Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K7563_RS16030 Genome accession   NZ_CP104289
Coordinates   3476979..3478709 (-) Length   576 a.a.
NCBI ID   WP_227837951.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain ACYCd.9D     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3471979..3483709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7563_RS16015 (K7563_16015) sucC 3472185..3473354 (-) 1170 WP_005418614.1 ADP-forming succinate--CoA ligase subunit beta -
  K7563_RS16020 (K7563_16020) - 3473762..3475375 (+) 1614 WP_227837954.1 ATP-binding protein -
  K7563_RS16025 (K7563_16025) pilR 3475436..3476809 (+) 1374 WP_072167645.1 sigma-54 dependent transcriptional regulator Regulator
  K7563_RS16030 (K7563_16030) pilB 3476979..3478709 (-) 1731 WP_227837951.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K7563_RS16035 (K7563_16035) - 3478773..3479192 (-) 420 WP_227837949.1 pilin -
  K7563_RS16040 (K7563_16040) pilA 3479288..3479698 (-) 411 WP_227837948.1 pilin Machinery gene
  K7563_RS16045 (K7563_16045) pilC 3480054..3481313 (+) 1260 WP_227837946.1 type II secretion system F family protein Machinery gene
  K7563_RS16050 (K7563_16050) - 3481322..3482185 (+) 864 WP_005418626.1 A24 family peptidase -
  K7563_RS16055 (K7563_16055) coaE 3482197..3482808 (+) 612 WP_227837944.1 dephospho-CoA kinase -
  K7563_RS16060 (K7563_16060) - 3482982..3483368 (-) 387 WP_065196692.1 hypothetical protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62186.06 Da        Isoelectric Point: 5.8722

>NTDB_id=728315 K7563_RS16030 WP_227837951.1 3476979..3478709(-) (pilB) [Stenotrophomonas maltophilia strain ACYCd.9D]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGIGNLDVSGGDEDISGGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPSNLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=728315 K7563_RS16030 WP_227837951.1 3476979..3478709(-) (pilB) [Stenotrophomonas maltophilia strain ACYCd.9D]
ATGAACGCCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGACGCGATGGCCAAGGCCGCTGCGGCCCGCCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TCGGTGCCGCACAGTTGGCGGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTTAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAATCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCGCTGGGCGGTGAC
GACGATGGCATTGGCAACCTGGATGTGTCCGGCGGTGACGAGGACATTTCCGGCGGCGGCGACTCCGGCATCGATGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGTGCCTCGGACATCCACT
TCGAGCCCTATGAAGACGACTATCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTGGCGCGTGCGCCGGTCAAG
TTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATTGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGACGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGTAAGACCGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGATCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATTGCGCCGTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGCCGGCTGTGCAGCAACTGCAAGCGCCCGTCCAACCTGCCCGAGCAT
GCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGACGCGGGTATCGAGCTGTATGAGGCGGTCGGCTGTGACGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGCTGGCTG
GCGGCAATGCGTTGCAGATTGCCGAGGCCGCACAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCC
GCTGCGGGCATTACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.095

100

0.554
  pilB Acinetobacter baylyi ADP1

54.623

100

0.554
  pilB Legionella pneumophila strain ERS1305867

51.845

98.785

0.512
  pilF Neisseria gonorrhoeae MS11

49.12

98.611

0.484
  pilB Vibrio cholerae strain A1552

48.776

99.306

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.306

0.453
  pilB Vibrio campbellii strain DS40M4

45.518

98.785

0.45