Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   PUW62_RS01010 Genome accession   NZ_CP118046
Coordinates   190842..191135 (+) Length   97 a.a.
NCBI ID   WP_024053127.1    Uniprot ID   A0A412PLL4
Organism   Streptococcus anginosus strain VSI52     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 185842..196135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW62_RS00975 (PUW62_00975) - 185897..186256 (+) 360 WP_003070793.1 MerR family transcriptional regulator -
  PUW62_RS00980 (PUW62_00980) glnA 186294..187640 (+) 1347 WP_024053132.1 type I glutamate--ammonia ligase -
  PUW62_RS00985 (PUW62_00985) - 187828..188193 (+) 366 WP_024053131.1 DUF1033 family protein -
  PUW62_RS00990 (PUW62_00990) comGA 188263..189204 (+) 942 WP_024053130.1 competence type IV pilus ATPase ComGA Machinery gene
  PUW62_RS00995 (PUW62_00995) comGB 189146..190168 (+) 1023 WP_024053129.1 competence type IV pilus assembly protein ComGB Machinery gene
  PUW62_RS01000 (PUW62_01000) comGC 190165..190482 (+) 318 WP_003028473.1 competence type IV pilus major pilin ComGC Machinery gene
  PUW62_RS01005 (PUW62_01005) comGD 190472..190870 (+) 399 WP_022525973.1 competence type IV pilus minor pilin ComGD Machinery gene
  PUW62_RS01010 (PUW62_01010) comGE/cglE 190842..191135 (+) 294 WP_024053127.1 competence type IV pilus minor pilin ComGE Machinery gene
  PUW62_RS01015 (PUW62_01015) comGF/cglF 191119..191556 (+) 438 WP_024053126.1 competence type IV pilus minor pilin ComGF Machinery gene
  PUW62_RS01020 (PUW62_01020) comGG/cglG 191537..191863 (+) 327 WP_022525970.1 competence type IV pilus minor pilin ComGG Machinery gene
  PUW62_RS01025 (PUW62_01025) comGH 191935..192888 (+) 954 WP_024053125.1 class I SAM-dependent methyltransferase Machinery gene
  PUW62_RS01030 (PUW62_01030) - 192935..194134 (+) 1200 WP_024053124.1 acetate kinase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10836.84 Da        Isoelectric Point: 10.6170

>NTDB_id=720633 PUW62_RS01010 WP_024053127.1 190842..191135(+) (comGE/cglE) [Streptococcus anginosus strain VSI52]
MGNLRKQHVKGTILLEALLALAVFVTIVTLLLGQIHQSRKAENDLLREEEVLRVAKMALQTKQSHLTMNGIKVRVETSQQ
GIQIYHGKELILGVQGK

Nucleotide


Download         Length: 294 bp        

>NTDB_id=720633 PUW62_RS01010 WP_024053127.1 190842..191135(+) (comGE/cglE) [Streptococcus anginosus strain VSI52]
ATGGGAAATTTAAGAAAACAACACGTTAAAGGAACGATTTTATTAGAGGCGCTGCTGGCTTTGGCTGTTTTTGTGACAAT
TGTGACCCTGTTGTTGGGACAAATTCACCAGTCTCGAAAAGCAGAAAATGACTTGCTTAGAGAAGAAGAAGTTTTAAGGG
TAGCTAAAATGGCTTTGCAGACAAAGCAAAGCCATTTGACAATGAACGGTATTAAAGTGCGTGTTGAAACAAGTCAACAA
GGTATTCAAATTTATCATGGGAAGGAGCTGATTCTCGGTGTTCAAGGCAAATAA

Domains

Predicted by InterProScan.

Type II secretory pathway pseudopilin (ComGE)

(13-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A412PLL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus pneumoniae Rx1

50.526

97.938

0.495
  comGE/cglE Streptococcus pneumoniae D39

50.526

97.938

0.495
  comGE/cglE Streptococcus pneumoniae R6

50.526

97.938

0.495
  comGE/cglE Streptococcus pneumoniae TIGR4

50.526

97.938

0.495
  comGE/cglE Streptococcus mitis NCTC 12261

49.474

97.938

0.485
  comGE/cglE Streptococcus mitis SK321

49.474

97.938

0.485
  comGE Streptococcus mutans UA140

45.455

100

0.464
  comGE Streptococcus mutans UA159

45.455

100

0.464