Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   N5O83_RS02270 Genome accession   NZ_CP104579
Coordinates   482234..483298 (+) Length   354 a.a.
NCBI ID   WP_074855141.1    Uniprot ID   A0A2S7FHW1
Organism   Pseudomonas oleovorans strain GD04132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477234..488298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O83_RS02260 (N5O83_02265) - 478283..479551 (+) 1269 WP_074855142.1 malic enzyme-like NAD(P)-binding protein -
  N5O83_RS02265 (N5O83_02270) - 479641..482052 (-) 2412 WP_279534275.1 penicillin-binding protein 1A -
  N5O83_RS02270 (N5O83_02275) pilM 482234..483298 (+) 1065 WP_074855141.1 pilus assembly protein PilM Machinery gene
  N5O83_RS02275 (N5O83_02280) pilN 483298..483870 (+) 573 WP_003459024.1 type 4a pilus biogenesis protein PilN -
  N5O83_RS02280 (N5O83_02285) pilO 483867..484490 (+) 624 WP_003459026.1 type 4a pilus biogenesis protein PilO -
  N5O83_RS02285 (N5O83_02290) pilP 484487..485011 (+) 525 WP_003459027.1 type 4a pilus biogenesis lipoprotein PilP -
  N5O83_RS02290 (N5O83_02295) pilQ 485067..487187 (+) 2121 WP_279534276.1 type IV pilus secretin PilQ Machinery gene
  N5O83_RS02295 (N5O83_02300) aroK 487193..487711 (+) 519 WP_084341405.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37839.28 Da        Isoelectric Point: 4.4647

>NTDB_id=730616 N5O83_RS02270 WP_074855141.1 482234..483298(+) (pilM) [Pseudomonas oleovorans strain GD04132]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKTGVKTAAV
AVAGSAVITKIIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLEAQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=730616 N5O83_RS02270 WP_074855141.1 482234..483298(+) (pilM) [Pseudomonas oleovorans strain GD04132]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTATGCCGTCGAGCCACTTCCGCCCAATGCAGTGGTCGAGAAGAACA
TTGCCGAGTTGGAGGGGGTCGGTCAGGCGCTTTCTCGCGTGTTGGCCAAGGCCAAGACCGGTGTCAAGACGGCCGCGGTT
GCTGTGGCGGGTTCGGCTGTCATCACCAAGATCATCGAAATGGAAGCGGGTCTTTCTGAGGACGACTTGGAAAACCAGCT
GAAGATCGAGGCCGATCAATACATTCCTTACCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGGCCGGCGCCGC
GTAACCCCGAACGGGTAGAGGTGCTGCTGGCTGCATGCCGTAAGGAGAACGTCGAGGTCCGCGAGGCAGCCCTTGCGCTC
GCTGGGCTTACCGCGAAAGTGGTCGATGTCGAGGCGTATGCCCTCGAACGCGCTTACGGCCTGCTGGAGGCGCAGTTGGG
TGGTGGTCACGATGAGTTGACCGTCGCGGTGGTGGATATTGGCGCGACGATGACCACGCTCAGTGTGCTGCACAACGGTC
GTACCATCTACACCCGCGAGCAGCTTTTCGGCGGCAAGCAGCTGACCGAGGAAATCCAGCGTCGCTATGGCCTGTCGGTC
GAGGAAGCCGGTCTTGCCAAGAAGCAAGGTGGTCTTCCGGATGACTACGACAGTGAGGTTTTGCAACCGTTCAAGGAAGC
GGTCGTTCAGCAGGTTTCACGTTCGCTGCAATTCTTCTTCGCCGCCGGTCAATTCAATGATGTCGACTACATCCTCCTGG
CTGGGGGTACCGCGTCCATTCCTGATCTCGATCGGCTGATCCAGCAAAAAATCGGTACGCAGACGCTAGTGGCCAATCCC
TTTGCCGATATGGCGCTCAGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S7FHW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449