Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR30_RS09085 Genome accession   NZ_CP103875
Coordinates   1878157..1879566 (+) Length   469 a.a.
NCBI ID   WP_302500672.1    Uniprot ID   -
Organism   Gallibacterium salpingitidis strain FC22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1873157..1884566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR30_RS09055 (NYR30_09055) - 1873841..1874011 (-) 171 WP_197495490.1 antitoxin VbhA family protein -
  NYR30_RS09060 (NYR30_09060) dusA 1874145..1875125 (-) 981 WP_066114170.1 tRNA dihydrouridine(20/20a) synthase DusA -
  NYR30_RS09065 (NYR30_09065) - 1875112..1876504 (-) 1393 Protein_1763 chloride channel protein -
  NYR30_RS09070 (NYR30_09070) - 1876606..1876941 (-) 336 WP_066110356.1 hypothetical protein -
  NYR30_RS09075 (NYR30_09075) ampD 1877026..1877580 (-) 555 WP_302500668.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NYR30_RS09080 (NYR30_09080) pilA 1877687..1878157 (+) 471 WP_302500670.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR30_RS09085 (NYR30_09085) pilB 1878157..1879566 (+) 1410 WP_302500672.1 GspE/PulE family protein Machinery gene
  NYR30_RS09090 (NYR30_09090) - 1879559..1880764 (+) 1206 WP_302500675.1 type II secretion system F family protein -
  NYR30_RS09095 (NYR30_09095) - 1880971..1881384 (+) 414 WP_302500677.1 prepilin peptidase -
  NYR30_RS09100 (NYR30_09100) - 1881394..1882791 (-) 1398 WP_302500679.1 surface lipoprotein assembly modifier -
  NYR30_RS09105 (NYR30_09105) - 1882951..1883565 (-) 615 WP_302500681.1 factor H binding protein domain-containing protein -
  NYR30_RS09110 (NYR30_09110) - 1883621..1883845 (-) 225 WP_302500683.1 hypothetical protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 53054.31 Da        Isoelectric Point: 5.4019

>NTDB_id=726342 NYR30_RS09085 WP_302500672.1 1878157..1879566(+) (pilB) [Gallibacterium salpingitidis strain FC22]
MTYQVTTIDQQHTFTISEQQWEKNQAQYELLLRYLAVPLLEDEQQLWLAVESLDNLAACEAFAFLNGKPVEPVIIGQYAL
KQLLQSMREVTDLSHLHEATFQHYSTPTEQPTEEITAADPNEPLIQLFNQLLEQGLQSKASDIHLEPLHKSFQIRFRIDG
VLQKQETLMPQIGQRLNARLKLLARLDINELRLPQDGRFRYQTTFGETLDIRLSSLPTQYGEKIVLRLQHNTPVFTDFTE
LGMTAQQAAQFNHALAQPQGLILVTGPTGSGKSLTLYSGLHHLNQESRHIVTAEDPIELQLDGIIQTQVNHTIGLNFATL
LRSFLRQDPDIIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTALGAISRLQQLGISQHELENSLLLVIAQRLVRKRCQ
RCLTPSSDCACIDGYQGRTGIFQLLHYQQQQFTTDYPTLFAAAQTKLQQQITTIAELERVLGKEELQDG

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=726342 NYR30_RS09085 WP_302500672.1 1878157..1879566(+) (pilB) [Gallibacterium salpingitidis strain FC22]
ATGACCTATCAAGTGACTACTATCGATCAGCAACACACTTTTACTATTTCAGAACAGCAATGGGAAAAGAATCAGGCACA
ATATGAATTACTATTACGCTATTTAGCTGTTCCTTTGTTAGAAGATGAGCAACAATTATGGCTAGCCGTAGAATCATTAG
ATAATTTAGCCGCTTGCGAAGCCTTTGCCTTTTTAAATGGTAAACCTGTTGAACCTGTCATTATTGGTCAATATGCGTTA
AAACAGTTATTACAAAGTATGCGAGAAGTAACTGATCTTTCTCATCTACACGAAGCCACTTTTCAACATTATTCCACGCC
CACTGAACAACCAACAGAAGAGATTACTGCGGCAGATCCTAACGAACCGTTAATTCAGCTTTTTAATCAATTATTAGAAC
AAGGGTTGCAATCCAAAGCATCAGATATTCATTTAGAGCCTTTACATAAGAGTTTTCAAATTCGCTTTCGTATTGATGGT
GTATTACAAAAGCAAGAAACCTTAATGCCGCAAATTGGACAACGATTGAATGCTCGTTTGAAATTATTAGCTCGTTTAGA
TATTAATGAATTACGCTTGCCACAAGATGGTCGCTTCCGTTATCAAACCACTTTTGGTGAAACATTAGATATTCGCCTAT
CATCTTTACCAACCCAATATGGTGAAAAAATTGTATTACGTTTGCAACACAATACGCCTGTATTCACCGACTTTACTGAA
TTAGGAATGACAGCACAGCAAGCAGCACAATTCAATCACGCTTTAGCACAACCACAAGGACTCATTTTGGTCACAGGTCC
AACTGGTAGCGGCAAAAGTTTAACCTTGTACAGTGGCTTACATCACTTAAATCAAGAGAGTCGGCATATTGTTACTGCTG
AAGATCCCATTGAATTACAACTTGATGGCATTATTCAAACGCAAGTTAATCACACCATTGGGCTAAACTTCGCTACTTTA
TTGCGTAGTTTCTTACGCCAAGATCCTGACATCATTATGGTTGGCGAAATTCGTGATCAGGAAACGGCTGAAATTGCTTT
ACGTGCAGCGCAAACAGGGCACCTTGTTTTATCCACACTCCATACCAACACTGCATTAGGTGCAATTTCTCGTTTACAAC
AATTAGGTATCTCACAACATGAATTGGAAAACAGCTTACTATTAGTGATTGCACAACGCTTAGTCCGCAAACGTTGTCAG
CGATGTCTTACTCCATCATCAGATTGTGCTTGTATTGATGGCTACCAAGGACGCACAGGGATTTTTCAATTATTACATTA
CCAACAACAGCAATTTACTACTGATTACCCAACACTCTTTGCTGCCGCACAAACAAAACTCCAACAACAGATCACGACAA
TAGCAGAATTAGAGCGAGTATTAGGTAAAGAGGAACTGCAAGATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-397)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-87)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

57.918

98.294

0.569
  pilB Haemophilus influenzae Rd KW20

57.701

98.294

0.567
  pilB Glaesserella parasuis strain SC1401

53.305

100

0.533
  pilB Vibrio cholerae strain A1552

40.166

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.412
  pilB Vibrio campbellii strain DS40M4

39.167

100

0.401
  pilB Acinetobacter baylyi ADP1

37.827

100

0.401
  pilB Acinetobacter baumannii D1279779

40.868

93.39

0.382
  pilB Legionella pneumophila strain ERS1305867

35.992

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

38.427

94.883

0.365