Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N8I74_RS06115 Genome accession   NZ_CP106753
Coordinates   1357220..1358929 (-) Length   569 a.a.
NCBI ID   WP_263125970.1    Uniprot ID   -
Organism   Chitiniphilus purpureus strain CD1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1352220..1363929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8I74_RS06085 (N8I74_06085) - 1352544..1353293 (-) 750 WP_263125963.1 GNAT family N-acetyltransferase -
  N8I74_RS06090 (N8I74_06090) - 1353477..1353662 (-) 186 WP_263125964.1 DNA gyrase inhibitor YacG -
  N8I74_RS06095 (N8I74_06095) zapD 1353659..1354531 (-) 873 WP_263125966.1 cell division protein ZapD -
  N8I74_RS06100 (N8I74_06100) coaE 1354489..1355100 (-) 612 WP_263125967.1 dephospho-CoA kinase -
  N8I74_RS06105 (N8I74_06105) pilD 1355106..1355972 (-) 867 WP_263125968.1 A24 family peptidase Machinery gene
  N8I74_RS06110 (N8I74_06110) pilC 1355972..1357204 (-) 1233 WP_408611867.1 type II secretion system F family protein Machinery gene
  N8I74_RS06115 (N8I74_06115) pilB 1357220..1358929 (-) 1710 WP_263125970.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N8I74_RS06120 (N8I74_06120) - 1359158..1359967 (-) 810 WP_263125971.1 inner membrane protein YpjD -
  N8I74_RS06125 (N8I74_06125) ffh 1360049..1361398 (+) 1350 WP_263125972.1 signal recognition particle protein -
  N8I74_RS06130 (N8I74_06130) rpsP 1361503..1361751 (+) 249 WP_263125973.1 30S ribosomal protein S16 -
  N8I74_RS06135 (N8I74_06135) rimM 1361819..1362301 (+) 483 WP_263126723.1 ribosome maturation factor RimM -
  N8I74_RS06140 (N8I74_06140) trmD 1362310..1363125 (+) 816 WP_408611868.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  N8I74_RS06145 (N8I74_06145) rplS 1363130..1363510 (+) 381 WP_263125974.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61926.02 Da        Isoelectric Point: 5.2043

