Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR83_RS04745 Genome accession   NZ_CP103828
Coordinates   1079049..1079486 (-) Length   145 a.a.
NCBI ID   WP_279443724.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 3009     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1074049..1084486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR83_RS04720 (NYR83_04720) yacG 1074953..1075141 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR83_RS04725 (NYR83_04725) coaE 1075123..1075761 (-) 639 WP_279443720.1 dephospho-CoA kinase -
  NYR83_RS04730 (NYR83_04730) - 1075783..1076457 (-) 675 WP_279443721.1 prepilin peptidase -
  NYR83_RS04735 (NYR83_04735) pilC 1076457..1077650 (-) 1194 WP_279443722.1 type II secretion system F family protein Machinery gene
  NYR83_RS04740 (NYR83_04740) pilB 1077643..1079028 (-) 1386 WP_279443723.1 GspE/PulE family protein Machinery gene
  NYR83_RS04745 (NYR83_04745) pilA 1079049..1079486 (-) 438 WP_279443724.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR83_RS04750 (NYR83_04750) radA 1079667..1081046 (+) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR83_RS04755 (NYR83_04755) pepB 1081112..1082398 (+) 1287 WP_279443781.1 aminopeptidase PepB -
  NYR83_RS04760 (NYR83_04760) - 1082526..1083461 (+) 936 WP_279443726.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR83_RS04765 (NYR83_04765) greB 1083471..1083959 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15500.85 Da        Isoelectric Point: 7.5775

>NTDB_id=725695 NYR83_RS04745 WP_279443724.1 1079049..1079486(-) (pilA) [Actinobacillus equuli subsp. equuli strain 3009]
MKFVFKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGEVKNCSSGTNGIKN
GDDIKNSKYLASISVASGVITVKGKGSVDGYGYTLTPDYSNKTLTWETACLGEDTSLFPAAFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725695 NYR83_RS04745 WP_279443724.1 1079049..1079486(-) (pilA) [Actinobacillus equuli subsp. equuli strain 3009]
ATGAAATTCGTATTCAAGCCTATACAGCAGGCATTTACCCTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTAGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACGGGAGAGGTAAAAAACTGTTCTAGCGGAACGAATGGCATCAAAAAT
GGTGACGATATTAAAAACTCTAAGTATTTAGCCTCTATTTCTGTAGCTTCCGGTGTAATAACTGTGAAGGGGAAAGGGAG
TGTAGATGGGTATGGATATACGCTTACACCGGATTATTCAAATAAAACGCTAACTTGGGAAACGGCTTGTTTAGGCGAGG
ATACTAGTCTATTCCCTGCCGCGTTTTGTTCGAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

65.517

100

0.655
  pilA Haemophilus influenzae Rd KW20

56.738

97.241

0.552
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  pilA Vibrio campbellii strain DS40M4

37.589

97.241

0.366