Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N5O83_RS02290 Genome accession   NZ_CP104579
Coordinates   485067..487187 (+) Length   706 a.a.
NCBI ID   WP_279534276.1    Uniprot ID   A0AA42Q9J8
Organism   Pseudomonas oleovorans strain GD04132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480067..492187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O83_RS02270 (N5O83_02275) pilM 482234..483298 (+) 1065 WP_074855141.1 pilus assembly protein PilM Machinery gene
  N5O83_RS02275 (N5O83_02280) pilN 483298..483870 (+) 573 WP_003459024.1 type 4a pilus biogenesis protein PilN -
  N5O83_RS02280 (N5O83_02285) pilO 483867..484490 (+) 624 WP_003459026.1 type 4a pilus biogenesis protein PilO -
  N5O83_RS02285 (N5O83_02290) pilP 484487..485011 (+) 525 WP_003459027.1 type 4a pilus biogenesis lipoprotein PilP -
  N5O83_RS02290 (N5O83_02295) pilQ 485067..487187 (+) 2121 WP_279534276.1 type IV pilus secretin PilQ Machinery gene
  N5O83_RS02295 (N5O83_02300) aroK 487193..487711 (+) 519 WP_084341405.1 shikimate kinase AroK -
  N5O83_RS02300 (N5O83_02305) aroB 487763..488866 (+) 1104 WP_104730115.1 3-dehydroquinate synthase -
  N5O83_RS02305 (N5O83_02310) - 488877..490460 (+) 1584 WP_074855137.1 AAA family ATPase -

Sequence

Protein


Download         Length: 706 a.a.        Molecular weight: 76170.33 Da        Isoelectric Point: 4.8011

>NTDB_id=730617 N5O83_RS02290 WP_279534276.1 485067..487187(+) (pilQ) [Pseudomonas oleovorans strain GD04132]
MNSSLSRLSVALLAALLSPAVLAANLQDLNVASLPGDRVELKLSFDEPVTAPRGYTIEQPARIALDLPGVSNKLGSKNRE
LGVGNARSVTIVEAKDRTRLIVNLTSLAPYSTRVEGNDLYVLVGDSSAVASRPSASVPASVAAVPPKKTYGPQAKAISNI
DFQRGEQGEGNVVITLSDASISPDIQEQGGKIRLDFAKTDLPESLRVRLDVKDFATPVQFVSATGSADRTSIVIEPVGLY
DYLAYQTENRLTLSVKPLTQDDVERRKAERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVP
WDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADMAKLFQSVTSADGAS
SDARGSVTVDDRTNSIIAYQTQDRLDELRRIIAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGATRRGNWSVYGKDGA
TSFDNESGQVYLPGTDTVGNVSLQDGVAPVPFVDMGVLGSTSGIGIGFLTDNVTLDLQLSAMEKTGNGEIVSQPKVVTSD
KETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFARALNGVPPINKNEVNAKVLV
NDGETIVIGGVFSNTQTKGVEKVPFLGDLPFLGRVFRRDIVSDNKAELLVFITPRIMNNQAIAVNR

