Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5938_RS10190 Genome accession   NZ_CP107257
Coordinates   2186629..2188329 (-) Length   566 a.a.
NCBI ID   WP_263901898.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2019CK-00034     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2181629..2193329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5938_RS10150 (N5938_10150) - 2181797..2182222 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  N5938_RS10155 (N5938_10155) - 2182219..2182689 (+) 471 WP_003094664.1 hypothetical protein -
  N5938_RS10160 (N5938_10160) - 2182686..2183375 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  N5938_RS10165 (N5938_10165) - 2183481..2183690 (+) 210 WP_003094660.1 hypothetical protein -
  N5938_RS10170 (N5938_10170) yacG 2183727..2183927 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  N5938_RS10175 (N5938_10175) coaE 2183924..2184535 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  N5938_RS10180 (N5938_10180) pilD 2184532..2185404 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  N5938_RS10185 (N5938_10185) - 2185407..2186625 (-) 1219 Protein_2000 type II secretion system F family protein -
  N5938_RS10190 (N5938_10190) pilB 2186629..2188329 (-) 1701 WP_263901898.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5938_RS10195 (N5938_10195) - 2188560..2189024 (+) 465 WP_023129290.1 pilin -
  N5938_RS10200 (N5938_10200) - 2189339..2189620 (+) 282 WP_263901899.1 hypothetical protein -
  N5938_RS10205 (N5938_10205) - 2189630..2190532 (+) 903 WP_263901900.1 O-antigen ligase family protein -
  N5938_RS10215 (N5938_10215) nadC 2190676..2191524 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62420.36 Da        Isoelectric Point: 5.7102

>NTDB_id=738815 N5938_RS10190 WP_263901898.1 2186629..2188329(-) (pilB) [Pseudomonas aeruginosa strain 2019CK-00034]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFPE
DRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=738815 N5938_RS10190 WP_263901898.1 2186629..2188329(-) (pilB) [Pseudomonas aeruginosa strain 2019CK-00034]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGTGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCACCAGGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCGGTGGTGCGTTT
CGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACCGCG
TACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCACCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGACCTG
GAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGAC
TTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCCGAG
GATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.368

100

0.578
  pilB Acinetobacter baylyi ADP1

56.79

100

0.569
  pilB Legionella pneumophila strain ERS1305867

54.145

100

0.542
  pilF Neisseria gonorrhoeae MS11

50.355

99.647

0.502
  pilB Vibrio cholerae strain A1552

51.083

97.88

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

52.434

94.346

0.495
  pilB Vibrio campbellii strain DS40M4

51.407

94.17

0.484
  pilB/pilB1 Synechocystis sp. PCC 6803

37.439

100

0.408
  pilF Thermus thermophilus HB27

38.313

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

97.173

0.375