Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N5P21_RS02860 Genome accession   NZ_CP104727
Coordinates   644764..646851 (+) Length   695 a.a.
NCBI ID   WP_074985595.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain G5.80     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 639764..651851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5P21_RS02840 (N5P21_02840) pilM 641870..642934 (+) 1065 WP_253391055.1 pilus assembly protein PilM Machinery gene
  N5P21_RS02845 (N5P21_02845) pilN 642934..643533 (+) 600 WP_009613648.1 type 4a pilus biogenesis protein PilN -
  N5P21_RS02850 (N5P21_02850) pilO 643530..644186 (+) 657 WP_261747450.1 type 4a pilus biogenesis protein PilO -
  N5P21_RS02855 (N5P21_02855) pilP 644183..644707 (+) 525 WP_074985598.1 type 4a pilus biogenesis lipoprotein PilP -
  N5P21_RS02860 (N5P21_02860) pilQ 644764..646851 (+) 2088 WP_074985595.1 type IV pilus secretin PilQ Machinery gene
  N5P21_RS02865 (N5P21_02865) aroK 646860..647378 (+) 519 WP_074985632.1 shikimate kinase AroK -
  N5P21_RS02870 (N5P21_02870) aroB 647430..648536 (+) 1107 WP_261747451.1 3-dehydroquinate synthase -
  N5P21_RS02875 (N5P21_02875) - 648543..650219 (+) 1677 WP_043268470.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74839.98 Da        Isoelectric Point: 5.2731

>NTDB_id=731586 N5P21_RS02860 WP_074985595.1 644764..646851(+) (pilQ) [Pseudomonas citronellolis strain G5.80]
MNRTMTRLGFALLAGLFAPSLLAANLQRVDVASLPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIINLTTLVPYSTRAQGNDLIILVGQGGGAPAAAASSAPSAAPAYAAAPRPAQPKPYVPVG
KAIRNIDFQRGEQGEGNVVIDLSDPSVSPDIQEQGGKIRLDFPRTQLPDSLRVRLDVKDFATPVQFVNASAAGDKASIVI
EPSGLYDYLVFQTDNRMTVSIKPMTSNEAEKRKKDSFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDIAKLFQSVT
GTSSGPGAQPAAGTDNRGSITVDDRTNAIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEASVDYTKELGVQWGGSL
SNRGNFNITGGANSTSNTFVDLGVTTATPTSGIGIGFVTDNTTLDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGSE
IPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMQVKVNKDEPDYQNALLGVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQTKTTSKVPFLGDVPVVGRLFKNDYVTDTKNELLVFLTPRIMNNQAIAIDR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=731586 N5P21_RS02860 WP_074985595.1 644764..646851(+) (pilQ) [Pseudomonas citronellolis strain G5.80]
ATGAACAGAACTATGACCCGCCTGGGCTTTGCCCTGTTGGCCGGCCTTTTCGCGCCGAGCCTGCTCGCCGCCAACCTGCA
GCGCGTCGATGTGGCCTCGCTGCCGGGGGATCGCGTGGAGCTCAAGCTGGGCTTCGACGAGCCGGTGGCCGCGCCGCGTG
GCTACACCATCGAGCAGCCGGCGCGCATTGCCCTGGACCTGCCGGGCGTGCAGAACAAACTGGGCAGCAAGAACCGCGAG
CTGGGCGTGGGCAACGCGCGCAGCCTGACCGTGGTCGAGGCCAAGGACCGCACCCGCCTGATCATCAACCTGACCACCCT
GGTGCCGTATTCCACCCGCGCCCAGGGCAACGACCTGATCATCCTGGTCGGCCAGGGTGGCGGCGCGCCCGCGGCGGCGG
CCAGCTCCGCGCCGAGCGCGGCCCCGGCCTATGCCGCGGCGCCTCGCCCGGCGCAGCCCAAGCCTTACGTGCCGGTGGGC
AAGGCGATCCGCAACATCGACTTCCAGCGTGGCGAGCAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCCAGCGT
CAGCCCGGACATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCCCGCGCACCCAGCTGCCCGACAGCCTGCGCGTGC
GCCTGGACGTGAAGGACTTCGCCACTCCGGTGCAGTTCGTCAACGCCTCGGCGGCCGGCGACAAGGCCAGCATCGTCATC
GAGCCCAGCGGCCTGTACGACTACCTGGTGTTCCAGACCGACAACCGCATGACCGTCAGCATCAAGCCGATGACCAGCAA
CGAGGCCGAGAAGCGCAAGAAGGACAGCTTCGCCTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GCTCGGTGCTGCAGCTGATCGCCGACTTCACCGACCTCAACCTGGTGGCCTCCGACACCGTGCAGGGCAACATCACCCTG
CGCCTGCAGAACGTGCCCTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAA
CGTCCTGCTGGTGGCACCGGCCGACGAGATCGCCGCGCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAAC
TGGCGCCCCTGCGTCGCGAACTGATCCAGGTGAACTACGCCAAGGCTTCCGACATCGCCAAGCTGTTCCAGTCCGTGACC
GGCACCAGTTCCGGCCCGGGCGCCCAGCCTGCCGCCGGCACCGACAACCGTGGCTCCATCACCGTCGACGACCGCACCAA
CGCCATCATCGCCTACCAGACCCAGGACCGCCTGGACGAGCTGCGCCGCATCGTCTCGCAGCTGGACATCCCGGTACGCC
AGGTGATGATCGAGGCGCGCATCGTCGAGGCCAGCGTCGACTACACCAAGGAGCTGGGGGTGCAATGGGGTGGTTCGCTG
AGCAATCGCGGCAACTTCAACATCACCGGTGGCGCCAACAGCACGAGCAATACCTTCGTTGACCTGGGCGTGACCACCGC
GACGCCGACCTCCGGCATCGGCATCGGCTTCGTCACCGACAACACCACCCTGGACCTGCAGCTCAACGCCATGGAGAAGA
CCGGCAACGGCGAAGTGGTGTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAG
ATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCCACCTCGGTGTCCTTCAAGGAGGCTGCGCTGTCCCTGGAGGTCACTCC
GCAGATCACCCCGGACAACCGCATCATCATGCAGGTCAAGGTGAACAAGGACGAGCCCGACTACCAGAACGCGCTGCTCG
GCGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGTGAAACCATCGTCATCGGCGGGGTG
TTCTCCAACACCCAGACCAAGACCACCTCCAAGGTCCCGTTCCTCGGCGACGTACCGGTCGTCGGCCGCCTGTTCAAGAA
CGACTATGTGACGGACACCAAGAACGAGCTTCTGGTTTTCCTCACTCCCCGGATCATGAATAATCAGGCCATTGCCATCG
ATCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-454)

AMIN domain (AMIN)

(40-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.178

100

0.803
  comQ Acinetobacter baylyi ADP1

42.037

100

0.422
  pilQ Acinetobacter baumannii strain A118

40.336

100

0.414
  pilQ Acinetobacter baumannii D1279779

40.336

100

0.414
  pilQ Legionella pneumophila strain Lp02

39.092

98.273

0.384
  pilQ Legionella pneumophila strain ERS1305867

38.946

98.273

0.383