Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OEG79_RS17420 Genome accession   NZ_CP107549
Coordinates   3648709..3650415 (+) Length   568 a.a.
NCBI ID   WP_264146200.1    Uniprot ID   -
Organism   Pseudomonas sp. Z8(2022)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3643709..3655415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEG79_RS17400 (OEG79_17400) nadC 3646264..3647112 (+) 849 WP_264146198.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OEG79_RS17405 (OEG79_17405) tfpZ 3647145..3647882 (-) 738 WP_264146199.1 TfpX/TfpZ family type IV pilin accessory protein -
  OEG79_RS17410 (OEG79_17410) - 3647995..3648483 (-) 489 WP_318840823.1 pilin -
  OEG79_RS17420 (OEG79_17420) pilB 3648709..3650415 (+) 1707 WP_264146200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OEG79_RS17425 (OEG79_17425) pilC 3650418..3651635 (+) 1218 WP_264146201.1 type II secretion system F family protein Machinery gene
  OEG79_RS17430 (OEG79_17430) pilD 3651636..3652505 (+) 870 WP_264146202.1 prepilin peptidase Machinery gene
  OEG79_RS17435 (OEG79_17435) coaE 3652674..3653282 (+) 609 WP_264146203.1 dephospho-CoA kinase -
  OEG79_RS17440 (OEG79_17440) yacG 3653279..3653473 (+) 195 WP_264146204.1 DNA gyrase inhibitor YacG -
  OEG79_RS17445 (OEG79_17445) - 3653510..3653725 (-) 216 WP_264146205.1 hypothetical protein -
  OEG79_RS17450 (OEG79_17450) - 3653831..3654520 (-) 690 WP_264146206.1 energy-coupling factor ABC transporter permease -
  OEG79_RS17455 (OEG79_17455) - 3654720..3655349 (+) 630 WP_264146207.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62493.43 Da        Isoelectric Point: 5.3390

>NTDB_id=740589 OEG79_RS17420 WP_264146200.1 3648709..3650415(+) (pilB) [Pseudomonas sp. Z8(2022)]
MNENISLSGLAKQMVLAELMDEKAAQQAQQQAQRNKLSLVTYLVQNKLVKSRALAELAAEQFGVAFFDLNAIDKDGQPRD
LISEKLVRQHRVLPLWRRGNKLFVGLSDPTNHQAVTDVQFSTGLNTEAILVEDDKLGDAIDKFFDSANSGMEDLADVDLD
GLDVEAVDDDRQTGDGGGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGLLHEVARPPIQLAPRISA
RLKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYLAALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIR
DLDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNSCKKEVNVPRETLLAEGF
PEDKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIAAQMRKDGFNDLRTSALLKAMQGVTSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=740589 OEG79_RS17420 WP_264146200.1 3648709..3650415(+) (pilB) [Pseudomonas sp. Z8(2022)]
ATGAACGAGAACATCTCCCTGTCTGGCCTGGCCAAACAGATGGTGCTGGCCGAACTAATGGACGAGAAAGCCGCCCAACA
GGCCCAGCAACAAGCGCAACGAAACAAGCTTTCGCTAGTGACTTACCTGGTACAGAACAAGTTGGTTAAAAGTCGTGCCC
TGGCAGAGCTAGCCGCCGAACAGTTTGGCGTCGCCTTTTTCGACCTGAACGCTATAGACAAGGACGGTCAACCCCGCGAC
CTCATCAGCGAGAAATTGGTACGCCAGCATCGGGTGCTCCCATTATGGCGCCGGGGCAACAAGCTGTTCGTCGGACTTTC
TGATCCGACCAATCACCAGGCCGTGACGGATGTGCAGTTCAGCACCGGCCTCAATACCGAGGCAATTCTGGTCGAGGACG
ACAAACTGGGCGATGCCATCGACAAGTTCTTCGACTCGGCCAATAGCGGCATGGAAGATCTGGCCGATGTGGACCTGGAC
GGACTGGACGTCGAAGCAGTAGATGACGATAGGCAAACCGGTGACGGCGGGGGGGGCGATGCCGATGACGCGCCAGTCGT
ACGCTTCGTCAACAAAATGCTGCTCGACGCCATCAAAGGGGGCTCATCCGACCTGCACTTCGAACCCTACGAGAAGGCCT
ACCGGGTACGCTTTCGTACCGACGGCCTGCTACACGAAGTGGCGCGCCCGCCAATTCAGCTTGCTCCGCGCATTTCCGCA
CGCCTAAAGGTGATGGCCGCTCTGGATATTTCCGAGCGACGCAAGCCGCAGGATGGCCGGATCAAGATGAAAATATCCAA
GACCAAAGCCATCGACTTTCGCGTCAACACCCTGCCCACACTGTGGGGTGAGAAGATCGTGATGCGGATTCTCGATCCGA
CCAGCGCACAGATGGGCATCGACGCTCTGGGTTACGAGGAGGAGCAGAAAGAGCTGTATCTGGCAGCCTTGAAGCAACCT
CAGGGCATGATTCTGGTCACCGGACCCACCGGCTCAGGCAAAACGGTTTCGCTCTATACCGGCCTGAATATCCTCAACAC
TCCGGATGTGAATATCTCCACGGCCGAAGACCCCGTGGAGATCAACCTGGAAGGCATCAACCAGGTTAACGTCAATCCCA
AGCAGGGCATGGATTTCACCCAGGCACTACGCGCCTTCCTGCGCCAGGATCCGGATATCATCATGGTCGGTGAGATTCGA
GATCTGGACACCGCCTCCATCGCGGTCAAGGCCGCGCAAACCGGCCACATGGTGATGTCCACCCTGCACACCAACAGCGC
CGCGGAAACCCTGACCCGCCTGCGCAACATGGGTGTGCCATCCTTCAACATCGCCACCTCCGTCAACCTGATCATTGCTC
AGCGCCTGGCGCGCAAGCTGTGCAACAGCTGCAAGAAGGAAGTTAACGTGCCGCGTGAAACCCTGCTGGCCGAGGGCTTC
CCTGAGGACAAGATCGGTACTTTCAAAATCTATGGCCCGGTAGGTTGCGATAACTGCAAAGGCGGTTACAAAGGCCGTGT
CGGTATTTATGAAGTGGTTAAAAACACGCCGAACCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCG
CACAGATGCGTAAAGACGGCTTTAATGACCTACGCACCTCCGCTTTACTCAAGGCCATGCAAGGGGTTACCAGCCTTGAA
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.156

99.296

0.577
  pilB Acinetobacter baumannii D1279779

58.053

99.472

0.577
  pilB Legionella pneumophila strain ERS1305867

54.912

100

0.551
  pilB Vibrio cholerae strain A1552

49.113

99.296

0.488
  pilF Neisseria gonorrhoeae MS11

48.582

99.296

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.36

96.303

0.475
  pilB Vibrio campbellii strain DS40M4

48.54

96.479

0.468
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.848

92.782

0.37
  pilF Thermus thermophilus HB27

36.926

99.648

0.368