Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N8E87_RS02485 Genome accession   NZ_CP104917
Coordinates   500442..501866 (+) Length   474 a.a.
NCBI ID   WP_264245772.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain AP-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495442..506866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8E87_RS02460 (N8E87_02460) lgt 496018..496818 (-) 801 WP_264245746.1 prolipoprotein diacylglyceryl transferase -
  N8E87_RS02465 (N8E87_02465) - 496828..497619 (-) 792 WP_264245747.1 sulfite exporter TauE/SafE family protein -
  N8E87_RS02470 (N8E87_02470) rppH 497622..498215 (-) 594 WP_264245767.1 RNA pyrophosphohydrolase -
  N8E87_RS02475 (N8E87_02475) ampD 499304..499858 (-) 555 WP_264245770.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N8E87_RS02480 (N8E87_02480) pilA 499998..500429 (+) 432 WP_035687024.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  N8E87_RS02485 (N8E87_02485) pilB 500442..501866 (+) 1425 WP_264245772.1 GspE/PulE family protein Machinery gene
  N8E87_RS02490 (N8E87_02490) pyrH 502116..502832 (+) 717 WP_035687020.1 UMP kinase -
  N8E87_RS02495 (N8E87_02495) frr 502857..503414 (+) 558 WP_264245774.1 ribosome recycling factor -
  N8E87_RS02500 (N8E87_02500) ispC 503596..504792 (+) 1197 WP_264245775.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  N8E87_RS02505 (N8E87_02505) uppS 504946..505674 (+) 729 WP_046097581.1 polyprenyl diphosphate synthase -
  N8E87_RS02510 (N8E87_02510) - 505678..506541 (+) 864 WP_035687012.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 53776.54 Da        Isoelectric Point: 5.2173

>NTDB_id=733498 N8E87_RS02485 WP_264245772.1 500442..501866(+) (pilB) [Avibacterium paragallinarum strain AP-2]
MAEYSLDEDDIVYAVDGKCFEITPHLWQQNKQQKDILIRYLAVPLQEDEHTLWLGIDDLTNLSACESFSFLTGKIVEPIL
LSEQDLKSLLQKLSPTLVEEPALLDFYQSSISPYSENQPEKLDEPIIQILNKVFESAMQLNASDIHLEPQNEQLQIRFRI
DGVLQHQPPISKQMSQRLISRLKLLARLELSETRLPQDGRFHFKTTFSDILDFRISTLPTYYGEKAVLRLQKNKPVTLDF
SELGMTAIQRKTLQDALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIGLDFN
RLLRTFLRQDPDIIMLGEIRDAESAAIALRAAQTGHLVLSTLHTNNAHSAIARLLQLGIQQYEIESCLLLVIAQRLVRKK
CLHKTKNCECYQGYSGRTGIYQFLQAIFDSNTGQISYQTDYPSLWDSASHKIAQGITDIAELERVLGRHTKAEL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=733498 N8E87_RS02485 WP_264245772.1 500442..501866(+) (pilB) [Avibacterium paragallinarum strain AP-2]
ATGGCTGAGTATTCTTTAGACGAGGACGACATTGTATATGCTGTTGATGGAAAATGCTTTGAAATAACACCGCACTTATG
GCAACAAAATAAGCAGCAAAAAGATATTTTAATACGTTATTTAGCAGTACCTTTACAGGAGGATGAACATACACTTTGGC
TTGGTATTGATGACTTAACAAATCTATCGGCTTGTGAAAGTTTTTCATTTCTAACAGGAAAAATAGTTGAGCCGATTTTG
TTATCAGAACAAGATTTAAAATCGCTATTACAGAAGTTATCTCCCACATTAGTAGAAGAGCCGGCATTACTTGATTTTTA
TCAATCGTCTATTTCACCTTATAGCGAAAATCAACCAGAAAAACTTGATGAACCAATTATTCAAATACTAAATAAAGTTT
TTGAATCGGCAATGCAATTAAATGCCTCAGATATTCATTTAGAGCCACAGAATGAGCAATTGCAGATCCGTTTTCGTATT
GACGGGGTATTGCAACATCAGCCTCCTATTTCTAAACAAATGAGCCAACGTTTGATTTCTCGTTTAAAACTTCTAGCTCG
TCTTGAGCTTAGCGAAACTCGCTTACCACAAGATGGAAGATTTCATTTCAAAACGACATTTTCCGATATATTAGACTTCC
GTATATCGACATTACCTACATATTATGGAGAAAAAGCGGTACTACGTTTACAAAAAAATAAACCTGTTACCTTGGATTTT
TCTGAGCTGGGGATGACAGCGATACAACGAAAAACATTACAAGATGCTTTATCTCAACCTCAAGGACTCATTTTAGTAAC
GGGGCCAACCGGGAGTGGAAAAAGTATTACGCTCTATACAGCATTAGAATGGCTTAATCAATCGGAAAAACATATTCTAA
CAGCAGAAGATCCGATTGAAATAGAACTTCCAGGGATTATCCAAACGCAAATTAACCCACAAATTGGTTTAGATTTTAAC
CGTTTGTTGCGTACATTTTTACGTCAGGATCCAGACATTATTATGCTTGGTGAGATCCGTGATGCAGAAAGTGCAGCGAT
TGCGTTACGTGCAGCACAAACTGGACATTTAGTCCTTTCAACATTACATACAAATAATGCACATTCAGCTATTGCTCGCT
TGTTACAGCTTGGTATTCAACAATATGAAATCGAAAGTTGTTTATTATTAGTGATTGCCCAGCGTTTAGTCCGTAAAAAA
TGTCTACACAAAACGAAAAATTGCGAATGTTATCAAGGTTATAGTGGGCGTACTGGTATTTATCAATTTTTACAAGCTAT
TTTTGATTCAAATACAGGGCAAATTTCTTATCAAACCGATTATCCCTCATTATGGGATAGTGCTTCACATAAAATAGCAC
AAGGTATCACAGATATTGCTGAATTAGAACGAGTTTTAGGTAGGCATACAAAAGCTGAACTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(132-399)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

64.482

99.789

0.643
  pilB Haemophilus influenzae 86-028NP

63.713

100

0.637
  pilB Glaesserella parasuis strain SC1401

57.053

100

0.572
  pilB Legionella pneumophila strain ERS1305867

39.431

100

0.409
  pilB Vibrio campbellii strain DS40M4

40

100

0.409
  pilB Vibrio parahaemolyticus RIMD 2210633

39.588

100

0.405
  pilB Vibrio cholerae strain A1552

39.212

100

0.399
  pilB Acinetobacter baylyi ADP1

37.425

100

0.392
  pilF Neisseria gonorrhoeae MS11

37.107

100

0.373
  pilB Acinetobacter baumannii D1279779

36.289

100

0.371