Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR83_RS04740 Genome accession   NZ_CP103828
Coordinates   1077643..1079028 (-) Length   461 a.a.
NCBI ID   WP_279443723.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 3009     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072643..1084028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR83_RS04710 (NYR83_04710) lptE 1073249..1073743 (+) 495 WP_279443718.1 LPS assembly lipoprotein LptE -
  NYR83_RS04715 (NYR83_04715) holA 1073851..1074876 (+) 1026 WP_279443719.1 DNA polymerase III subunit delta -
  NYR83_RS04720 (NYR83_04720) yacG 1074953..1075141 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR83_RS04725 (NYR83_04725) coaE 1075123..1075761 (-) 639 WP_279443720.1 dephospho-CoA kinase -
  NYR83_RS04730 (NYR83_04730) - 1075783..1076457 (-) 675 WP_279443721.1 prepilin peptidase -
  NYR83_RS04735 (NYR83_04735) pilC 1076457..1077650 (-) 1194 WP_279443722.1 type II secretion system F family protein Machinery gene
  NYR83_RS04740 (NYR83_04740) pilB 1077643..1079028 (-) 1386 WP_279443723.1 GspE/PulE family protein Machinery gene
  NYR83_RS04745 (NYR83_04745) pilA 1079049..1079486 (-) 438 WP_279443724.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR83_RS04750 (NYR83_04750) radA 1079667..1081046 (+) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR83_RS04755 (NYR83_04755) pepB 1081112..1082398 (+) 1287 WP_279443781.1 aminopeptidase PepB -
  NYR83_RS04760 (NYR83_04760) - 1082526..1083461 (+) 936 WP_279443726.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR83_RS04765 (NYR83_04765) greB 1083471..1083959 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52385.93 Da        Isoelectric Point: 5.2257

>NTDB_id=725694 NYR83_RS04740 WP_279443723.1 1077643..1079028(-) (pilB) [Actinobacillus equuli subsp. equuli strain 3009]
MQYSACDMNSQQLFEISDALWQKNCDEKHVLLRYLAMPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSVYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGSFFEKSTAYLDYDNLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725694 NYR83_RS04740 WP_279443723.1 1077643..1079028(-) (pilB) [Actinobacillus equuli subsp. equuli strain 3009]
ATGCAGTATTCGGCTTGTGATATGAACAGCCAGCAGCTATTTGAAATTTCAGATGCATTGTGGCAAAAAAATTGTGATGA
AAAACATGTTTTATTACGTTATTTGGCTATGCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTGAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTATTTACTTAATCAACTTTCGCCGGAACAACAATCCGTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTGATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAGCTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGATGT
GATAACTGTTATCACGGTTATAAAGGGCGAACGGGAATTTACCAATTACTTGCGAAACAAGGTAGTTTTTTTGAAAAATC
TACCGCTTATTTGGATTATGACAATTTACGAGCCAGTGCTGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.052

99.349

0.716
  pilB Haemophilus influenzae Rd KW20

57.428

97.831

0.562
  pilB Haemophilus influenzae 86-028NP

56.984

97.831

0.557
  pilB Vibrio parahaemolyticus RIMD 2210633

40.241

100

0.434
  pilB Vibrio campbellii strain DS40M4

40.333

100

0.421
  pilB Legionella pneumophila strain ERS1305867

39.431

100

0.421
  pilB Vibrio cholerae strain A1552

39.834

100

0.416
  pilB Acinetobacter baylyi ADP1

38.229

100

0.412
  pilB Acinetobacter baumannii D1279779

38.889

100

0.41