Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR98_RS08250 Genome accession   NZ_CP103842
Coordinates   1989627..1991363 (+) Length   578 a.a.
NCBI ID   WP_168958954.1    Uniprot ID   A0A6V7CDJ6
Organism   Xanthomonas hortorum pv. pelargonii strain 22-324     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1984627..1996363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR98_RS08230 (NYR98_08240) pilA2 1985296..1985721 (+) 426 WP_168958950.1 pilin Machinery gene
  NYR98_RS08235 (NYR98_08245) - 1985857..1987446 (+) 1590 WP_168958951.1 phosphoethanolamine transferase -
  NYR98_RS08240 (NYR98_08250) - 1987517..1988086 (+) 570 WP_233366553.1 hypothetical protein -
  NYR98_RS08245 - 1988056..1989592 (+) 1537 Protein_1616 hypothetical protein -
  NYR98_RS08250 (NYR98_08265) pilB 1989627..1991363 (+) 1737 WP_168958954.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NYR98_RS08255 (NYR98_08270) - 1991372..1991989 (-) 618 WP_168958955.1 Fic family protein -
  NYR98_RS08260 (NYR98_08275) - 1991986..1992168 (-) 183 WP_074066024.1 YhfG family protein -
  NYR98_RS08265 (NYR98_08280) pilR 1992242..1993696 (-) 1455 WP_180336569.1 sigma-54 dependent transcriptional regulator Regulator
  NYR98_RS08270 (NYR98_08285) - 1993782..1995395 (-) 1614 WP_168958956.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62770.99 Da        Isoelectric Point: 5.6078

>NTDB_id=725914 NYR98_RS08250 WP_168958954.1 1989627..1991363(+) (pilB) [Xanthomonas hortorum pv. pelargonii strain 22-324]
MNSVATINLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKKLVSASQLAAANAVEFGMPLMDVSVFDTS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNSALSSSMG
DDDEGMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAPLP
EHALLAEGFTAEQVAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=725914 NYR98_RS08250 WP_168958954.1 1989627..1991363(+) (pilB) [Xanthomonas hortorum pv. pelargonii strain 22-324]
ATGAATTCAGTAGCTACCATCAACCTAGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACGGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGTTGCCGCAGTGGTTTGCCGAGAAGAAGCTGG
TGTCGGCGTCGCAGCTGGCGGCGGCCAATGCCGTTGAATTCGGCATGCCGTTGATGGATGTGTCGGTGTTCGACACTAGC
CAGAACGCGGTCAAGCTAGTTAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTTTTCAAGCGCGGCAACCGGTT
GTTTGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGATGACATCAAATTCCACACGAACCTGGTAGTTGAGCCGA
TTCTGGTCGACGAAGACCAGATCCGTCGCACCCTGGAGCAATGGCAAGCCAGTAATTCTGCGCTTAGCTCGTCGATGGGT
GACGACGACGAGGGGATGGGGGATCTGGACGTCTCCACCGGAGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATTCGGCGGGGTGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGATTACCGGGTGCGGTTGCGGATCGACGGCTTGCTGAAGAACGTAGCCAAGGCGCCG
GTAAAGCTGAGTCAGCGCATTGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATTCTGGACGCCAGTGCGGCCAAGCTGGGGATCGAGAAGCTGGGCTATGAGGCGGATCAGCAAAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGGATGGTGCTGGTGACCGGGCCAACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTTGGCATCCTCAACGATGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GTGTGAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGATCTGGAGACGGCCGAGATCGCAATCAAGGCGGCCCAGACCGGCCACATGGT
GTTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATTGCGCGCCTGATGAACATGGGCATTGCGCCGTACAACATCA
CTTCGTCGGTGACCTTGGTTATTGCGCAGCGCCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGGCACCGCTGCCG
GAGCACGCGCTGCTTGCCGAAGGGTTCACCGCAGAGCAGGTCGCTGCCGGAATCGAACTGTACGAGGCGGTCGGCTGCGA
TGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGTAACGCGATGCAGATCGCCGAGGCGGCGCAGAGGATCGGCATCCGCGATTTGCGGCAGTCGGCGCTGGTC
AAGGCGGCGCATGGGGTGACGAGCCTGGCTGAGATCAATCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6V7CDJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilB Vibrio cholerae strain A1552

48.511

98.789

0.479
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

97.578

0.445
  pilF Thermus thermophilus HB27

40.307

90.138

0.363