Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5P16_RS00325 Genome accession   NZ_CP104734
Coordinates   76021..77733 (+) Length   570 a.a.
NCBI ID   WP_004920473.1    Uniprot ID   -
Organism   Acinetobacter baylyi strain JAT2044     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 71021..82733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5P16_RS00290 (N5P16_00290) nusA 71496..72980 (-) 1485 WP_004920463.1 transcription termination factor NusA -
  N5P16_RS00295 (N5P16_00295) rimP 73016..73540 (-) 525 WP_004920465.1 ribosome maturation factor RimP -
  N5P16_RS00315 (N5P16_00315) secG 74620..74949 (-) 330 WP_004920467.1 preprotein translocase subunit SecG -
  N5P16_RS00320 (N5P16_00320) tpiA 74959..75756 (-) 798 WP_004920470.1 triose-phosphate isomerase -
  N5P16_RS00325 (N5P16_00325) pilB 76021..77733 (+) 1713 WP_004920473.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5P16_RS00330 (N5P16_00330) pilC 77762..78988 (+) 1227 WP_004920476.1 type II secretion system F family protein Machinery gene
  N5P16_RS00335 (N5P16_00335) pilD 78988..79848 (+) 861 WP_004920478.1 prepilin peptidase Machinery gene
  N5P16_RS00340 (N5P16_00340) coaE 79854..80441 (+) 588 WP_004920482.1 dephospho-CoA kinase -
  N5P16_RS00345 (N5P16_00345) - 80438..81355 (-) 918 WP_004920488.1 DMT family transporter -
  N5P16_RS00350 (N5P16_00350) rlmB 81396..82142 (-) 747 WP_004920490.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  N5P16_RS00355 (N5P16_00355) - 82242..82568 (-) 327 WP_004920494.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63976.80 Da        Isoelectric Point: 5.3446

>NTDB_id=731673 N5P16_RS00325 WP_004920473.1 76021..77733(+) (pilB) [Acinetobacter baylyi strain JAT2044]
MSAFTTPPKFSGFIRRLVEEGYVNAQNMQQALEKAKKFKQDIVPYLIDNFSISPLTIAEIISLEFGEPLLDLGVFDPALF
LKDKIDEKLIQKYRIMPLVHRGHVLYVATSNPTNIEAMDAIRFNSKLKVEPIIVEHDKLERLLSEHFVEETHFNFDTEEL
DLDVEVDPHTTDDDDEDDKLKDEAPIVKYINKLLIDAIRMSASDLHFEPYEKSYRVRYRVDGVLRLIATPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKMSKSKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKALFMEALNKP
QGMLLITGPTGSGKTVSLYTGLNILNTEHANISTAEDPVEINLEGVNQVNVNPKVGLTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRNMGVASFNIATSVNLVIAQRLARRLCSQCKRPIQVPERSLLEMGF
TPEDLAQPEFQIFEPVGCHDCREGYKGRVGIYEVMKITPEISKIIMEDGNALEIAATAETLGFNNLRRSGLKKVMQGVTS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=731673 N5P16_RS00325 WP_004920473.1 76021..77733(+) (pilB) [Acinetobacter baylyi strain JAT2044]
ATGTCAGCATTTACAACACCACCTAAGTTTTCGGGGTTTATTCGGCGTTTAGTCGAAGAAGGCTATGTGAATGCACAAAA
TATGCAGCAGGCTTTGGAAAAAGCAAAAAAATTTAAACAAGATATTGTGCCCTACTTGATTGATAATTTTTCAATCTCTC
CACTCACCATTGCAGAAATCATTTCTTTAGAGTTTGGAGAACCATTACTTGATCTGGGTGTTTTTGATCCCGCTTTATTT
TTAAAAGATAAAATTGATGAAAAACTGATCCAGAAATATCGAATCATGCCATTGGTGCATCGCGGGCATGTGTTGTATGT
CGCAACCAGTAATCCAACCAACATTGAGGCAATGGATGCGATTCGATTTAATAGCAAGCTCAAAGTTGAACCAATTATTG
TTGAACATGACAAATTAGAGCGATTGCTTTCAGAACATTTTGTTGAGGAAACTCATTTTAATTTTGATACAGAAGAGCTT
GATCTGGATGTTGAAGTCGATCCACATACAACGGATGACGACGATGAGGATGACAAACTTAAAGACGAAGCACCTATTGT
TAAATACATTAATAAGTTACTGATTGATGCGATACGCATGAGTGCTTCGGATTTACACTTTGAGCCCTATGAGAAAAGCT
ATCGGGTCCGTTATCGGGTAGATGGTGTACTGCGGTTGATTGCAACACCTCCCCTGCAACTGGCAACACGACTGGCCTCA
CGTTTGAAAGTCATGTCACAAATGGATATTTCGGAAAAACGTGTTCCGCAAGATGGCCGTATTAAACTCAAAATGTCTAA
AAGTAAAACCATTGATTTTCGGGTCAATTCTTTGCCGACATTATTTGGGGAAAAAATTGTTCTTCGGATTCTTGATCCAG
CCAGTGCCATGCTCGGTATTGATGCACTCGGTTATGAACCCGAGCAAAAAGCACTATTTATGGAGGCTTTAAACAAACCG
CAAGGGATGCTTCTAATTACGGGACCAACGGGTTCAGGTAAAACCGTATCTTTATATACAGGCTTAAATATACTCAATAC
CGAACATGCCAATATTTCCACAGCAGAAGATCCAGTAGAAATCAATCTCGAAGGCGTCAATCAGGTCAATGTCAATCCAA
AGGTTGGACTGACATTTGCTGCTGCATTAAGATCCTTTTTACGTCAGGATCCTGATATTATTATGGTCGGGGAAATACGT
GATCTGGAAACCGCTGAGATCGCCATTAAAGCAGCTCAAACAGGTCATTTGGTCATGTCGACGTTGCATACCAATAATGC
AGCAGAAACACTGACTCGACTCAGAAATATGGGCGTTGCTTCTTTTAATATTGCAACCTCAGTGAATCTGGTGATCGCAC
AACGTCTTGCTAGGCGTTTATGCAGCCAGTGCAAACGACCGATTCAGGTTCCAGAGCGAAGTTTGCTGGAAATGGGCTTT
ACGCCAGAAGATTTAGCTCAACCAGAATTTCAGATTTTTGAGCCTGTAGGGTGTCATGACTGTCGCGAAGGCTATAAAGG
CCGTGTGGGGATTTACGAAGTCATGAAAATTACACCAGAAATATCAAAAATTATTATGGAAGATGGTAATGCGCTAGAAA
TTGCCGCAACAGCCGAAACATTGGGGTTCAATAATTTACGTCGGTCGGGTCTGAAAAAAGTGATGCAGGGGGTCACCTCG
TTACAGGAAATTAATCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

100

100

1
  pilB Acinetobacter baumannii D1279779

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

49.742

100

0.507
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

48.115

97.719

0.47
  pilB Vibrio cholerae strain A1552

50.772

90.877

0.461
  pilB Vibrio campbellii strain DS40M4

49.903

90.702

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

34.084

100

0.372