Detailed information NTG0731235

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: N5980_RS01885
• Genome accession: NZ_CP104651
• Coordinates: 402084..403142 (+)
• Length: 352 a.a.
• NCBI ID: WP_004787132.1
• Uniprot ID: N8VCV1
• Organism: Acinetobacter variabilis strain 2021CK-01005
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 397084..408142

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5980_RS01870 (N5980_01870)	mraY	397350..398468 (+)	1119	WP_175966246.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
N5980_RS01875 (N5980_01875)	-	398527..399330 (+)	804	WP_175966244.1	putative RNA methyltransferase	-
N5980_RS01880 (N5980_01880)	ponA	399389..401917 (-)	2529	WP_175966243.1	penicillin-binding protein PBP1a	-
N5980_RS01885 (N5980_01885)	pilM	402084..403142 (+)	1059	WP_004787132.1	pilus assembly protein PilM	Machinery gene
N5980_RS01890 (N5980_01890)	comN	403142..403780 (+)	639	WP_004787129.1	PilN domain-containing protein	Machinery gene
N5980_RS01895 (N5980_01895)	comO	403777..404493 (+)	717	WP_175966241.1	type 4a pilus biogenesis protein PilO	Machinery gene
N5980_RS01900 (N5980_01900)	pilP	404493..405020 (+)	528	WP_034169727.1	pilus assembly protein PilP	Machinery gene
N5980_RS01905 (N5980_01905)	pilQ	405037..407202 (+)	2166	WP_175966239.1	type IV pilus secretin PilQ family protein	Machinery gene
N5980_RS01910 (N5980_01910)	aroK	407239..407775 (+)	537	WP_175966238.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39059.59 Da
• Isoelectric Point: 4.5754

>NTDB_id=731235 N5980_RS01885 WP_004787132.1 402084..403142(+) (pilM) [Acinetobacter variabilis strain 2021CK-01005]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKNGRYWVESYALVPLPESSVVEKNILNPEAVGDALGRAINLANTQSSQSAF
AIPTSMVITKIIEMDADMSDDEREVQIREDAEQYIPFPLDEASLDFEVLPDRLTNPNRVNVLLVATRIENVEARSEVLQI
ANLNPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=731235 N5980_RS01885 WP_004787132.1 402084..403142(+) (pilM) [Acinetobacter variabilis strain 2021CK-01005]
GTGCGCAGGTTATATCGTAAGCCAAATAAGGGGTTAATAGGAGTCGATATTAGTTCGACTTCTGTTAAAGTTTTAGAGCT
CTCTGTAAAGAACGGTCGGTACTGGGTAGAAAGTTATGCTTTGGTCCCACTACCAGAAAGTAGTGTCGTTGAAAAAAACA
TTTTAAATCCAGAAGCGGTGGGTGATGCGTTGGGGCGTGCGATTAATTTGGCCAATACCCAGTCCAGTCAGTCAGCTTTT
GCGATTCCAACTTCTATGGTCATCACCAAAATCATCGAAATGGATGCCGATATGTCGGATGATGAACGTGAAGTTCAGAT
TCGTGAAGATGCTGAACAATATATTCCTTTCCCACTTGACGAAGCCAGCCTGGACTTCGAAGTTCTGCCAGACCGTTTAA
CCAATCCGAACCGGGTCAATGTCCTGTTGGTAGCGACCCGAATAGAAAATGTTGAAGCGCGTTCTGAAGTACTGCAAATT
GCCAATCTTAATCCAAAAATTGCAGATGTCGAAAGTTTTGCATTAGAAAATGCATTCAAGGTGTTTTCGGATACCTTACC
GATGGGGGTAAATACTGTCGGGATCCTGGATATTGGTCATAGTATGACGACCTTATCCGTCATGCAGAATAACAAGATCA
TTTATACCCGTGAGCAGGTTTTTGGTGGCAAACAGCTGACTCAGGAAATCCAGAACCGTTATGGATTGTCTTTTGAGGAA
GCAGGTCGTGCTAAAAAAACCCGTGCCTTACCGGATGACTATGACATCGAAGTGCTGGAACCTTTTCTAGATGCAGTCGT
GCAGCAGGCAGCGCGTTCATTACAGTTCTTCTTCTCTTCATCTCAGTTTAACGAGATTGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGTCTGGCAAAACTATTACAGCAAAAACTCGGCTACCGGGTCACGATTGCCAATCCGTTCCTG
CAAATGGGCTTTTCTCCTCAAATTGATATTAAAAAAATTGAAAATGACGCGTCCTCACTCATGGTTGCATGTGGTTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8VCV1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.511	100	0.855
comM	Acinetobacter nosocomialis M2	85.511	100	0.855
comM	Acinetobacter baylyi ADP1	78.977	100	0.79
pilM	Legionella pneumophila strain ERS1305867	39.831	100	0.401