Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GZK95_RS12835 Genome accession   NZ_AP019654
Coordinates   2820564..2822243 (+) Length   559 a.a.
NCBI ID   WP_075715364.1    Uniprot ID   -
Organism   Vibrio panuliri strain JCM 19500     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2815564..2827243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZK95_RS12820 pncC 2816546..2817037 (-) 492 WP_075715366.1 nicotinamide-nucleotide amidase -
  GZK95_RS12825 mutS 2817341..2819917 (+) 2577 WP_075715365.1 DNA mismatch repair protein MutS -
  GZK95_RS12830 - 2820148..2820564 (+) 417 WP_075706953.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GZK95_RS12835 pilB 2820564..2822243 (+) 1680 WP_075715364.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GZK95_RS12840 - 2822245..2823048 (+) 804 WP_083626249.1 type II secretion system F family protein -
  GZK95_RS12845 pilC 2823045..2823467 (+) 423 WP_083626247.1 type II secretion system F family protein Machinery gene
  GZK95_RS12850 pilD 2823515..2824384 (+) 870 WP_075715363.1 A24 family peptidase Machinery gene
  GZK95_RS12855 coaE 2824386..2824994 (+) 609 WP_075706961.1 dephospho-CoA kinase -
  GZK95_RS12860 zapD 2825028..2825768 (+) 741 WP_075706963.1 cell division protein ZapD -
  GZK95_RS12865 yacG 2825823..2826017 (+) 195 WP_075706965.1 DNA gyrase inhibitor YacG -
  GZK95_RS12870 rplS 2826341..2826694 (-) 354 WP_038173824.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62750.98 Da        Isoelectric Point: 6.5021

>NTDB_id=73095 GZK95_RS12835 WP_075715364.1 2820564..2822243(+) (pilB) [Vibrio panuliri strain JCM 19500]
MPLLEMLLRERYLTSTQLASLPIEQHTSLCETLVNSGTLSHQQLSIAMAQLFNLEVVEIEHFDYQSLCRTLGLRELITRY
QAVPIKRNTQQLTLAIFDPSQTGLEQEFGFATHLQVVLVIADIRAIKKAINNLYGRNNPHINTYHKEIDTTELEQMVDLS
QQEQNSLDELTQDDAPISRYIHQVLLDAIRKGASDIHFEPYEEHFRIRFRCDGLLLEAHHPPVSLGRRLTTRVKILAQLN
IAERRLPQDGRTKLKLNDALSADMRISTLPTLWGEKVVLRLLNNTPSVLNIDKLGFTQEQQQTYVQTLKRPQGLIMITGP
TGSGKTVTLYAGLATLNTPQRNIATAEDPIEMNLFGINQVQVQPQIGFDFSTALRTFLRQDPDVIMVGEIRDFETAEIAV
KAAQTGHLVLSTLHTNSAAETLVRLNNMGIQPYNLAASLSLVIAQRLVRRLCPLCKQNAELTLEQASYLSVSHPQAVYCA
NHSGCEHCNQGYLGRIGLFEILPINQEIKRKILAQSNASELQQQAVAQGMQTIKSCATEKLLQGETSLAELERVLLFGE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=73095 GZK95_RS12835 WP_075715364.1 2820564..2822243(+) (pilB) [Vibrio panuliri strain JCM 19500]
ATGCCCTTGCTTGAGATGCTACTGAGAGAGCGCTACCTTACCTCAACCCAACTTGCATCCTTGCCGATTGAACAACACAC
ATCTCTGTGCGAGACATTGGTAAACTCTGGCACATTAAGCCATCAGCAACTTAGTATCGCCATGGCTCAACTCTTTAACC
TTGAAGTGGTTGAGATCGAACACTTTGACTATCAATCCTTATGTCGCACTTTGGGATTAAGAGAGCTGATTACCCGTTAC
CAAGCTGTTCCGATAAAGAGAAATACTCAACAGCTCACTCTTGCCATTTTTGACCCAAGCCAAACCGGCTTGGAGCAAGA
GTTTGGCTTTGCCACCCACCTTCAAGTTGTTTTGGTTATTGCGGATATTCGTGCAATTAAGAAAGCAATCAATAATTTAT
ATGGCCGCAATAATCCCCATATCAATACATACCATAAGGAGATTGATACGACTGAACTCGAGCAAATGGTCGATCTTTCT
CAACAAGAGCAAAACTCTCTCGACGAGTTAACCCAAGATGATGCTCCTATCAGCCGCTATATCCATCAGGTCCTACTCGA
TGCAATCCGTAAAGGGGCTTCCGATATTCATTTCGAACCATACGAAGAACATTTTCGAATTCGTTTTCGTTGCGATGGCC
TCCTACTTGAAGCACACCACCCTCCAGTTTCGTTAGGGCGAAGATTAACAACACGAGTAAAAATCCTCGCTCAATTAAAC
ATCGCGGAAAGGCGCTTGCCACAAGATGGACGCACCAAGCTAAAACTCAACGACGCCTTGTCGGCAGATATGCGTATATC
CACTCTGCCAACATTATGGGGCGAAAAGGTGGTGCTGCGTTTACTGAACAATACACCAAGCGTACTCAACATCGACAAAC
TTGGATTTACTCAAGAACAACAACAGACTTACGTTCAAACGTTGAAGCGCCCGCAAGGACTCATCATGATCACCGGACCG
ACTGGCAGTGGTAAAACAGTCACTCTTTATGCAGGTCTCGCTACACTTAACACCCCTCAACGTAATATTGCGACCGCCGA
AGATCCAATCGAAATGAATCTATTTGGCATAAACCAAGTGCAAGTCCAACCCCAAATTGGCTTTGATTTCTCCACGGCGC
TACGCACATTTTTACGCCAAGACCCAGACGTGATAATGGTCGGAGAAATACGTGATTTTGAAACCGCTGAAATTGCCGTC
AAAGCAGCACAAACGGGTCACTTAGTACTTTCGACACTACACACTAACTCTGCGGCTGAAACGCTAGTTCGACTGAATAA
CATGGGGATTCAACCATACAATCTCGCGGCTTCATTGAGCTTAGTGATAGCGCAACGCTTGGTTCGCCGTTTGTGTCCGT
TATGCAAACAGAACGCCGAGCTCACTTTAGAGCAAGCCAGTTATCTCTCAGTCTCGCATCCTCAAGCCGTATATTGTGCC
AACCACTCAGGCTGTGAACATTGTAATCAAGGCTACTTAGGCCGAATTGGTCTATTTGAAATCCTACCAATCAATCAAGA
AATCAAACGCAAAATTCTCGCTCAATCTAATGCTTCGGAACTGCAACAGCAGGCAGTAGCACAGGGGATGCAAACTATAA
AATCCTGTGCGACCGAGAAGCTACTTCAAGGCGAGACGAGCTTGGCTGAACTTGAGCGTGTTTTACTATTTGGGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(183-450)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(33-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

54.383

100

0.544
  pilB Vibrio cholerae strain A1552

56.309

94.991

0.535
  pilB Vibrio campbellii strain DS40M4

54.151

94.812

0.513
  pilB Legionella pneumophila strain ERS1305867

44.248

100

0.447
  pilB Acinetobacter baumannii D1279779

42.857

95.17

0.408
  pilB Acinetobacter baylyi ADP1

42.776

94.097

0.403
  pilF Neisseria gonorrhoeae MS11

41.143

93.918

0.386
  pilF Thermus thermophilus HB27

38.041

94.991

0.361

Multiple sequence alignment