Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NQS35_RS13555 Genome accession   NZ_CP104494
Coordinates   2832489..2834216 (+) Length   575 a.a.
NCBI ID   WP_016723793.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain Cw717     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2827489..2839216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQS35_RS13525 (NQS35_13485) obgE 2827543..2828643 (-) 1101 WP_011002727.1 GTPase ObgE -
  NQS35_RS13530 (NQS35_13490) rpmA 2828820..2829080 (-) 261 WP_003271630.1 50S ribosomal protein L27 -
  NQS35_RS13535 (NQS35_13495) rplU 2829118..2829429 (-) 312 WP_011002728.1 50S ribosomal protein L21 -
  NQS35_RS13540 (NQS35_13500) ispB 2829708..2830676 (+) 969 WP_011002729.1 octaprenyl diphosphate synthase -
  NQS35_RS13550 (NQS35_13510) - 2830964..2832241 (+) 1278 WP_016723792.1 HlyC/CorC family transporter -
  NQS35_RS13555 (NQS35_13515) pilF 2832489..2834216 (+) 1728 WP_016723793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NQS35_RS13560 (NQS35_13520) pilC 2834261..2835526 (+) 1266 WP_011002732.1 type II secretion system F family protein Machinery gene
  NQS35_RS13565 (NQS35_13525) - 2835529..2836410 (+) 882 WP_029240316.1 prepilin peptidase -
  NQS35_RS13570 (NQS35_13530) coaE 2836423..2837037 (+) 615 WP_011002734.1 dephospho-CoA kinase -
  NQS35_RS13575 (NQS35_13535) zapD 2837155..2837913 (+) 759 WP_016723794.1 cell division protein ZapD -
  NQS35_RS13580 (NQS35_13540) yacG 2837924..2838139 (+) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  NQS35_RS13585 (NQS35_13545) - 2838132..2838593 (-) 462 WP_023470258.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63766.62 Da        Isoelectric Point: 7.1546

>NTDB_id=730038 NQS35_RS13555 WP_016723793.1 2832489..2834216(+) (pilF) [Ralstonia pseudosolanacearum strain Cw717]
MTLGLSLAQSRRIAPTLLAQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQEATQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEVCNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=730038 NQS35_RS13555 WP_016723793.1 2832489..2834216(+) (pilF) [Ralstonia pseudosolanacearum strain Cw717]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCGCCCAGCTGGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCCGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTAA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGTTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTCCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCATTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGTATGCAACGG
CAGCGGCTACAAGGGCCGAGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.148

93.913

0.499
  pilB Acinetobacter baumannii D1279779

49.738

99.652

0.496
  pilB Acinetobacter baylyi ADP1

49.554

97.565

0.483
  pilB Legionella pneumophila strain ERS1305867

47.163

98.087

0.463
  pilB Vibrio cholerae strain A1552

44.964

96.696

0.435
  pilB Vibrio campbellii strain DS40M4

44.649

94.261

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

44.649

94.261

0.421
  pilB/pilB1 Synechocystis sp. PCC 6803

35.574

100

0.377
  pilF Thermus thermophilus HB27

38.434

97.739

0.376