Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N7220_RS06030 Genome accession   NZ_CP106736
Coordinates   1326795..1328504 (+) Length   569 a.a.
NCBI ID   WP_283150556.1    Uniprot ID   -
Organism   Silvimonas soli strain 17-6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1321795..1333504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7220_RS06005 rimM 1322002..1322553 (-) 552 WP_283150551.1 ribosome maturation factor RimM -
  N7220_RS06010 rpsP 1322564..1322815 (-) 252 WP_283150552.1 30S ribosomal protein S16 -
  N7220_RS06015 ffh 1322922..1324265 (-) 1344 WP_283150553.1 signal recognition particle protein -
  N7220_RS06020 - 1324347..1325153 (+) 807 WP_283150554.1 inner membrane protein YpjD -
  N7220_RS06025 - 1325226..1326494 (+) 1269 WP_283150555.1 HlyC/CorC family transporter -
  N7220_RS06030 pilB 1326795..1328504 (+) 1710 WP_283150556.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N7220_RS06035 pilC 1328569..1329807 (+) 1239 WP_283150557.1 type II secretion system F family protein Machinery gene
  N7220_RS06040 pilD 1329924..1330682 (+) 759 WP_390901568.1 prepilin peptidase Machinery gene
  N7220_RS06045 coaE 1330708..1331316 (+) 609 WP_283150559.1 dephospho-CoA kinase -
  N7220_RS06050 zapD 1331480..1332238 (+) 759 WP_283150560.1 cell division protein ZapD -
  N7220_RS06055 - 1332281..1332478 (+) 198 WP_283150561.1 DNA gyrase inhibitor YacG -
  N7220_RS06060 - 1332571..1333323 (+) 753 WP_283150562.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62532.04 Da        Isoelectric Point: 5.3207

