Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   PUW74_RS04830 Genome accession   NZ_CP118080
Coordinates   907025..907678 (+) Length   217 a.a.
NCBI ID   WP_000461744.1    Uniprot ID   Q8E0F2
Organism   Streptococcus agalactiae strain VSI14     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 902025..912678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW74_RS04805 (PUW74_04805) - 902191..903021 (+) 831 WP_000735507.1 MetQ/NlpA family ABC transporter substrate-binding protein -
  PUW74_RS04810 (PUW74_04810) - 903256..904842 (+) 1587 WP_000673089.1 DEAD/DEAH box helicase -
  PUW74_RS04815 (PUW74_04815) - 905027..905293 (-) 267 WP_000598736.1 GIY-YIG nuclease family protein -
  PUW74_RS04820 (PUW74_04820) - 905286..906050 (-) 765 WP_000567425.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PUW74_RS04825 (PUW74_04825) - 906185..906925 (+) 741 WP_000500220.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  PUW74_RS04830 (PUW74_04830) comEA/celA/cilE 907025..907678 (+) 654 WP_000461744.1 helix-hairpin-helix domain-containing protein Machinery gene
  PUW74_RS04835 (PUW74_04835) comEC/celB 907662..909899 (+) 2238 WP_000939903.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PUW74_RS04840 (PUW74_04840) - 910025..910834 (+) 810 WP_000153219.1 Cof-type HAD-IIB family hydrolase -
  PUW74_RS04845 (PUW74_04845) - 910845..911789 (+) 945 WP_000200830.1 LacI family DNA-binding transcriptional regulator -

Sequence

Protein


Download         Length: 217 a.a.        Molecular weight: 23713.07 Da        Isoelectric Point: 9.5543

>NTDB_id=721218 PUW74_RS04830 WP_000461744.1 907025..907678(+) (comEA/celA/cilE) [Streptococcus agalactiae strain VSI14]
MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA
VNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID

Nucleotide


Download         Length: 654 bp        

>NTDB_id=721218 PUW74_RS04830 WP_000461744.1 907025..907678(+) (comEA/celA/cilE) [Streptococcus agalactiae strain VSI14]
ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTATTTGG
AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACAATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA
TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA
GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAAGCAA
TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA
AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATCGCTACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA
GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTGGGGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA
AGGTGGTTTTAAAACTATTGATGACCTCAAAAACGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG
TTACAATTGACTAA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(75-128)

Helix-hairpin-helix motif (HHH_3)

(153-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E0F2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

45

100

0.456
  comEA/celA/cilE Streptococcus pneumoniae Rx1

43.694

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae D39

43.694

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae R6

43.694

100

0.447
  comEA/celA/cilE Streptococcus mitis NCTC 12261

43.243

100

0.442
  comEA/celA/cilE Streptococcus mitis SK321

43.636

100

0.442
  comEA Lactococcus lactis subsp. cremoris KW2

39.013

100

0.401
  comEA Streptococcus thermophilus LMD-9

54.545

70.968

0.387