Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GZN30_RS14200 Genome accession   NZ_AP019657
Coordinates   3146215..3147906 (+) Length   563 a.a.
NCBI ID   WP_075651892.1    Uniprot ID   -
Organism   Vibrio ponticus strain DSM 16217     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3141215..3152906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZN30_RS14180 pilM 3143448..3144434 (+) 987 WP_075651858.1 type IV pilus assembly protein PilM -
  GZN30_RS14185 - 3144424..3145026 (+) 603 WP_139312273.1 PilN domain-containing protein -
  GZN30_RS14190 - 3145013..3145624 (+) 612 WP_075651862.1 type 4a pilus biogenesis protein PilO -
  GZN30_RS14195 - 3145631..3146200 (+) 570 WP_075651864.1 pilus assembly protein PilP -
  GZN30_RS14200 pilQ 3146215..3147906 (+) 1692 WP_075651892.1 type IV pilus secretin PilQ Machinery gene
  GZN30_RS14205 aroK 3148071..3148589 (+) 519 WP_075651866.1 shikimate kinase AroK -
  GZN30_RS14210 aroB 3148620..3149708 (+) 1089 WP_075651868.1 3-dehydroquinate synthase -
  GZN30_RS14215 - 3149763..3151241 (+) 1479 WP_075651870.1 AAA family ATPase -
  GZN30_RS14220 - 3151375..3152199 (+) 825 WP_075651872.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61781.45 Da        Isoelectric Point: 4.5976

>NTDB_id=73136 GZN30_RS14200 WP_075651892.1 3146215..3147906(+) (pilQ) [Vibrio ponticus strain DSM 16217]
MRFRLFAALMMVTLMHTTTSLAQVTSNRLEGIDFQLNKQRNSKIMLELSEPHIALDIEKTAQGLSIKVFDTAIDAEQLKR
FDVADFASSVQMLELFQHGDDIELTAQIEGAFRYHYQLNDHLLEVIVQSVEPNKGEELSLPAKNMSINFQDVPVRNVLQL
IAQYNGFNLVMADSVSGNISLRLDEVPWTQILDVILQVKGLAKHVDGNVVIVAPLAEIEQREQIRLEQARLESERVGFQS
QIIKVNFANASDIAELVGGEGVTGMLSERGTVRVDERTNSLLIRDFSTNIDAVREIIAALDVPIKQVQIEARIVTISEGN
LDELGVRWSVNSNDGRLGASLEGNFDQRNQSQLLDANGSLLPDVDISELLNVNLAAAAANSSTIAFQVAKLGSSTLLDLE
LSALQRESKAEVISSPRLITTNKQPAYIEQGTEIPYLEAASSGATSVSFKKAVLSLKVTPQITPDNRLILDLNVTQDRPG
EVLKTGTGEAVSINTQRIGTQVLVENGETIVLGGIYQRSVTDTVDKVPLLGDLPLLGALFRRSYQQLGKSELLIFVTPKV
VVH

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=73136 GZN30_RS14200 WP_075651892.1 3146215..3147906(+) (pilQ) [Vibrio ponticus strain DSM 16217]
ATGCGTTTTAGGTTATTTGCTGCACTGATGATGGTTACATTGATGCACACGACAACAAGCTTAGCGCAAGTGACGTCTAA
CCGCTTAGAAGGGATTGATTTTCAACTTAATAAGCAGCGCAACAGTAAAATTATGCTTGAGTTGAGTGAGCCGCATATTG
CATTGGATATCGAAAAAACGGCTCAAGGTTTATCAATTAAAGTCTTTGATACGGCGATTGATGCTGAGCAACTTAAGCGC
TTTGATGTCGCTGATTTTGCCAGTAGCGTGCAGATGCTTGAGCTGTTTCAGCATGGTGATGATATTGAGTTAACCGCACA
AATTGAGGGAGCGTTTCGCTATCACTATCAACTCAATGATCATTTGCTGGAAGTGATAGTGCAGTCTGTTGAGCCAAATA
AAGGCGAAGAGTTAAGCTTACCCGCAAAGAATATGTCGATTAACTTTCAAGACGTGCCGGTGCGTAACGTGTTGCAACTT
ATCGCGCAATACAATGGCTTTAACTTAGTCATGGCAGACTCGGTGAGCGGTAATATCAGTTTGCGGTTAGATGAAGTGCC
GTGGACGCAAATACTCGATGTGATTTTACAAGTGAAAGGGCTGGCTAAACATGTTGACGGTAATGTCGTGATTGTGGCGC
CCTTGGCGGAGATTGAACAAAGGGAGCAGATTCGTCTTGAACAGGCGCGTTTAGAGAGTGAGCGCGTTGGGTTTCAATCG
CAAATCATTAAAGTTAACTTTGCCAATGCAAGTGATATTGCAGAGCTAGTCGGGGGAGAAGGCGTCACGGGTATGCTTTC
AGAGCGCGGAACGGTGCGGGTCGACGAACGTACCAATTCCCTCTTGATTCGTGATTTTTCGACTAATATTGATGCTGTGC
GAGAGATTATTGCCGCTTTGGATGTGCCAATTAAACAGGTGCAGATCGAGGCGCGGATTGTCACCATCAGTGAAGGAAAT
CTCGATGAGTTAGGTGTACGTTGGAGTGTCAACTCCAATGATGGTAGACTAGGCGCCTCTTTAGAGGGTAACTTTGACCA
GCGCAATCAATCTCAACTCCTTGACGCTAATGGTAGCTTGCTTCCTGACGTTGATATTAGTGAGTTGCTCAATGTAAATT
TAGCCGCTGCGGCAGCGAATTCTTCGACCATCGCTTTTCAAGTAGCTAAACTAGGTAGCAGTACTTTACTCGATTTGGAA
CTGTCGGCATTGCAGCGTGAGTCGAAGGCGGAAGTGATTTCCAGCCCACGTCTTATCACGACTAATAAGCAACCTGCTTA
TATTGAGCAGGGGACAGAAATTCCATATTTGGAAGCTGCATCGAGTGGTGCAACATCAGTATCCTTTAAAAAGGCGGTAT
TAAGCCTTAAAGTAACGCCGCAAATTACCCCTGATAATCGATTAATTTTAGATTTAAATGTCACTCAAGATAGACCGGGT
GAGGTGTTGAAAACGGGTACCGGAGAAGCGGTCTCAATCAACACTCAACGTATTGGCACGCAAGTGTTAGTAGAAAATGG
CGAAACCATAGTATTGGGCGGTATTTATCAGCGCAGTGTGACTGATACAGTGGATAAAGTACCACTGCTCGGTGACTTGC
CGCTGCTTGGCGCTTTGTTTCGGCGTAGCTACCAACAATTGGGTAAAAGTGAGCTACTTATTTTCGTTACGCCAAAAGTA
GTTGTGCATTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(403-561)

Bacterial type II/III secretion system short domain (Secretin_N)

(241-306)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

60.87

98.046

0.597
  pilQ Vibrio cholerae strain A1552

60.87

98.046

0.597
  pilQ Vibrio campbellii strain DS40M4

61.426

97.158

0.597
  pilQ Legionella pneumophila strain Lp02

39.633

96.803

0.384
  pilQ Legionella pneumophila strain ERS1305867

39.45

96.803

0.382
  pilQ Pseudomonas aeruginosa PAK

37.568

97.869

0.368
  comQ Acinetobacter baylyi ADP1

36.348

100

0.364

Multiple sequence alignment