Detailed information NTG0720634

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: PUW62_RS01015
• Genome accession: NZ_CP118046
• Coordinates: 191119..191556 (+)
• Length: 145 a.a.
• NCBI ID: WP_024053126.1
• Uniprot ID: A0A413KLZ8
• Organism: Streptococcus anginosus strain VSI52
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 186119..196556

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW62_RS00980 (PUW62_00980)	glnA	186294..187640 (+)	1347	WP_024053132.1	type I glutamate--ammonia ligase	-
PUW62_RS00985 (PUW62_00985)	-	187828..188193 (+)	366	WP_024053131.1	DUF1033 family protein	-
PUW62_RS00990 (PUW62_00990)	comGA	188263..189204 (+)	942	WP_024053130.1	competence type IV pilus ATPase ComGA	Machinery gene
PUW62_RS00995 (PUW62_00995)	comGB	189146..190168 (+)	1023	WP_024053129.1	competence type IV pilus assembly protein ComGB	Machinery gene
PUW62_RS01000 (PUW62_01000)	comGC	190165..190482 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
PUW62_RS01005 (PUW62_01005)	comGD	190472..190870 (+)	399	WP_022525973.1	competence type IV pilus minor pilin ComGD	Machinery gene
PUW62_RS01010 (PUW62_01010)	comGE/cglE	190842..191135 (+)	294	WP_024053127.1	competence type IV pilus minor pilin ComGE	Machinery gene
PUW62_RS01015 (PUW62_01015)	comGF/cglF	191119..191556 (+)	438	WP_024053126.1	competence type IV pilus minor pilin ComGF	Machinery gene
PUW62_RS01020 (PUW62_01020)	comGG/cglG	191537..191863 (+)	327	WP_022525970.1	competence type IV pilus minor pilin ComGG	Machinery gene
PUW62_RS01025 (PUW62_01025)	comGH	191935..192888 (+)	954	WP_024053125.1	class I SAM-dependent methyltransferase	Machinery gene
PUW62_RS01030 (PUW62_01030)	-	192935..194134 (+)	1200	WP_024053124.1	acetate kinase	-
PUW62_RS01035 (PUW62_01035)	glmS	194529..196343 (+)	1815	WP_024053123.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16883.44 Da
• Isoelectric Point: 10.0408

>NTDB_id=720634 PUW62_RS01015 WP_024053126.1 191119..191556(+) (comGF/cglF) [Streptococcus anginosus strain VSI52]
MFKANKVKAFTLLEALIVLFVLSGSVLLFQSMTRLLAFEVQARQHSEQREWLLFADQLEAELARSSFEKVENNRLYMKQD
GRAIAFGKSSGQDFRKTNANGKGYQPMVYDVKKAEISQKNELIYVRLTFRRGLEREFVYRVETES

Nucleotide

• Length: 438 bp

>NTDB_id=720634 PUW62_RS01015 WP_024053126.1 191119..191556(+) (comGF/cglF) [Streptococcus anginosus strain VSI52]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACCCTTTTAGAGGCTCTGATTGTCTTATTTGTACTCAGCGGAAGTGTGCT
GCTTTTTCAGTCAATGACGCGTCTTCTGGCTTTTGAAGTTCAAGCCCGGCAACATAGTGAACAACGAGAATGGCTTTTGT
TTGCTGATCAACTAGAAGCAGAGTTGGCTCGTTCTTCATTTGAAAAGGTGGAGAATAATCGTTTATATATGAAACAGGAC
GGCAGGGCAATTGCATTTGGAAAGTCAAGTGGACAGGATTTCAGAAAAACCAATGCGAATGGGAAAGGTTACCAGCCGAT
GGTTTATGATGTAAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTTATGTTAGACTGACGTTTAGGCGAGGATTGG
AAAGGGAGTTTGTCTATCGTGTGGAAACAGAAAGTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A413KLZ8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	64.085	97.931	0.628
comGF/cglF	Streptococcus pneumoniae TIGR4	64.085	97.931	0.628
comGF/cglF	Streptococcus pneumoniae Rx1	64.085	97.931	0.628
comGF/cglF	Streptococcus pneumoniae D39	64.085	97.931	0.628
comGF/cglF	Streptococcus mitis NCTC 12261	64.286	96.552	0.621
comGF/cglF	Streptococcus mitis SK321	64.286	96.552	0.621
comGF	Streptococcus mutans UA159	42.963	93.103	0.4
comGF	Streptococcus mutans UA140	42.963	93.103	0.4
comGF	Lactococcus lactis subsp. cremoris KW2	40	93.103	0.372