Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5P21_RS28030 Genome accession   NZ_CP104727
Coordinates   6383026..6384729 (+) Length   567 a.a.
NCBI ID   WP_261746926.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain G5.80     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6378026..6389729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5P21_RS28015 (N5P21_28035) - 6378866..6380437 (-) 1572 WP_261746923.1 O-antigen ligase -
  N5P21_RS28020 (N5P21_28040) - 6380450..6382312 (-) 1863 WP_261746924.1 sulfatase-like hydrolase/transferase -
  N5P21_RS28025 (N5P21_28045) comP 6382406..6382804 (-) 399 WP_261746925.1 pilin Machinery gene
  N5P21_RS28030 (N5P21_28050) pilB 6383026..6384729 (+) 1704 WP_261746926.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5P21_RS28035 (N5P21_28055) - 6384733..6385951 (+) 1219 Protein_5551 type II secretion system F family protein -
  N5P21_RS28040 (N5P21_28060) pilD 6385955..6386827 (+) 873 WP_261746927.1 A24 family peptidase Machinery gene
  N5P21_RS28045 (N5P21_28065) coaE 6386901..6387509 (+) 609 WP_009617624.1 dephospho-CoA kinase -
  N5P21_RS28050 (N5P21_28070) yacG 6387506..6387715 (+) 210 WP_043272268.1 DNA gyrase inhibitor YacG -
  N5P21_RS28055 (N5P21_28075) - 6387990..6388184 (+) 195 WP_074977500.1 stability determinant -
  N5P21_RS28060 (N5P21_28080) - 6388244..6388435 (+) 192 WP_074977497.1 type II toxin-antitoxin system RelE/ParE family toxin -
  N5P21_RS28065 (N5P21_28085) - 6388442..6388657 (-) 216 WP_043318062.1 hypothetical protein -
  N5P21_RS28070 (N5P21_28090) - 6388765..6389481 (-) 717 WP_261746928.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62725.85 Da        Isoelectric Point: 5.7114

>NTDB_id=731603 N5P21_RS28030 WP_261746926.1 6383026..6384729(+) (pilB) [Pseudomonas citronellolis strain G5.80]
MNDQVVLSGLARQLVIAELLDEKSAQQAQQQAQRNKLPLVTYLVQNKLVKSRPLTELAAEQFGIAYCDLGSLDKDTIPRE
LISEKLVRQHRVIPLWRRGNKLFVGLSDPTNHQAITDVQFSTGLTTEAILVEDDKLGDTIEKLFESVTGGLDDLGDVDLE
GLDIESGDVERKEETIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDAQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPHCKKEHDVPSETLLHEGFP
EDRIGSFKLYAPVGCENCKHGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLVKAMHGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=731603 N5P21_RS28030 WP_261746926.1 6383026..6384729(+) (pilB) [Pseudomonas citronellolis strain G5.80]
ATGAATGATCAAGTCGTACTGTCCGGCCTCGCGCGACAACTGGTGATTGCTGAACTGCTCGATGAGAAGAGCGCACAGCA
GGCGCAGCAGCAGGCCCAGAGAAACAAGCTGCCACTTGTGACCTACCTGGTTCAGAACAAACTGGTGAAGAGCCGTCCGC
TGACCGAACTCGCCGCCGAGCAATTCGGCATCGCCTACTGCGATCTAGGCAGCCTGGACAAGGACACCATCCCACGGGAA
CTGATCAGCGAAAAACTCGTACGCCAGCACCGTGTCATTCCTCTCTGGCGCCGGGGCAACAAGCTTTTCGTCGGACTGTC
CGATCCAACCAACCACCAAGCCATCACTGACGTCCAGTTCAGCACCGGACTGACCACCGAGGCGATACTGGTCGAAGATG
ACAAGCTCGGTGACACCATTGAAAAGCTTTTCGAAAGTGTGACCGGCGGGCTTGACGACCTCGGCGATGTCGACCTGGAA
GGTCTAGATATCGAGAGCGGCGACGTCGAGCGCAAGGAAGAAACCATCGGCGGCGATGCGGACGATGCGCCAGTGGTGCG
CTTCGTCAATAAGATGCTGCTGGACGCCATCAAGGGCGGCTCGTCGGACTTGCACTTCGAGCCCTACGAAAAAGTCTACC
GAGTACGTTTTCGTACCGACGGCATGTTGCACGAGGTGGCCAAGCCGCCAATCCAATTGGCCAGCCGTATCTCCGCACGT
CTGAAGGTAATGGCCGGCCTGGACATTTCCGAACGGCGCAAACCCCAGGACGGCCGCATCAAAATGCGTGTCTCCAAGAG
CAAGTCCATTGATTTCCGCGTCAACACGCTGCCCACGCTATGGGGCGAAAAGATCGTAATGCGGATTCTCGATTCGGCCA
GCGCGCAGATGGGCATCGATGCCCTTGGCTACGAGGATGCGCAGAAGCAGCTCTACCTTGAGGCCCTGAAGCAACCGCAG
GGTATGATTCTGGTGACCGGTCCTACAGGCTCCGGCAAGACCGTATCCCTGTATACCGGCCTGAATATCCTCAATACCCC
GGACATCAATATCTCAACCGCCGAGGATCCGGTGGAGATCAACCTTGAGGGAATCAACCAGGTCAACGTCAACCCCCGCC
AGGGCATGGACTTCGCCCAGGCATTGCGCGCCTTCCTACGCCAGGATCCGGACGTAATAATGGTAGGAGAGATCCGCGAC
CTGGAAACCGCCGAGATTGCCATCAAGGCTGCACAAACAGGCCATATGGTGATGTCCACCTTGCACACCAACAGCGCTGC
GGAAACCCTGACGCGCCTGCTGAACATGGGCGTAGCCTCCTTCAACTTGGCCACCTCCACCAACCTGATCATCGCCCAGC
GCCTAGCGCGCAAGCTCTGCCCGCATTGCAAGAAGGAACACGACGTTCCTAGCGAAACCTTGCTTCACGAGGGATTCCCC
GAAGACAGGATCGGCAGCTTCAAGCTCTACGCCCCAGTGGGGTGTGAAAATTGCAAGCATGGCTACAAGGGTCGCGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGCAAAGAAGGCTTCAATGACCTGCGTACTTCGGGCCTAGTCAAGGCCATGCATGGCATTACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.172

100

0.584
  pilB Acinetobacter baumannii D1279779

57.522

99.647

0.573
  pilB Legionella pneumophila strain ERS1305867

54.674

100

0.547
  pilF Neisseria gonorrhoeae MS11

50.088

100

0.503
  pilB Vibrio cholerae strain A1552

50.266

99.295

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.381

95.767

0.492
  pilB Vibrio campbellii strain DS40M4

49.293

99.824

0.492
  pilF Thermus thermophilus HB27

38.313

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.298

0.369