Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR66_RS04825 Genome accession   NZ_CP103829
Coordinates   1053888..1055273 (-) Length   461 a.a.
NCBI ID   WP_279456503.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 2863     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048888..1060273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR66_RS04795 (NYR66_04800) lptE 1049485..1049979 (+) 495 WP_278225775.1 LPS assembly lipoprotein LptE -
  NYR66_RS04800 (NYR66_04805) holA 1050096..1051121 (+) 1026 WP_279456500.1 DNA polymerase III subunit delta -
  NYR66_RS04805 (NYR66_04810) yacG 1051198..1051386 (-) 189 WP_115589824.1 DNA gyrase inhibitor YacG -
  NYR66_RS04810 (NYR66_04815) coaE 1051368..1052006 (-) 639 WP_279456501.1 dephospho-CoA kinase -
  NYR66_RS04815 (NYR66_04820) - 1052028..1052702 (-) 675 WP_275216987.1 prepilin peptidase -
  NYR66_RS04820 (NYR66_04825) pilC 1052702..1053895 (-) 1194 WP_279456502.1 type II secretion system F family protein Machinery gene
  NYR66_RS04825 (NYR66_04830) pilB 1053888..1055273 (-) 1386 WP_279456503.1 GspE/PulE family protein Machinery gene
  NYR66_RS04830 (NYR66_04835) - 1055294..1055732 (-) 439 Protein_934 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NYR66_RS04835 (NYR66_04840) radA 1055913..1057292 (+) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR66_RS04840 (NYR66_04845) pepB 1057358..1058644 (+) 1287 WP_279456553.1 aminopeptidase PepB -
  NYR66_RS04845 (NYR66_04850) - 1058772..1059707 (+) 936 WP_279439099.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR66_RS04850 (NYR66_04855) greB 1059717..1060205 (+) 489 WP_279456504.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52600.18 Da        Isoelectric Point: 5.3555

>NTDB_id=725720 NYR66_RS04825 WP_279456503.1 1053888..1055273(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 2863]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDRESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLTKQGKFFERSTDYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725720 NYR66_RS04825 WP_279456503.1 1053888..1055273(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 2863]
ATGCAGTATTCAGTTTGTGATATGAATAGTCAGCAAGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCATATCTATAAAAGC
TTATCGTTTAGTTTAGCCAATCGTATTATCACAAGGCTTAAACTCTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAGCAATTATTACTTACAGCGTTACACCAGCCACAAGGGTTGATTCTGGTTACGGGACCAACAGGCAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCGGGAAAGTGCCGAAATTGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGATGT
GATAACTGTTATCACGGTTATAAAGGGCGAACGGGAATTTATCAGTTATTGACGAAGCAAGGTAAATTTTTTGAAAGATC
AACCGATTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.489

99.349

0.72
  pilB Haemophilus influenzae Rd KW20

57.522

98.048

0.564
  pilB Haemophilus influenzae 86-028NP

56.541

97.831

0.553
  pilB Legionella pneumophila strain ERS1305867

40.041

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.412

100

0.425
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.423
  pilB Vibrio cholerae strain A1552

38.956

100

0.421
  pilB Acinetobacter baylyi ADP1

37.773

100

0.412
  pilB Acinetobacter baumannii D1279779

38.306

100

0.412