Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9799_RS05325 Genome accession   NZ_CP106881
Coordinates   1208645..1210384 (+) Length   579 a.a.
NCBI ID   WP_231043475.1    Uniprot ID   -
Organism   Comamonas endophytica strain 5MLIR     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1203645..1215384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9799_RS05300 (M9799_05300) - 1204535..1205338 (-) 804 WP_231043480.1 ferritin-like domain-containing protein -
  M9799_RS05305 (M9799_05305) - 1205584..1205928 (-) 345 WP_231043479.1 KGG domain-containing protein -
  M9799_RS05310 (M9799_05310) - 1206144..1206653 (-) 510 WP_231043478.1 hypothetical protein -
  M9799_RS05315 (M9799_05315) - 1206761..1208047 (+) 1287 WP_231043477.1 SDR family oxidoreductase -
  M9799_RS05320 (M9799_05320) - 1208059..1208526 (+) 468 WP_231043476.1 DUF2269 domain-containing protein -
  M9799_RS05325 (M9799_05325) pilB 1208645..1210384 (+) 1740 WP_231043475.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M9799_RS05330 (M9799_05330) pilC 1210398..1211606 (+) 1209 WP_231043474.1 type II secretion system F family protein Machinery gene
  M9799_RS05335 (M9799_05335) pilD 1211606..1212487 (+) 882 WP_231043473.1 A24 family peptidase Machinery gene
  M9799_RS05340 (M9799_05340) coaE 1212487..1213119 (+) 633 WP_231043472.1 dephospho-CoA kinase -
  M9799_RS05345 (M9799_05345) zapD 1213180..1213935 (+) 756 WP_231043471.1 cell division protein ZapD -
  M9799_RS05350 (M9799_05350) - 1213943..1214152 (+) 210 WP_231043470.1 DNA gyrase inhibitor YacG -
  M9799_RS05355 (M9799_05355) - 1214149..1214607 (-) 459 WP_231043469.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62793.81 Da        Isoelectric Point: 5.8793

>NTDB_id=736401 M9799_RS05325 WP_231043475.1 1208645..1210384(+) (pilB) [Comamonas endophytica strain 5MLIR]
MPAADSANPPSSSTALPGLGRALISAGTITAQAAEELHRKSQASRTSFISELIGSGTVSPSQLAHTLSAVFGAPLIDLDA
VDPAMLPQGLLAPKLCLTYRVVVLSKRNNRLTIAAADPTDQEAAERIKFTSQMGIDWIIAEYDKLVALCEATHKSAAESL
QSYTASSDFNFDDIKAEEPDEPADNAAASDVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASP
PIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPNRTIDFRVSTLPTLFGEKIVIRILDPESAKMGIEALGYEPEEK
ERLLHAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQD
PDIIMVGEIRDLETADTSIKAAQTGHLVLSTVHTNDAPSTLTRMRNMGIAPFNIASSVILITAQRLARRLCQQCKQPADV
PHQALLDAGYDANDIDGSWVTYKAVGCKACNSGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAAQAKLEGVRSLRESGL
YKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=736401 M9799_RS05325 WP_231043475.1 1208645..1210384(+) (pilB) [Comamonas endophytica strain 5MLIR]
ATGCCTGCTGCCGATTCCGCCAACCCGCCTTCCTCCAGCACGGCGCTTCCCGGCCTGGGTCGCGCCCTGATTTCTGCCGG
CACGATCACGGCCCAGGCAGCCGAGGAACTGCACCGGAAATCCCAGGCTTCGCGCACCAGCTTCATCTCCGAGCTCATCG
GCTCCGGGACGGTCTCTCCCTCCCAGCTGGCGCATACCCTGTCCGCCGTGTTCGGCGCGCCGCTGATAGACCTGGACGCG
GTCGATCCCGCGATGCTGCCACAGGGGCTGCTGGCACCCAAGCTCTGCCTGACCTACCGCGTGGTGGTGCTGAGCAAGCG
CAACAACCGGCTGACGATCGCCGCTGCCGACCCCACCGACCAGGAAGCGGCGGAACGCATCAAGTTCACGTCGCAGATGG
GGATCGACTGGATCATTGCCGAGTACGACAAGCTGGTGGCGCTGTGCGAGGCCACGCACAAGAGCGCCGCCGAGTCGCTG
CAGTCGTACACCGCCTCCAGCGACTTCAACTTCGACGACATCAAGGCCGAGGAGCCCGACGAACCCGCCGACAATGCCGC
GGCATCGGACGTCGAAGACGCGCCGGTCGTGAAATTCCTGCACAAGATGCTGCTCGATGCGTTCAACATGCGCGCCTCCG
ACCTGCATTTCGAGCCCTACGAGCACCAGTACCGGGTGCGCTTCCGCATCGACGGCGAGCTGCGCGAGATCGCCTCGCCG
CCCATTGCCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCGCGCCTGGACATCTCCGAGAAGCGCGTGCCGCA
GGATGGGCGCATGAAGCTCAAGGTCGGTCCCAACCGCACCATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCG
AGAAGATCGTGATCCGTATCCTGGACCCCGAGAGCGCCAAGATGGGCATCGAGGCGCTGGGCTACGAGCCCGAGGAGAAG
GAGCGGCTGCTGCACGCCATCAGCCGCCCCTACGGCATGATCCTGGTCACGGGCCCCACGGGCTCGGGCAAGACGGTGTC
GCTCTACACCTGCCTGAACCTGCTGAACAAGCCGGGCGTCAACATCGCGACCGCCGAGGACCCCTCGGAAATCAACCTGC
CCGGCGTCAACCAGGTCAACGTCAACGAAAAGGCCGGCCTGACCTTCGCCACCGCGCTCAAGGCCTTCCTGCGCCAGGAT
CCCGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGATACCTCGATCAAGGCCGCCCAGACCGGCCACCT
GGTGCTGTCCACCGTGCACACCAACGACGCGCCCAGCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCCTTCAACA
TCGCCTCGAGCGTCATCCTGATCACCGCCCAGCGCCTGGCGCGGCGCCTGTGCCAGCAATGCAAGCAGCCGGCCGACGTG
CCGCACCAGGCGCTGCTGGACGCCGGCTACGACGCCAACGACATCGACGGCAGCTGGGTGACCTACAAGGCCGTGGGCTG
CAAGGCCTGCAACAGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTCATGCCGGTCTCCGAGGAAATCCAGCGCATCA
TCCTGCGCGACGGCAGCGCGCTCGAGATCGCGGCGCAGGCCAAGCTCGAGGGGGTGCGCTCGCTGCGCGAATCGGGCCTG
TACAAGGCGCGGCTGGGACTGACTTCACTGGAAGAGGTGCTGGCGGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

52.595

99.827

0.525
  pilB Acinetobacter baumannii D1279779

52.88

98.964

0.523
  pilF Neisseria gonorrhoeae MS11

51.504

97.582

0.503
  pilB Legionella pneumophila strain ERS1305867

49.381

97.582

0.482
  pilB Vibrio cholerae strain A1552

45.965

98.446

0.453
  pilB Vibrio parahaemolyticus RIMD 2210633

44.84

97.064

0.435
  pilB Vibrio campbellii strain DS40M4

44.523

97.755

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.292

97.582

0.383
  pilF Thermus thermophilus HB27

38.938

97.582

0.38