Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N2K12_RS02810 Genome accession   NZ_CP104279
Coordinates   599554..601299 (-) Length   581 a.a.
NCBI ID   WP_210930174.1    Uniprot ID   A0AA42HP60
Organism   Comamonas aquatica strain SCLZS63     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 594554..606299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N2K12_RS02780 - 595148..595609 (+) 462 WP_260674809.1 NUDIX domain-containing protein -
  N2K12_RS02785 - 595713..595922 (-) 210 WP_045267488.1 DNA gyrase inhibitor YacG -
  N2K12_RS02790 zapD 595949..596704 (-) 756 WP_042419933.1 cell division protein ZapD -
  N2K12_RS02795 coaE 596799..597407 (-) 609 WP_154163335.1 dephospho-CoA kinase -
  N2K12_RS02800 pilD 597410..598279 (-) 870 WP_260674810.1 A24 family peptidase Machinery gene
  N2K12_RS02805 pilC 598279..599502 (-) 1224 WP_045267485.1 type II secretion system F family protein Machinery gene
  N2K12_RS02810 pilB 599554..601299 (-) 1746 WP_210930174.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N2K12_RS02815 - 601458..602387 (-) 930 WP_210931650.1 polyprenyl synthetase family protein -
  N2K12_RS02820 rplU 602598..602909 (+) 312 WP_042417032.1 50S ribosomal protein L21 -
  N2K12_RS02825 rpmA 602928..603188 (+) 261 WP_042416998.1 50S ribosomal protein L27 -
  N2K12_RS02830 cgtA 603352..604425 (+) 1074 WP_042416995.1 Obg family GTPase CgtA -
  N2K12_RS02835 proB 604506..605642 (+) 1137 WP_042416992.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63313.63 Da        Isoelectric Point: 6.3015

>NTDB_id=728206 N2K12_RS02810 WP_210930174.1 599554..601299(-) (pilB) [Comamonas aquatica strain SCLZS63]
MNAASTASLIAQSSSNTTLAGLARALVSAGQLPEAVALETARKAHASKNSFVAQLVASGHMPAAELAFQIAQIFRTPLLD
LDTIDPQRLPRDLLDAKLCSMHKVLPLLKRGNRLVVATADPTDKDAAEKIKFTAQMGVDWVIVECDKLQRLVEATTKSAS
EALDAFNSDADFQFDEVVDAPAPDDKDSNASEVEDAPVVKFLHKMLIDAFNMRASDLHFEPYEHSYRVRFRIDGELREIA
SPPIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGADRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPE
EKGRLLDAIQRPYGMILVTGPTGSGKTVSLYSCLNLLNKPGVNISTAEDPSEINMPGVNQVNVNEKAGLTFATALRSFLR
QDPDVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKQPH
DVPHETLVEAGYEEHQLNGSWVPYKAVGCSACNNGYKGRVGIYQVMPITEAIQQIILRDGSALEIAAQARSEGVKSLRES
GLDKVRQGITSLEEVLAVTNL

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=728206 N2K12_RS02810 WP_210930174.1 599554..601299(-) (pilB) [Comamonas aquatica strain SCLZS63]
ATGAACGCCGCATCCACTGCATCCTTGATCGCCCAGTCCTCCTCGAACACGACACTGGCCGGGCTGGCCCGGGCCCTGGT
CAGCGCAGGCCAGCTCCCGGAAGCGGTTGCGCTGGAGACGGCCCGCAAGGCACACGCCAGCAAGAACAGCTTCGTGGCAC
AGCTGGTGGCATCCGGGCACATGCCTGCGGCGGAGCTGGCCTTTCAGATCGCACAGATCTTTCGTACACCGCTGCTGGAC
CTGGACACCATCGATCCCCAGCGCCTGCCCAGGGATTTGCTGGACGCCAAGCTGTGCAGCATGCACAAGGTGCTGCCCCT
GCTCAAGCGCGGCAACCGCCTGGTGGTGGCCACAGCAGACCCCACCGACAAGGACGCCGCGGAGAAGATCAAATTCACCG
CCCAGATGGGGGTGGACTGGGTCATCGTCGAGTGCGACAAACTGCAGCGGCTGGTCGAAGCCACCACCAAGAGTGCCTCT
GAGGCCCTGGACGCTTTCAATTCCGATGCCGACTTCCAATTCGACGAAGTGGTGGATGCGCCCGCCCCGGACGACAAGGA
CAGCAATGCATCCGAGGTCGAGGACGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGATTGACGCGTTCAACATGCGGG
CCTCGGACCTGCACTTTGAGCCCTACGAACACAGCTACCGCGTGCGCTTCCGGATCGATGGCGAACTGCGGGAGATCGCA
AGCCCGCCCATCGCCATCAAGGAAAAACTGGCCTCGCGCATCAAGGTGATCTCGCGACTGGACATCTCGGAAAAACGCGT
TCCCCAGGATGGGCGCATGAAGCTCAAGGTCGGTGCTGACCGCGTCATCGACTTCCGTGTGTCCACCTTGCCCACGCTGT
TCGGCGAGAAGATCGTGATCCGTATCCTGGACCCCAGCAGCGCCAGGATGGGCATCGATGCCCTGGGCTACGAGCCGGAG
GAAAAGGGACGTCTGCTGGATGCCATCCAGCGGCCCTACGGCATGATCCTGGTCACAGGGCCCACCGGCTCGGGCAAGAC
CGTGTCGCTATACAGCTGCCTGAACCTGCTGAACAAACCCGGGGTCAACATCTCCACGGCCGAAGATCCCTCGGAAATCA
ACATGCCCGGCGTCAACCAGGTCAATGTGAATGAAAAGGCCGGACTGACTTTCGCCACCGCCCTGCGATCCTTCCTGCGC
CAGGACCCGGATGTCATCATGGTCGGTGAAATCCGTGACCTGGAAACGGCCGACATCGCCATCAAGGCCGCGCAGACGGG
GCACTTGGTGCTATCCACCCTGCACACCAACGATGCGCCCACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCGT
TCAACATCGCCTCCAGCGTGATCCTGATCACGGCCCAGCGTCTGGCGCGCCGCTTGTGCCCACAATGCAAGCAGCCGCAT
GATGTGCCACACGAAACCCTGGTCGAAGCCGGTTACGAGGAACACCAGCTGAATGGCTCCTGGGTCCCCTACAAGGCTGT
GGGCTGCAGTGCCTGCAACAATGGTTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATCACCGAAGCAATCCAAC
AAATCATCCTGCGCGACGGCAGTGCCCTGGAGATTGCCGCCCAGGCCAGGAGCGAAGGCGTGAAATCGCTGCGCGAATCC
GGATTGGACAAAGTCCGGCAAGGCATCACTTCGCTGGAAGAGGTGCTGGCCGTCACCAATTTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-155)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.3

97.418

0.539
  pilB Acinetobacter baylyi ADP1

55.496

97.074

0.539
  pilF Neisseria gonorrhoeae MS11

51.865

96.902

0.503
  pilB Legionella pneumophila strain ERS1305867

50.436

98.623

0.497
  pilB Vibrio cholerae strain A1552

47.197

95.181

0.449
  pilB Vibrio campbellii strain DS40M4

45.633

96.558

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

46.927

92.427

0.434
  pilF Thermus thermophilus HB27

40.278

99.139

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.587

91.738

0.363