Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N5O87_RS04215 Genome accession   NZ_CP104582
Coordinates   875691..876908 (-) Length   405 a.a.
NCBI ID   WP_108234286.1    Uniprot ID   A0A427HNM1
Organism   Pseudomonas sp. GD03919     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 870691..881908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O87_RS04185 (N5O87_04185) - 870982..871215 (-) 234 WP_147810319.1 hypothetical protein -
  N5O87_RS04190 (N5O87_04190) - 871344..872033 (-) 690 WP_279532178.1 lipopolysaccharide kinase InaA family protein -
  N5O87_RS04195 (N5O87_04195) - 872279..872596 (+) 318 WP_279532179.1 IS66-like element accessory protein TnpA -
  N5O87_RS04200 (N5O87_04200) tnpB 872602..872928 (+) 327 WP_279531102.1 IS66 family insertion sequence element accessory protein TnpB -
  N5O87_RS04205 (N5O87_04205) - 872990..874525 (+) 1536 WP_279532180.1 IS66 family transposase -
  N5O87_RS04210 (N5O87_04210) pilD 874821..875690 (-) 870 WP_279532181.1 prepilin peptidase Machinery gene
  N5O87_RS04215 (N5O87_04215) pilC 875691..876908 (-) 1218 WP_108234286.1 type II secretion system F family protein Machinery gene
  N5O87_RS04220 (N5O87_04220) pilB 876911..878614 (-) 1704 WP_279532182.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5O87_RS04225 (N5O87_04225) - 878836..879282 (+) 447 WP_279532183.1 pilin -
  N5O87_RS04230 (N5O87_04230) - 880176..880706 (+) 531 WP_279532184.1 REP-associated tyrosine transposase -
  N5O87_RS04235 (N5O87_04235) - 880769..881113 (-) 345 WP_004424987.1 type II toxin-antitoxin system HicB family antitoxin -
  N5O87_RS04240 (N5O87_04240) - 881110..881463 (-) 354 WP_004424984.1 type II toxin-antitoxin system HicA family toxin -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44007.75 Da        Isoelectric Point: 9.7598

>NTDB_id=730702 N5O87_RS04215 WP_108234286.1 875691..876908(-) (pilC) [Pseudomonas sp. GD03919]
MAEKALKTSVFTWEGKNKSGAVVKGELSGQNPSLVKAQLRKQGINPTKVRKKGVSLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIISEGFDNPNMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVESGEQAGALENLLDRIATYKEKT
EALKAKIKKAMNYPIAVVVVAIIVSAILLIKVVPQFEEVFANFGAELPAFTLMVIGISQFLQEWWFIFLIAIIAAAFAFK
EAMKRSQKLRDSVDRGVLKLPIVGDILYKSAVARFARTLSTTFAAGVPLVDALDSVSGATGNVVFKNATNKIKADVSTGM
QLNFSMRTTGTFPSMAVQMTAIGEESGSLDEMLDKVASFYEAEVDNMVDGLTSLMEPIIMSVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=730702 N5O87_RS04215 WP_108234286.1 875691..876908(-) (pilC) [Pseudomonas sp. GD03919]
ATGGCGGAAAAAGCTCTAAAAACCAGTGTGTTCACCTGGGAAGGCAAGAACAAAAGCGGTGCCGTCGTCAAGGGGGAGCT
CAGCGGGCAGAACCCGTCCTTGGTCAAGGCGCAACTGCGCAAACAGGGGATCAATCCGACCAAAGTACGCAAAAAGGGGG
TGTCGCTCTTCAGCGCAGGCAAGAAGATCAAACCCATGGATATCGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAA
GCTGGCGTGCCACTGCTGCAGTCCTTCGACATCATCTCAGAGGGCTTCGACAACCCCAACATGCGCAAGCTGGTCGACGA
GGTGAAGCAGGAGGTGGCTGCTGGTAACAGTTTTGCCGCCTCGCTGCGCAAGAAACCACAGTACTTCGATGAACTGTATT
GCAACCTGGTTGAGTCTGGCGAACAGGCCGGCGCGCTGGAGAATCTGCTGGACCGAATCGCCACCTACAAAGAAAAGACC
GAAGCGCTGAAGGCCAAGATCAAGAAGGCGATGAACTATCCAATCGCTGTTGTGGTGGTCGCCATCATCGTCTCAGCCAT
TTTGCTCATTAAAGTTGTACCGCAATTCGAGGAAGTTTTCGCCAACTTCGGCGCTGAACTACCTGCCTTTACGCTGATGG
TAATAGGCATATCGCAATTTCTTCAGGAGTGGTGGTTCATTTTTCTGATTGCAATCATCGCCGCTGCCTTTGCATTCAAA
GAAGCGATGAAGCGCTCGCAGAAACTGCGCGACTCGGTTGACCGTGGCGTACTGAAACTGCCAATCGTCGGCGACATTCT
TTACAAATCTGCGGTAGCCCGCTTCGCACGCACACTATCAACGACTTTTGCCGCTGGCGTGCCACTGGTCGATGCGCTGG
ATTCGGTCTCCGGCGCCACCGGTAATGTGGTGTTCAAGAATGCGACCAACAAGATCAAAGCAGATGTATCTACCGGCATG
CAGCTTAACTTCTCAATGCGCACCACCGGCACCTTCCCCTCCATGGCCGTGCAGATGACCGCTATTGGCGAAGAGTCTGG
CTCGCTGGATGAGATGCTCGATAAGGTAGCGAGCTTCTATGAAGCAGAAGTGGACAACATGGTTGACGGCCTGACCAGCC
TGATGGAGCCCATCATCATGTCCGTGCTGGGCGTTCTGGTCGGTGGCCTGATCATTGCGATGTACCTTCCAATCTTCCAG
CTGGGCGCCGTAGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A427HNM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.716

100

0.827
  pilC Acinetobacter baylyi ADP1

60.099

100

0.602
  pilC Acinetobacter baumannii D1279779

59.753

100

0.598
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria gonorrhoeae MS11

45.522

99.259

0.452
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

40.657

97.778

0.398