Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR67_RS04630 Genome accession   NZ_CP103813
Coordinates   1052622..1054007 (-) Length   461 a.a.
NCBI ID   WP_279471326.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4130     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047622..1059007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR67_RS04600 (NYR67_04600) lptE 1048225..1048719 (+) 495 WP_278225775.1 LPS assembly lipoprotein LptE -
  NYR67_RS04605 (NYR67_04605) holA 1048827..1049852 (+) 1026 WP_279471321.1 DNA polymerase III subunit delta -
  NYR67_RS04610 (NYR67_04610) yacG 1049929..1050117 (-) 189 WP_279471322.1 DNA gyrase inhibitor YacG -
  NYR67_RS04615 (NYR67_04615) coaE 1050099..1050737 (-) 639 WP_279471323.1 dephospho-CoA kinase -
  NYR67_RS04620 (NYR67_04620) - 1050759..1051433 (-) 675 WP_279471324.1 prepilin peptidase -
  NYR67_RS04625 (NYR67_04625) - 1051433..1052629 (-) 1197 WP_279471325.1 type II secretion system F family protein -
  NYR67_RS04630 (NYR67_04630) pilB 1052622..1054007 (-) 1386 WP_279471326.1 GspE/PulE family protein Machinery gene
  NYR67_RS04635 (NYR67_04635) pilA 1054028..1054465 (-) 438 WP_279437885.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR67_RS04640 (NYR67_04640) radA 1054646..1056025 (+) 1380 WP_014991736.1 DNA repair protein RadA Machinery gene
  NYR67_RS04645 (NYR67_04645) pepB 1056091..1057377 (+) 1287 WP_279471375.1 aminopeptidase PepB -
  NYR67_RS04650 (NYR67_04650) - 1057505..1058440 (+) 936 WP_279471327.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR67_RS04655 (NYR67_04655) greB 1058450..1058938 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52526.18 Da        Isoelectric Point: 5.3508

>NTDB_id=725332 NYR67_RS04630 WP_279471326.1 1052622..1054007(-) (pilB) [Actinobacillus equuli subsp. equuli strain 4130]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFLPEQ
KQLLLAALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRTIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLATNITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725332 NYR67_RS04630 WP_279471326.1 1052622..1054007(-) (pilB) [Actinobacillus equuli subsp. equuli strain 4130]
ATGCAGTATTCGGTTTGTGATATGAATAGTCAGCAAGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAGCTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGTTTAAAACAGAATGCTT
CAGATATTCACTTTGAACCGCAAAAGGAAAAGTTGCTGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACGGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTTGCCGGAACAA
AAACAATTACTACTGGCAGCACTACATCAGCCACAAGGGCTGATTCTGGTGACAGGACCTACGGGTAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACGGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGTACTATTCAATTGGATTTTAGTCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCTAGTGCCGAACAAAAGTTAGCGACTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.926

99.349

0.725
  pilB Haemophilus influenzae Rd KW20

57.206

97.831

0.56
  pilB Haemophilus influenzae 86-028NP

56.541

97.831

0.553
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

40.333

100

0.421
  pilB Vibrio cholerae strain A1552

38.956

100

0.421
  pilB Vibrio campbellii strain DS40M4

40

100

0.421
  pilB Acinetobacter baumannii D1279779

38.508

100

0.414
  pilB Acinetobacter baylyi ADP1

37.773

100

0.412