Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OEG85_RS05925 Genome accession   NZ_CP107241
Coordinates   1427270..1429006 (+) Length   578 a.a.
NCBI ID   WP_268214355.1    Uniprot ID   A0AA47EUI8
Organism   Xanthomonas hortorum strain Oregano 108     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1422270..1434006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEG85_RS05900 (OEG85_05900) pilC 1422275..1423531 (-) 1257 WP_268214353.1 type II secretion system F family protein Machinery gene
  OEG85_RS05905 (OEG85_05905) - 1423694..1424674 (+) 981 WP_047130215.1 IS5 family transposase -
  OEG85_RS05910 (OEG85_05910) - 1424834..1425978 (+) 1145 WP_268211553.1 IS3 family transposase -
  OEG85_RS05915 (OEG85_05915) pilA 1426281..1426697 (+) 417 WP_268214354.1 pilin Machinery gene
  OEG85_RS05920 (OEG85_05920) - 1426807..1427205 (+) 399 WP_313770514.1 pilin -
  OEG85_RS05925 (OEG85_05925) pilB 1427270..1429006 (+) 1737 WP_268214355.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OEG85_RS05930 (OEG85_05930) pilR 1429348..1430742 (-) 1395 WP_268214356.1 sigma-54 dependent transcriptional regulator Regulator
  OEG85_RS05935 (OEG85_05935) - 1430886..1432499 (-) 1614 WP_006448368.1 HAMP domain-containing sensor histidine kinase -
  OEG85_RS05940 (OEG85_05940) sucC 1432732..1433901 (+) 1170 WP_006450728.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62609.78 Da        Isoelectric Point: 5.7896

>NTDB_id=738722 OEG85_RS05925 WP_268214355.1 1427270..1429006(+) (pilB) [Xanthomonas hortorum strain Oregano 108]
MNVSASANLVGITGIARRLVQDGALEESVARSAMDQAASAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGASLG
NDDDDMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYAADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTAEQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=738722 OEG85_RS05925 WP_268214355.1 1427270..1429006(+) (pilB) [Xanthomonas hortorum strain Oregano 108]
ATGAACGTGAGTGCATCTGCAAATTTAGTTGGTATTACCGGCATTGCGCGTCGTCTAGTCCAAGATGGTGCGCTTGAAGA
GTCTGTTGCTCGGTCTGCGATGGATCAAGCTGCGTCGGCGAAAGTTCCATTGCCACAGTGGTTTGCTGAGAAAAAGCTAG
TTTCAGCCGCTCAGCTGGCTGCAGCTAATGCAGTCGAGTTCGGCATGCCGTTGATGGATGTGTCGGTCTTCGACGCCAGC
CAGAACGCCGTCAAGCTGGTCAGCGAGGAATTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAACTTGGTGGTGGAGCCGA
TTCTGGTCGATGAGGACCAGATCCGGCGCACCCTGGAGCAGTGGCAGGCCAGTAATGCCGCACTGGGCGCGTCGCTCGGC
AACGACGACGACGATATGGGGGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCGTATGAAGACGATTACCGAGTGCGGTTGCGCATCGATGGGTTGTTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTGGGGATCGAAAAGCTGGGCTACGCGGCGGATCAGCAGAAG
CTGTTTTTGGATGCGATCCACAAGCCCTACGGCATGGTGTTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
ATATACGGCGCTGGGCATCCTCAATGATGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTGAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTCGCGGCTGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GATATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCAATCAAGGCAGCGCAGACCGGTCACATGGT
GTTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATTGCGCCGTACAACATCA
CTTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAATAACTGCAAGCGCAAATCGACCTTGCCC
GAGCATGCGTTGCTGGCGGAAGGGTTCACTGCAGAGCAGCTCGCTGCCGGTATCGAGCTGTACGAGGCGGTCGGCTGCGA
CGAGTGCACCGAAGGTTATAAGGGACGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCGGCGCAGAGGATCGGCATACGCGATTTGCGGCAGTCGGCGCTGGTG
AAGGCGGCACATGGGGTAACGAGCTTGGCTGAGATCAATCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.766

99.827

0.547
  pilB Acinetobacter baumannii D1279779

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

52.197

98.443

0.514
  pilF Neisseria gonorrhoeae MS11

48.239

98.27

0.474
  pilB Vibrio cholerae strain A1552

49.094

95.502

0.469
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

46.113

97.924

0.452