Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR81_RS03015 Genome accession   NZ_CP103826
Coordinates   658234..659619 (+) Length   461 a.a.
NCBI ID   WP_279479637.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3113     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 653234..664619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR81_RS02990 (NYR81_02990) greB 653302..653790 (-) 489 WP_005611555.1 transcription elongation factor GreB -
  NYR81_RS02995 (NYR81_02995) - 653800..654735 (-) 936 WP_279442713.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR81_RS03000 (NYR81_03000) pepB 654863..656149 (-) 1287 WP_279479825.1 aminopeptidase PepB -
  NYR81_RS03005 (NYR81_03005) radA 656215..657595 (-) 1381 Protein_575 DNA repair protein RadA -
  NYR81_RS03010 (NYR81_03010) pilA 657776..658213 (+) 438 WP_279442711.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR81_RS03015 (NYR81_03015) pilB 658234..659619 (+) 1386 WP_279479637.1 GspE/PulE family protein Machinery gene
  NYR81_RS03020 (NYR81_03020) pilC 659612..660805 (+) 1194 WP_279442709.1 type II secretion system F family protein Machinery gene
  NYR81_RS03025 (NYR81_03025) - 660805..661479 (+) 675 WP_279442708.1 prepilin peptidase -
  NYR81_RS03030 (NYR81_03030) coaE 661501..662139 (+) 639 WP_279442707.1 dephospho-CoA kinase -
  NYR81_RS03035 (NYR81_03035) yacG 662121..662309 (+) 189 WP_115589824.1 DNA gyrase inhibitor YacG -
  NYR81_RS03040 (NYR81_03040) holA 662386..663411 (-) 1026 WP_279442706.1 DNA polymerase III subunit delta -
  NYR81_RS03045 (NYR81_03045) lptE 663519..664013 (-) 495 WP_279442705.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52485.11 Da        Isoelectric Point: 5.3508

>NTDB_id=725648 NYR81_RS03015 WP_279479637.1 658234..659619(+) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3113]
MQYSVCDMNSQQLFEISDVLWQKNCDEKHILLRYLAMPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSVYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLQIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725648 NYR81_RS03015 WP_279479637.1 658234..659619(+) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3113]
ATGCAGTATTCGGTTTGTGATATGAACAGCCAGCAGCTATTTGAAATTTCAGATGTATTGTGGCAGAAAAATTGTGATGA
AAAACATATTTTATTACGTTATTTGGCTATGCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTGAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTATTTACTTAATCAACTTTCGCCGGAACAACAATCTGTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAACTACTCAATCAAAATGACCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGACATTCACTTTGAACCGCAAAAAGAGAAGCTACAGATTAGATTACGTATTGATGGGGTATTACATATCTATAAAAGT
TTATCGTTTAGCTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTAGATATTAGCGAAACACGTTTACC
ACAAGACGGCCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTGATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTGCTATTCAATTGGATTTTAGTCAATTACTCAGAACTTTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.489

99.349

0.72
  pilB Haemophilus influenzae Rd KW20

57.428

97.831

0.562
  pilB Haemophilus influenzae 86-028NP

56.984

97.831

0.557
  pilB Vibrio parahaemolyticus RIMD 2210633

39.638

100

0.427
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.423
  pilB Legionella pneumophila strain ERS1305867

39.431

100

0.421
  pilB Vibrio cholerae strain A1552

39.627

100

0.414
  pilB Acinetobacter baumannii D1279779

38.683

100

0.408
  pilB Acinetobacter baylyi ADP1

37.903

100

0.408