Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCT51_RS12260 Genome accession   NZ_CP107009
Coordinates   2612135..2613928 (+) Length   597 a.a.
NCBI ID   WP_263580125.1    Uniprot ID   -
Organism   Halomonas sp. LR3S48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2607135..2618928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCT51_RS12235 (OCT51_12235) - 2607537..2608064 (-) 528 WP_263580122.1 HPP family protein -
  OCT51_RS12240 (OCT51_12240) - 2608192..2608842 (-) 651 WP_318153147.1 anti-virulence regulator CigR family protein -
  OCT51_RS12245 (OCT51_12245) - 2608928..2609347 (+) 420 WP_263580123.1 hypothetical protein -
  OCT51_RS12250 (OCT51_12250) - 2609391..2611181 (-) 1791 WP_263580124.1 polymer-forming cytoskeletal protein -
  OCT51_RS21890 - 2611362..2611739 (-) 378 WP_412031173.1 pilin -
  OCT51_RS12260 (OCT51_12260) pilB 2612135..2613928 (+) 1794 WP_263580125.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCT51_RS12265 (OCT51_12265) pilC 2613995..2615227 (+) 1233 WP_263580126.1 type II secretion system F family protein Machinery gene
  OCT51_RS12270 (OCT51_12270) pilD 2615230..2616057 (+) 828 WP_263580127.1 prepilin peptidase Machinery gene
  OCT51_RS12275 (OCT51_12275) coaE 2616085..2616690 (+) 606 WP_263580128.1 dephospho-CoA kinase -
  OCT51_RS12280 (OCT51_12280) yacG 2616750..2616977 (+) 228 WP_263580129.1 DNA gyrase inhibitor YacG -
  OCT51_RS12285 (OCT51_12285) - 2616980..2617843 (+) 864 WP_412031226.1 DNA replication terminus site-binding protein -
  OCT51_RS12290 (OCT51_12290) - 2617873..2618610 (-) 738 WP_263580130.1 tellurite resistance TerB family protein -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 65300.57 Da        Isoelectric Point: 5.3235

>NTDB_id=737338 OCT51_RS12260 WP_263580125.1 2612135..2613928(+) (pilB) [Halomonas sp. LR3S48]
MNDYRTAPTGAHDSTSGDASQQRSADTEGLHGFARRLVEYGLLSRAAAERAEYEAREAETSLLQHVIDAGLVTARQGTLC
AAWEYGLPLVDLDALRLASLPPANDYPEKLLRKLCVVPLSRRGHRLTVAVPYPSTLALLDELQFATGLSIEAVLCPVDQL
LPVLEAYLAQHESSMMDELAGVDDAVSELEFDEGVDISDERANDAASSGADDAPIVKFVNKILLDAIRRGASDIHFEPYE
TSYRVRMRVDGMLMESARPPFAMRARIAARLKVMARLDISERRLPQDGAIKLKVSKTRSIDFRVNSLPTVYGEKIVLRIL
DPASAQIGIDALGFTPEQRALYEGVLDHPQGMILVTGPTGSGKTVSLYTGLNILNEVQRNICTAEDPVEIKVPGVNQVNV
LPKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVPPFNIASSVSLI
IAQRLARRLCKHCRQEAEIPREALLQEGFTEQEVDSATVYEAVGCKHCTHGYKGRVGIYEVVPISTAMSQLIMRNGNSMD
FDKLAREEGHPDLRRSGLLKVMQGITSLTEVNRVTKD