>NTDB_id=734198 N7220_RS06030 WP_283150556.1 1326795..1328504(+) (pilB) [Silvimonas soli strain 17-6]
MSTTSAPISGLARLLVQHGRLAESDIEAIHQTPGEGKNSFIEQLIQSKKMTGKEIAEFSSQAFGYPLLDLDQIDDSYIPQ
GVLDPKLMQAQRLVPLFKRGTRLFIGISDITNMQALEEVRFQTGQQIEPIVVEDAKLLALLFKLIEASGASIKDLQVNDA
ELELSGGEEENKPEVASVDVDDAPVVKYIQKILLDAIKAEASDIHFEPYEKFYRIRYRQDGILREVAQPPLAIKEKIASR
IKVISKLDISEKRVPQDGRMKLVLSRTRAIDFRVSTLPTLHGEKICIRILDPSSATLGIDALGYDPDQKEYLMRAINRPY
GMVLVTGPTGSGKTVSLYTCLNILNVPGTNISTAEDPAEINLPGVNQVNVNEKAGLTFSVALKAFLRQDPDVIMVGEIRD
LETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLLNMGVAPFNVASSVILITAQRLGRRLCSQCKVPMDIPHEALLDAGFT
EADLDGSWTPYRPVGCDHCKGTGYKGRVGIYQVMPISDEMNRIIMKNGTAIDIADQARREGVRDLRLSGLIKVKLGVTSL
EEVMAITNE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=734198 N7220_RS06030 WP_283150556.1 1326795..1328504(+) (pilB) [Silvimonas soli strain 17-6]
ATGTCCACAACTTCAGCCCCTATTTCCGGCTTGGCACGCCTGCTGGTTCAGCACGGGCGCTTGGCCGAATCCGATATCGA
AGCCATTCACCAGACGCCAGGTGAAGGCAAAAACTCGTTCATCGAGCAGCTCATCCAGAGCAAAAAGATGACGGGCAAAG
AAATTGCCGAGTTCAGCTCGCAGGCCTTTGGTTATCCATTGCTGGATCTGGACCAGATTGACGACAGTTATATTCCACAA
GGGGTGCTTGATCCGAAACTGATGCAAGCGCAGCGGTTGGTGCCGCTATTCAAGCGCGGCACGCGGCTGTTTATCGGCAT
TTCTGACATCACCAACATGCAGGCGCTGGAAGAAGTGCGCTTTCAGACCGGCCAGCAAATCGAACCCATCGTGGTCGAAG
ATGCCAAGTTGCTGGCCTTGCTGTTCAAGCTGATTGAGGCCTCGGGCGCTTCCATCAAAGACCTGCAAGTCAATGACGCC
GAACTTGAATTAAGCGGCGGTGAAGAAGAGAACAAGCCAGAAGTCGCATCGGTCGATGTTGACGATGCGCCGGTGGTGAA
ATATATCCAGAAAATCCTGCTGGATGCGATCAAGGCCGAGGCTTCGGATATTCACTTCGAGCCTTACGAAAAGTTCTACC
GCATCCGCTATCGCCAGGACGGCATTTTGCGTGAGGTAGCGCAGCCGCCACTGGCCATCAAAGAAAAAATCGCTTCGCGG
ATCAAGGTGATCTCCAAGCTGGATATCTCGGAAAAACGCGTGCCGCAAGATGGCCGGATGAAACTGGTGCTATCGCGCAC
CCGTGCCATTGATTTCCGGGTCTCCACGCTGCCGACTTTGCACGGCGAAAAGATTTGTATCCGGATTCTGGACCCGTCAT
CAGCCACGCTCGGCATCGACGCGCTCGGCTACGACCCGGATCAGAAAGAATATCTGATGCGTGCAATCAACCGGCCTTAC
GGCATGGTGCTGGTGACCGGCCCAACGGGCTCCGGCAAAACGGTGTCGTTGTACACCTGTTTGAATATTCTGAATGTGCC
GGGCACCAACATTTCGACGGCGGAAGACCCGGCCGAAATCAACTTGCCCGGCGTGAATCAGGTGAACGTCAATGAAAAAG
CCGGGCTGACGTTCTCCGTCGCGCTCAAGGCCTTCTTGCGGCAAGATCCGGACGTGATCATGGTCGGCGAAATCCGTGAC
CTGGAAACCGCCGACATTGCGATCAAGGCGGCGCAGACGGGTCACATGGTGTTCTCTACCCTGCACACCAACGACGCGCC
GAGCACGCTCACCCGTTTGTTGAATATGGGTGTCGCACCATTTAACGTCGCCTCGTCGGTCATTTTGATCACGGCGCAGC
GTTTGGGCCGGCGTTTGTGTTCGCAATGCAAGGTACCAATGGACATCCCGCACGAGGCATTGCTGGATGCCGGTTTTACT
GAAGCCGACCTGGATGGCAGCTGGACACCTTATCGCCCGGTGGGCTGTGATCACTGTAAAGGTACCGGCTATAAGGGCCG
GGTGGGCATTTATCAGGTCATGCCGATTTCTGACGAAATGAACCGTATCATCATGAAAAACGGCACCGCCATCGATATTG
CCGATCAGGCCAGACGCGAAGGTGTGCGCGATTTGCGTTTGTCCGGGCTGATCAAGGTCAAGCTGGGAGTGACTTCGCTA
GAAGAAGTCATGGCAATTACCAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.211

100

0.543
  pilB Acinetobacter baylyi ADP1

54.13

100

0.541
  pilF Neisseria gonorrhoeae MS11

54.448

98.77

0.538
  pilB Legionella pneumophila strain ERS1305867

51.748

100

0.52
  pilB Vibrio parahaemolyticus RIMD 2210633

46.946

100

0.473
  pilB Vibrio cholerae strain A1552

47.153

98.77

0.466
  pilB Vibrio campbellii strain DS40M4

45.471

98.946

0.45
  pilF Thermus thermophilus HB27

38.028

99.824

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.571

98.418

0.38