NTDB

Overview

Name	comGB	Type	Machinery gene
Locus tag	PUW62_RS00995	Genome accession	NZ_CP118046
Coordinates	189146..190168 (+)	Length	340 a.a.
NCBI ID	WP_024053129.1	Uniprot ID	-
Organism	Streptococcus anginosus strain VSI52
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 184146..195168

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW62_RS00970 (PUW62_00970)	-	185290..185820 (+)	531	WP_024053133.1	aromatic acid exporter family protein	-
PUW62_RS00975 (PUW62_00975)	-	185897..186256 (+)	360	WP_003070793.1	MerR family transcriptional regulator	-
PUW62_RS00980 (PUW62_00980)	glnA	186294..187640 (+)	1347	WP_024053132.1	type I glutamate--ammonia ligase	-
PUW62_RS00985 (PUW62_00985)	-	187828..188193 (+)	366	WP_024053131.1	DUF1033 family protein	-
PUW62_RS00990 (PUW62_00990)	comGA	188263..189204 (+)	942	WP_024053130.1	competence type IV pilus ATPase ComGA	Machinery gene
PUW62_RS00995 (PUW62_00995)	comGB	189146..190168 (+)	1023	WP_024053129.1	competence type IV pilus assembly protein ComGB	Machinery gene
PUW62_RS01000 (PUW62_01000)	comGC	190165..190482 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
PUW62_RS01005 (PUW62_01005)	comGD	190472..190870 (+)	399	WP_022525973.1	competence type IV pilus minor pilin ComGD	Machinery gene
PUW62_RS01010 (PUW62_01010)	comGE/cglE	190842..191135 (+)	294	WP_024053127.1	competence type IV pilus minor pilin ComGE	Machinery gene
PUW62_RS01015 (PUW62_01015)	comGF/cglF	191119..191556 (+)	438	WP_024053126.1	competence type IV pilus minor pilin ComGF	Machinery gene
PUW62_RS01020 (PUW62_01020)	comGG/cglG	191537..191863 (+)	327	WP_022525970.1	competence type IV pilus minor pilin ComGG	Machinery gene
PUW62_RS01025 (PUW62_01025)	comGH	191935..192888 (+)	954	WP_024053125.1	class I SAM-dependent methyltransferase	Machinery gene
PUW62_RS01030 (PUW62_01030)	-	192935..194134 (+)	1200	WP_024053124.1	acetate kinase	-

Sequence

Protein

Download Length: 340 a.a. Molecular weight: 39117.25 Da Isoelectric Point: 9.2646

                                >NTDB_id=720630 PUW62_RS00995 WP_024053129.1 189146..190168(+) (comGB) [Streptococcus anginosus strain VSI52]

MQQDISILSRQKRKKLSTVRQKKVVELLNNLFSSGFHLAEIIDFLKRSALLEEVYVDRMKEELSTGKPFSDIMASLGFSD

NVVTQLSLAELHGNLSLSLSKIEEYLENISKIKKKLVEVATYPFILFVFLIFIMLGLRNYLLPQLDCQNIATQIISNLPQ

LFLGSLGVVFVLLAIGFYWFQKSSKIAVFRLLARLPFLGAFIQTYLTAYYAREWGNMIGQGLELSQIFQMMQGQRSAMFQ

EIGKDLEVALQNGQEFSQRVKTYPFFKKELGLMIEYGEVKSKLGCELEVYAQKTWETFFKRVHQAMNVIQPLVFVFVALM

IVLLYAAMLLPIYQNMEVQL

Nucleotide

Download Length: 1023 bp

                                >NTDB_id=720630 PUW62_RS00995 WP_024053129.1 189146..190168(+) (comGB) [Streptococcus anginosus strain VSI52]

ATGCAGCAGGACATATCAATCTTGAGCAGGCAGAAGCGGAAAAAATTATCCACAGTTAGGCAGAAAAAGGTCGTAGAGTT

GCTGAATAATCTTTTTTCTAGTGGCTTTCATTTGGCAGAAATTATCGACTTTTTGAAGCGAAGTGCCTTGTTAGAAGAAG

TCTATGTGGACAGGATGAAAGAGGAGTTGTCAACAGGAAAGCCTTTTTCGGACATTATGGCAAGCTTAGGTTTTTCAGAT

AACGTGGTGACACAGCTTTCGCTGGCAGAATTACACGGAAATCTCTCACTCAGCCTGAGTAAGATTGAGGAATACTTGGA

AAATATCTCAAAAATTAAGAAGAAGTTGGTTGAAGTGGCGACTTATCCCTTTATTTTGTTTGTTTTTTTGATATTCATTA

TGCTGGGATTACGTAATTATTTACTCCCGCAACTCGATTGTCAAAATATAGCAACTCAGATTATCAGTAATTTGCCGCAA

CTTTTTCTGGGTTCATTAGGGGTAGTCTTTGTTTTGTTAGCGATAGGTTTTTATTGGTTTCAAAAAAGCTCAAAAATAGC

AGTATTTCGTTTGTTAGCTCGCCTTCCTTTTTTGGGCGCTTTTATTCAAACCTATTTAACAGCTTATTATGCGAGAGAAT

GGGGGAATATGATTGGTCAAGGTTTAGAACTCAGTCAGATTTTTCAGATGATGCAAGGACAACGCTCTGCTATGTTTCAA

GAAATTGGAAAAGATTTAGAAGTTGCGCTGCAAAATGGTCAGGAATTTTCGCAAAGAGTTAAAACATATCCATTTTTTAA

AAAGGAATTGGGCTTGATGATCGAATACGGTGAAGTAAAATCCAAATTGGGGTGTGAATTAGAAGTTTATGCCCAAAAAA

CATGGGAGACATTTTTTAAACGTGTTCATCAAGCGATGAATGTCATTCAACCACTAGTGTTTGTCTTTGTGGCTTTAATG

ATTGTATTGTTGTATGCAGCCATGTTGCTGCCAATTTATCAAAATATGGAGGTTCAATTATGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(26-143)

Type II secretion system (T2SS), protein F (T2SSF)

(211-331)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGB	Streptococcus gordonii str. Challis substr. CH1	70.294	100	0.703
comGB/cglB	Streptococcus mitis SK321	68.657	98.529	0.676
comGB/cglB	Streptococcus mitis NCTC 12261	68.657	98.529	0.676
comGB/cglB	Streptococcus pneumoniae Rx1	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae D39	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae R6	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae TIGR4	67.761	98.529	0.668
comGB	Streptococcus mutans UA140	59.649	100	0.6
comGB	Streptococcus mutans UA159	59.649	100	0.6
comGB	Lactococcus lactis subsp. cremoris KW2	51.039	99.118	0.506

Detailed information NTG0720630