Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M2494_RS23315 Genome accession   NZ_AP019411
Coordinates   5227613..5229307 (-) Length   564 a.a.
NCBI ID   WP_003381201.1    Uniprot ID   A0A2V0QF19
Organism   Pseudomonas syringae pv. actinidiae strain MAFF613020     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5222613..5234307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2494_RS23285 - 5223605..5223811 (+) 207 Protein_4606 transporter -
  M2494_RS23290 - 5224101..5224427 (+) 327 WP_019334445.1 hypothetical protein -
  M2494_RS23295 (KPSA1B_104748) yacG 5224693..5224902 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  M2494_RS23300 (KPSA1B_104749) coaE 5224899..5225522 (-) 624 WP_003381205.1 dephospho-CoA kinase -
  M2494_RS23305 (KPSA1B_104750) pilD 5225519..5226391 (-) 873 WP_003381203.1 A24 family peptidase Machinery gene
  M2494_RS23310 (KPSA1B_104751) pilC 5226393..5227610 (-) 1218 WP_003381202.1 type II secretion system F family protein Machinery gene
  M2494_RS23315 (KPSA1B_104752) pilB 5227613..5229307 (-) 1695 WP_003381201.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M2494_RS23320 - 5229533..5229925 (+) 393 WP_003381200.1 pilin -
  M2494_RS23325 (KPSA1B_104753) - 5229971..5231398 (+) 1428 WP_017699050.1 O-antigen ligase family protein -
  M2494_RS23330 (KPSA1B_104754) - 5231511..5232797 (+) 1287 WP_017699051.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  M2494_RS23335 (KPSA1B_104755) - 5232878..5233234 (-) 357 WP_003381197.1 helix-turn-helix domain-containing protein -
  M2494_RS23340 (KPSA1B_104756) - 5233391..5233996 (+) 606 WP_019717042.1 glutathione S-transferase N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62539.83 Da        Isoelectric Point: 6.2561

>NTDB_id=72634 M2494_RS23315 WP_003381201.1 5227613..5229307(-) (pilB) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
MSDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKVSLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLAGLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDTESGLGELADIDLGLD
IEPASDKETSLANQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=72634 M2494_RS23315 WP_003381201.1 5227613..5229307(-) (pilB) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
ATGTCTGATGCTGTCCTCACTGGTTTGGCCAAACAATTGGTCCTGGCCGAGTTACTTACTGAAACCGCTGCGCAGCAGGC
TTACCAACAGGCGCGCCGCGACAAGGTTTCACTGGTGAGCTATCTGGTCCAGAACAAACTGGTCAAAAGCCTGACGCTTG
CAGAAATGGCTTCGGACCAGTTTGGCGTGCCCTTCATGGATCTTGCCGGTCTGGACAAGGAAAGCCAGCCCAAGGGGCTG
GTCAGTGAAAAGCTGGTCCGTCAGCATCATGCGTTGCCACTTTGGCGGCGCGGTAACAAGCTGTTTATAGGCATATCGGA
CCCGACCAACCACCAGGCCGTCACAGACATACAGTTCAGCACCGGCCTGAATACCGAAGCGATTCTGGTTGAAGATGACA
AGCTGACCATTGCCATAGACCGTTTTTTTGACACTGAAAGCGGCCTTGGCGAGTTGGCAGACATCGACCTTGGCCTTGAT
ATCGAGCCTGCGAGTGACAAGGAAACGTCACTGGCAAATCAGAGTGATGCCGATGATGCCCCGGTCGTGCGCTTCGTCAA
CAAGATGCTGATGGACGCCATACGGCTCGGTTCGTCCGACCTGCACTTTGAACCCTACGAAAAGATCTTTCGCGTGCGCC
TGCGTACCGACGGCATTTTGCATGAAGTGGCCAGGCCACCGATCCATCTGGCCAACCGGATTGCTGCACGGCTTAAGGTA
ATGGCCAGTCTCGATATCTCTGAGCGGCGTAAACCGCAGGACGGCAGGGTCAAGCTGCGAGTGTCAAAAAACAAAGCGAT
CGACTTTCGCATGAACACGCTGCCAACACTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCGACCAGCGCGCAGA
TGGGCATTGATGCACTGGGCTACGAACCGGAGCAAAAGGCGCTTTATATGGAGGCCTTGAAACAGCCCCAAGGCATGATT
CTTGTCACCGGCCCAACTGGGTCAGGCAAAACCGTTTCGCTATACACCGGTCTGAACATTCTCAACACTGTAGATATCAA
CATTTCCACAGCTGAAGACCCGGTAGAGATCAACCTGGAGGGTATCAATCAGGTCAATGTCAATCCGCGACAGGGCCTTG
ACTTCTCCCAGGCATTGCGTGCCTTTCTGCGTCAAGACCCCGACGTGATCATGGTCGGCGAAATTCGGGACCTGGAAACG
GCCGAAATTGCCATCAAAGCCTCGCAAACCGGTCACATGGTGTTATCGACCCTGCACACCAACAGCGCTGCAGAAACCCT
GACTCGCCTGCACCACATGGGCGTCGCAGCGTTCAACATCGCTACGGCAATCAACCTTATCATTGCCCAGCGGCTGGCAC
GCAAGCTATGCAGTCATTGCAAGAAAGAACTCGACATACCTCGTGAAACACTCATACGAGAGGGCTTCCCAGAAGAAAAG
ATAGGTACGTTCAAAATCTACGGCCCTGTTGGCTGTGAGCATTGCAATGGCGGTTACAGGGGCAGGGTCGGGATCTACGA
AGTAGTCAAGAAAACACCTGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAGATGCGAA
AGGACGGCTTCAACGATTTGAGGACCTCCGGCCTGTCGAAGGCCATGCAAGGCATAACTAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V0QF19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

100

0.585
  pilB Acinetobacter baylyi ADP1

57.295

99.645

0.571
  pilB Legionella pneumophila strain ERS1305867

55.221

100

0.553
  pilB Vibrio cholerae strain A1552

50.624

99.468

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.181

98.227

0.493
  pilB Vibrio campbellii strain DS40M4

49.113

100

0.491
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.399

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369

Multiple sequence alignment