>NTDB_id=734468 N8I74_RS06115 WP_263125970.1 1357220..1358929(-) (pilB) [Chitiniphilus purpureus strain CD1]
MSTATAPISGLARLLVQHGRLLEAEAEALHNNAVNTKHSFIEQLISSRKMSAREVAEFGAQTFGYPLLDIDQIDTSYIPS
GVLDQKVMQSNRLVPLFKRGSKLFVGISDITNLQALEEVRFQTGLQVEPIVAEDNKLAVLIDKLVEASGANLKNIAVDEA
DLDIQGGDEEQAGDTANTDVDDAPVVKYLQKILLDAINAGASDIHFEPYEKFYRIRYRQDGVLREIAQPPLAIKEKLASR
IKVISKLDISEKRVPQDGRMKLVLSKSRAIDFRVSTLPTLHGEKICIRILDPTSATLGVDALGYEPDQKAALLDAIHRPY
GMVLVTGPTGSGKTVSLYTCLNILNEPGINISTAEDPAEINLAGINQVNVNDKAGLTFSIALKAFLRQDPDVIMVGEIRD
LETADIAIKAAQTGHMVFSTLHTNDAPTTLTRLVNMGVAPFNVASSVILISAQRLARRLCSSCKAPVDIPPEALLDAGYR
EAELDGSWVPYRAVGCDACKGTGYKGRVGIYQVMPISDEMNRIIMKNGTAIDIADQARREGVRDLRQSGLLKVKQGMTSL
EEVMAVTNE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=734468 N8I74_RS06115 WP_263125970.1 1357220..1358929(-) (pilB) [Chitiniphilus purpureus strain CD1]
ATGTCGACTGCCACTGCGCCTATTTCCGGTCTCGCACGTCTGCTGGTGCAACATGGCCGCCTTCTGGAAGCGGAAGCGGA
AGCGCTGCACAACAACGCTGTCAACACCAAGCACAGCTTCATCGAACAGCTGATCAGCAGCCGCAAGATGTCCGCCCGGG
AGGTGGCCGAATTCGGCGCGCAGACATTCGGCTACCCTTTACTCGATATCGATCAGATCGATACCAGCTACATCCCTTCG
GGCGTGCTCGACCAGAAGGTGATGCAATCCAACCGGCTGGTGCCGCTGTTCAAGCGCGGCTCCAAATTGTTCGTCGGCAT
CTCCGACATCACGAATCTGCAGGCGCTCGAGGAGGTCCGCTTTCAGACCGGCCTGCAGGTCGAGCCCATCGTCGCCGAAG
ACAACAAGCTGGCCGTCCTGATCGACAAACTGGTCGAAGCCAGCGGCGCCAACCTCAAGAACATTGCCGTCGACGAAGCC
GATCTCGATATCCAGGGCGGTGACGAGGAGCAGGCAGGCGATACCGCCAACACGGACGTCGACGACGCGCCGGTGGTCAA
GTATCTGCAAAAGATTCTGCTCGATGCGATCAATGCCGGCGCATCGGATATCCATTTCGAACCTTACGAGAAATTCTATC
GGATCCGTTATCGCCAGGACGGCGTACTGCGTGAAATCGCCCAGCCCCCTCTGGCAATCAAGGAAAAGCTCGCTTCCCGG
ATCAAGGTGATCTCGAAACTGGATATCTCGGAAAAGCGCGTACCGCAGGACGGCCGGATGAAGCTGGTGCTCTCCAAGAG
CCGGGCAATCGATTTCCGGGTCTCCACGCTGCCCACGCTGCATGGCGAAAAGATCTGTATCCGGATTCTCGACCCCACCT
CGGCGACCTTGGGCGTGGATGCGCTTGGCTATGAACCGGACCAGAAGGCGGCGCTGCTCGACGCGATCCACCGCCCCTAC
GGTATGGTGCTCGTCACCGGTCCGACGGGCTCGGGCAAGACCGTGTCGCTCTATACCTGCCTCAATATCCTCAATGAACC
GGGAATCAACATTTCCACGGCCGAAGATCCGGCGGAAATCAACTTGGCGGGCATCAATCAGGTCAACGTCAACGATAAGG
CCGGCCTCACCTTCTCGATCGCACTCAAGGCATTCCTGCGACAGGATCCGGACGTGATCATGGTCGGCGAAATCCGCGAC
CTGGAAACCGCCGACATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGTTCTCGACCCTGCACACCAACGATGCGCC
GACCACGCTGACCCGGCTGGTGAACATGGGGGTGGCGCCCTTCAACGTCGCCTCGTCGGTGATCCTGATCAGCGCGCAGC
GTCTTGCACGCCGGTTGTGCTCCAGTTGCAAGGCGCCGGTGGATATTCCGCCTGAGGCATTGCTGGATGCCGGGTATAGG
GAGGCGGAGCTGGACGGCAGCTGGGTGCCCTACCGTGCGGTGGGATGCGACGCATGCAAGGGAACGGGTTACAAGGGCCG
GGTCGGGATCTATCAGGTGATGCCGATCTCGGATGAGATGAACCGCATCATCATGAAGAACGGCACGGCCATCGACATCG
CCGACCAGGCCCGCCGCGAAGGCGTGCGCGACCTGCGGCAATCGGGCCTGCTCAAGGTCAAGCAGGGGATGACTTCGCTT
GAGGAAGTCATGGCAGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.557
  pilF Neisseria gonorrhoeae MS11

55.872

98.77

0.552
  pilB Acinetobacter baylyi ADP1

54.355

100

0.548
  pilB Legionella pneumophila strain ERS1305867

53.147

100

0.534
  pilB Vibrio parahaemolyticus RIMD 2210633

47.434

99.297

0.471
  pilB Vibrio cholerae strain A1552

50.376

93.497

0.471
  pilB Vibrio campbellii strain DS40M4

47.08

99.297

0.467
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.19

92.267

0.371
  pilF Thermus thermophilus HB27

39.548

93.322

0.369