Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N8E87_RS02480 Genome accession   NZ_CP104917
Coordinates   499998..500429 (+) Length   143 a.a.
NCBI ID   WP_035687024.1    Uniprot ID   A0A0F5ESJ8
Organism   Avibacterium paragallinarum strain AP-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494998..505429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8E87_RS02455 (N8E87_02455) - 495155..496006 (-) 852 WP_264245744.1 thymidylate synthase -
  N8E87_RS02460 (N8E87_02460) lgt 496018..496818 (-) 801 WP_264245746.1 prolipoprotein diacylglyceryl transferase -
  N8E87_RS02465 (N8E87_02465) - 496828..497619 (-) 792 WP_264245747.1 sulfite exporter TauE/SafE family protein -
  N8E87_RS02470 (N8E87_02470) rppH 497622..498215 (-) 594 WP_264245767.1 RNA pyrophosphohydrolase -
  N8E87_RS02475 (N8E87_02475) ampD 499304..499858 (-) 555 WP_264245770.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N8E87_RS02480 (N8E87_02480) pilA 499998..500429 (+) 432 WP_035687024.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  N8E87_RS02485 (N8E87_02485) pilB 500442..501866 (+) 1425 WP_264245772.1 GspE/PulE family protein Machinery gene
  N8E87_RS02490 (N8E87_02490) pyrH 502116..502832 (+) 717 WP_035687020.1 UMP kinase -
  N8E87_RS02495 (N8E87_02495) frr 502857..503414 (+) 558 WP_264245774.1 ribosome recycling factor -
  N8E87_RS02500 (N8E87_02500) ispC 503596..504792 (+) 1197 WP_264245775.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15205.70 Da        Isoelectric Point: 9.8115

>NTDB_id=733497 N8E87_RS02480 WP_035687024.1 499998..500429(+) (pilA) [Avibacterium paragallinarum strain AP-2]
MQNPTHLKIKKGFTLIELMIVIAIVAILATIAIPAYQNYTKKAAISELIQAASPYRSEVELCIYNTGATTKCNAGSNGIQ
KKLTNKGKVASIDVQKGTITVKGKDNLSDITYSLKASGDASKGVSWTVNCGKSVEFFPAGFCG

Nucleotide


Download         Length: 432 bp        

>NTDB_id=733497 N8E87_RS02480 WP_035687024.1 499998..500429(+) (pilA) [Avibacterium paragallinarum strain AP-2]
ATGCAAAACCCAACCCATTTAAAAATCAAAAAAGGCTTTACTTTGATTGAACTGATGATCGTGATTGCGATTGTGGCGAT
TTTAGCGACCATTGCGATTCCTGCCTATCAAAATTACACTAAGAAAGCGGCAATATCTGAGCTAATTCAAGCCGCAAGCC
CCTATCGTTCAGAAGTGGAATTATGTATTTATAATACGGGGGCTACCACGAAATGTAATGCGGGTTCAAATGGAATTCAG
AAAAAATTAACGAATAAAGGGAAAGTGGCTTCTATTGATGTACAAAAAGGCACGATTACGGTTAAAGGAAAAGATAATCT
TTCTGATATTACTTATTCATTAAAAGCCAGTGGAGATGCCTCAAAAGGTGTATCTTGGACGGTAAATTGTGGAAAATCTG
TAGAGTTTTTCCCAGCGGGATTTTGCGGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5ESJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

59.31

100

0.601
  pilA Haemophilus influenzae 86-028NP

57.931

100

0.587
  pilA Glaesserella parasuis strain SC1401

46.809

98.601

0.462
  pilA Vibrio campbellii strain DS40M4

34.615

100

0.378
  pilA Ralstonia pseudosolanacearum GMI1000

37.324

99.301

0.371