Nucleotide


Download         Length: 2121 bp        

>NTDB_id=730617 N5O83_RS02290 WP_279534276.1 485067..487187(+) (pilQ) [Pseudomonas oleovorans strain GD04132]
ATGAACAGCTCTCTTTCGCGTCTTAGCGTCGCTCTGCTGGCGGCGCTGCTGTCGCCCGCGGTATTGGCGGCCAATCTTCA
GGATTTGAATGTCGCCAGTTTGCCTGGTGATCGAGTTGAGCTGAAGCTTTCCTTCGATGAGCCGGTAACGGCTCCTCGCG
GCTATACCATCGAGCAACCCGCGCGAATCGCTCTGGACCTGCCGGGAGTGTCGAACAAGCTCGGCTCCAAGAATCGTGAA
CTGGGTGTCGGTAATGCGCGTAGCGTTACCATTGTCGAGGCCAAGGACCGTACCCGTCTGATTGTCAATCTCACCAGTCT
GGCGCCATACAGCACTCGAGTTGAGGGTAATGATCTGTATGTCCTGGTTGGAGATTCTTCTGCCGTCGCGTCGCGGCCCT
CAGCCTCGGTGCCTGCTTCGGTAGCGGCGGTTCCGCCCAAGAAAACCTACGGTCCTCAAGCGAAAGCAATCAGTAATATC
GACTTTCAGCGTGGGGAGCAGGGTGAGGGTAATGTCGTCATTACCCTTTCCGATGCATCGATCAGTCCTGATATCCAAGA
GCAGGGTGGGAAGATTCGTCTGGACTTCGCCAAGACTGACCTGCCCGAGTCGCTGCGTGTACGGCTGGATGTGAAGGATT
TCGCCACGCCGGTGCAGTTCGTCAGTGCTACGGGTAGCGCAGACAGAACCAGTATCGTGATTGAACCAGTCGGGTTATAC
GATTACCTGGCTTACCAGACCGAGAACAGGCTGACGTTGAGTGTCAAACCTCTCACTCAGGATGACGTAGAACGCCGCAA
GGCTGAGCGCTTTGCTTATACCGGGGAGAAATTGTCGCTCAACTTTCAGGATATCGACGTGCGTTCGGTGCTGCAGCTGA
TTGCGGACTTCACCGATCTGAACCTGGTTGCAAGCGATACTGTCGCCGGCAATATCACTCTGCGTTTGCAGAACGTGCCA
TGGGATCAGGCGTTGGACCTGGTGCTGAAGACCAAGGGGCTGGATAAGCGCCAGGTTGGTAATGTATTGCTCGTTGCTCC
GGCAGATGAGATCGCCGCTCGTGAGCGTCAGGAGTTGGAGTCGCAAAAGCAAATTGCTGAGCTGGCTCCGCTGCGTCGTG
AACTGATTCAGGTGAATTATGCCAAGGCTGCTGATATGGCGAAGCTGTTTCAGTCGGTCACCAGTGCTGATGGTGCATCA
TCCGATGCCCGTGGTTCTGTAACGGTTGATGATCGCACTAACAGCATTATTGCTTACCAGACGCAGGATCGCCTGGATGA
GTTGCGGCGTATCATTGCCCAGCTCGACATTCCTGTGCGTCAGGTAATGATCGAGGCGCGTATCGTCGAGGCCAACGTGG
ACTACGACAAGGCCTTGGGTGTGCGTTGGGGAGGGGCAACTCGTCGCGGAAACTGGAGCGTCTACGGCAAGGACGGTGCA
ACTTCCTTCGATAATGAAAGCGGGCAGGTCTATTTGCCGGGCACCGATACGGTAGGCAACGTTTCGCTGCAGGATGGTGT
GGCTCCTGTGCCATTCGTCGACATGGGGGTTTTGGGCAGTACTTCAGGTATAGGCATCGGCTTCCTTACCGACAATGTGA
CACTGGATCTACAGCTGTCGGCCATGGAGAAGACCGGTAATGGCGAGATTGTCTCCCAGCCCAAGGTTGTTACTTCGGAC
AAGGAGACAGCGAAAATCTTGAAGGGGCAGGAAGTGCCCTATCAGGAAGCCAGCTCTAGTGGCGCGACCAGTACATCCTT
CAAGGAGGCGGCTCTCTCCCTAGAGGTTACTCCTCAGATTACTCCGGATAATCGCATCATCATGGAAGTGAAAGTAACCA
AGGATGCGCCAGACTTCGCCCGGGCCCTGAACGGGGTGCCGCCGATCAACAAGAACGAAGTCAATGCCAAGGTACTGGTC
AATGATGGCGAGACCATTGTGATCGGTGGGGTGTTCTCCAACACCCAGACCAAGGGTGTAGAGAAAGTCCCATTCCTTGG
TGACCTGCCATTCTTGGGCCGAGTGTTCCGTCGTGACATCGTCAGCGACAACAAGGCCGAGTTGCTGGTTTTCATCACTC
CGCGCATCATGAACAACCAAGCCATTGCCGTGAACCGATGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)

Bacterial type II and III secretion system protein (Secretin)

(541-697)

AMIN domain (AMIN)

(37-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.731

100

0.786
  pilQ Acinetobacter baumannii strain A118

40.108

100

0.419
  pilQ Acinetobacter baumannii D1279779

40.108

100

0.419
  comQ Acinetobacter baylyi ADP1

41.655

99.292

0.414
  pilQ Legionella pneumophila strain ERS1305867

38.496

96.034

0.37
  pilQ Legionella pneumophila strain Lp02

38.348

96.034

0.368