-1]
MSVVRNAIKKQVVDRSTGQQLQLFLWEGTDKRGIKMKGEQTARNMNMLRAELRRQGINPSVVKPKPKPLFGAAGKKITPK
DIAFFSRQMATMMKSGVPIVSSLEIIGEGHKNPRMKKMVGQVRTDIEGGSSLYESISRHPVQFDELYRNLVRAGEGAGVL
ETVLDTVATYKENIEALKGKIKKALFYPAMVIAVALIVSAILLIFVVPQFEEVFKGFGAELPAFTQMIVGASRFMVSYWW
IMLFVVAGSIIGFIFAYKRSPSMQHAMDRLILRVPVIGQIMHNSSIARFARTTAVTFKAGVPLVEALGIVAGATGNRVYE
DAVLRMRDDVSVGYPVNMAMKQVNLFPHMVIQMTAIGEEAGALDTMLFKVAEYFEQEVNNAVDALSSLLEPMIMVFIGVV
VGGMVIGMYLPIFKLGAVVG

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=741580 OF401_RS05385 WP_057671824.1 1282499..1283761(-) (pilC) [Xanthomonas campestris pv. campestris strain XY1-1]
ATGTCTGTCGTACGCAATGCTATTAAGAAGCAAGTAGTCGACCGCAGTACTGGTCAACAGCTACAGCTGTTTCTTTGGGA
AGGAACCGATAAGCGCGGAATCAAGATGAAAGGCGAGCAGACTGCGCGCAACATGAATATGTTGCGCGCGGAACTTCGTC
GTCAAGGTATCAATCCGTCGGTAGTCAAACCCAAACCCAAGCCGCTGTTCGGTGCTGCAGGCAAGAAAATTACGCCAAAA
GACATTGCATTTTTCAGTCGCCAGATGGCGACTATGATGAAATCAGGGGTGCCGATTGTAAGCTCGCTTGAGATCATCGG
TGAGGGCCACAAAAATCCACGCATGAAAAAGATGGTGGGCCAAGTGAGAACGGATATCGAGGGCGGCTCATCTCTTTATG
AATCAATCAGCAGACATCCCGTGCAGTTTGATGAGCTCTACCGAAACCTCGTCCGAGCAGGTGAAGGTGCAGGCGTTCTA
GAGACAGTGCTAGATACAGTTGCCACTTACAAAGAAAACATAGAGGCCCTTAAGGGAAAGATTAAGAAGGCCCTGTTTTA
TCCAGCCATGGTGATTGCAGTCGCCCTTATTGTCAGCGCCATCCTGCTCATTTTTGTGGTCCCTCAATTCGAAGAGGTTT
TTAAGGGTTTCGGCGCCGAGTTACCCGCCTTCACCCAAATGATTGTAGGAGCATCACGCTTCATGGTGAGCTACTGGTGG
ATCATGCTGTTCGTTGTAGCAGGCTCCATCATTGGCTTCATCTTTGCTTATAAACGCTCACCAAGCATGCAGCATGCCAT
GGATAGGCTGATATTGAGAGTGCCTGTTATCGGGCAAATCATGCATAACAGCTCGATTGCGCGCTTCGCACGCACGACTG
CGGTTACCTTTAAAGCCGGAGTCCCATTAGTGGAAGCATTAGGCATTGTTGCTGGCGCCACCGGCAACCGCGTCTACGAA
GATGCGGTACTCCGTATGCGCGACGATGTATCTGTTGGCTACCCGGTCAACATGGCGATGAAACAGGTAAACCTGTTCCC
ACACATGGTTATTCAAATGACTGCGATTGGCGAAGAAGCGGGCGCTCTTGATACCATGCTCTTCAAGGTGGCGGAATACT
TCGAGCAAGAGGTAAACAATGCAGTAGATGCACTTAGCAGCCTGCTCGAACCGATGATTATGGTCTTTATTGGCGTCGTC
GTAGGCGGTATGGTCATCGGCATGTATCTTCCGATCTTCAAACTCGGCGCAGTGGTTGGATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.408

94.524

0.514

  pilC Legionella pneumophila strain ERS1305867

52.882

95

0.502

  pilC Acinetobacter baylyi ADP1

51.244

95.714

0.49

  pilC Acinetobacter baumannii D1279779

49.628

95.952

0.476

  pilG Neisseria gonorrhoeae MS11

44.638

95.476

0.426

  pilG Neisseria meningitidis 44/76-A

44.14

95.476

0.421

  pilC Vibrio campbellii strain DS40M4

40.786

96.905

0.395

  pilC Vibrio cholerae strain A1552

40.75

95.238

0.388