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=737338 OCT51_RS12260 WP_263580125.1 2612135..2613928(+) (pilB) [Halomonas sp. LR3S48]
ATGAACGACTATCGAACGGCACCGACGGGTGCACACGACTCCACCAGTGGGGATGCCAGCCAGCAGCGCTCGGCCGATAC
CGAGGGCCTGCACGGCTTCGCACGCAGGCTGGTGGAATACGGATTGCTTTCGCGCGCAGCGGCCGAGCGGGCGGAATACG
AGGCACGCGAGGCGGAGACCAGCCTGCTGCAGCACGTGATCGATGCCGGCCTGGTCACGGCCCGCCAAGGCACGCTGTGC
GCCGCCTGGGAGTATGGCCTGCCGCTGGTCGACCTGGACGCGCTGCGCCTGGCGAGCCTGCCCCCGGCCAACGATTATCC
CGAGAAGCTGCTGCGCAAGCTGTGCGTGGTGCCGCTCAGCCGCCGCGGCCACCGGCTGACGGTCGCGGTACCCTACCCTT
CCACCCTGGCCCTGCTCGATGAGCTGCAGTTCGCCACCGGCCTCAGTATCGAAGCCGTGCTGTGCCCGGTGGACCAATTG
CTGCCGGTACTCGAGGCCTACCTGGCCCAGCACGAGTCCAGCATGATGGACGAGCTGGCAGGGGTGGACGATGCCGTCAG
CGAACTGGAGTTCGACGAAGGCGTCGACATCAGCGACGAGCGCGCCAACGATGCTGCCTCGAGCGGTGCCGACGATGCGC
CGATCGTCAAGTTCGTCAACAAGATCCTGCTGGATGCCATTCGCCGCGGCGCTTCGGACATTCACTTCGAGCCCTACGAA
ACCAGCTACCGGGTACGCATGCGCGTGGATGGCATGCTGATGGAATCGGCCCGGCCGCCCTTCGCCATGCGCGCGCGCAT
TGCCGCGCGCCTGAAAGTGATGGCGCGGTTGGACATCTCCGAACGCCGCCTGCCCCAGGATGGCGCGATCAAGCTGAAGG
TGTCGAAGACCCGCTCGATCGATTTCCGCGTGAACTCGCTGCCGACCGTGTACGGCGAGAAGATCGTGCTGCGTATTCTC
GACCCCGCCTCGGCGCAGATCGGTATCGATGCACTGGGCTTCACGCCGGAGCAGCGCGCGCTGTACGAGGGCGTGCTGGA
CCACCCCCAGGGCATGATCCTGGTCACCGGCCCCACCGGCAGCGGCAAAACGGTGTCGCTCTATACCGGGCTGAACATTC
TCAACGAGGTGCAGCGCAATATCTGTACCGCCGAGGATCCGGTGGAGATCAAGGTGCCTGGGGTCAACCAGGTCAACGTG
CTACCCAAGATCGGCCTGGATTTCGCCAGTGCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGGTGATGGTGGGTGA
GATTCGCGACCTTGAAACCGCCGAAATTGCGGTGAAGGCGTCGCAGACCGGCCACCTGGTGCTTTCCACGGTACACACCA
ACTCCGCCGCGGAAACCCTCACCCGCCTGGCCAACATGGGCGTGCCGCCGTTCAACATTGCCAGCTCCGTGAGCCTGATC
ATTGCCCAACGCCTGGCGCGGCGGCTGTGCAAGCATTGCCGCCAGGAGGCGGAGATTCCCCGCGAGGCGCTGCTGCAAGA
GGGCTTCACGGAGCAGGAAGTGGATAGCGCTACCGTGTACGAAGCAGTAGGCTGCAAGCATTGCACCCATGGCTACAAGG
GCCGCGTGGGGATCTATGAAGTGGTACCGATCAGCACGGCCATGAGCCAACTGATCATGCGTAACGGCAACTCCATGGAT
TTCGATAAACTGGCACGGGAAGAGGGCCATCCGGACCTGCGTCGCAGTGGGCTTTTGAAGGTGATGCAGGGGATCACGAG
CCTGACCGAGGTCAACCGGGTCACAAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(221-488)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.545

95.812

0.523
  pilB Acinetobacter baylyi ADP1

53.333

95.477

0.509
  pilB Acinetobacter baumannii D1279779

52.817

95.142

0.503
  pilB Vibrio cholerae strain A1552

51.028

89.615

0.457
  pilB Vibrio campbellii strain DS40M4

48.063

95.142

0.457
  pilF Neisseria gonorrhoeae MS11

47.018

95.477

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

47.943

93.635

0.449
  pilF Thermus thermophilus HB27

37.719

95.477

0.36