Detailed information NTG0720330

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: PUW70_RS01170
• Genome accession: NZ_CP118029
• Coordinates: 209248..209685 (+)
• Length: 145 a.a.
• NCBI ID: WP_024053126.1
• Uniprot ID: A0A413KLZ8
• Organism: Streptococcus anginosus strain VSI68
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 204248..214685

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW70_RS01135 (PUW70_01135)	glnA	204423..205769 (+)	1347	WP_024053132.1	type I glutamate--ammonia ligase	-
PUW70_RS01140 (PUW70_01140)	-	205957..206322 (+)	366	WP_024053131.1	DUF1033 family protein	-
PUW70_RS01145 (PUW70_01145)	comGA	206392..207333 (+)	942	WP_024053130.1	competence type IV pilus ATPase ComGA	Machinery gene
PUW70_RS01150 (PUW70_01150)	comGB	207275..208297 (+)	1023	WP_171021869.1	competence type IV pilus assembly protein ComGB	Machinery gene
PUW70_RS01155 (PUW70_01155)	comGC	208294..208611 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
PUW70_RS01160 (PUW70_01160)	comGD	208601..208999 (+)	399	WP_022525973.1	competence type IV pilus minor pilin ComGD	Machinery gene
PUW70_RS01165 (PUW70_01165)	comGE/cglE	208971..209264 (+)	294	WP_024053127.1	competence type IV pilus minor pilin ComGE	Machinery gene
PUW70_RS01170 (PUW70_01170)	comGF/cglF	209248..209685 (+)	438	WP_024053126.1	competence type IV pilus minor pilin ComGF	Machinery gene
PUW70_RS01175 (PUW70_01175)	comGG/cglG	209666..209992 (+)	327	WP_257360478.1	competence type IV pilus minor pilin ComGG	Machinery gene
PUW70_RS01180 (PUW70_01180)	comGH	210064..211017 (+)	954	WP_024053125.1	class I SAM-dependent methyltransferase	Machinery gene
PUW70_RS01185 (PUW70_01185)	-	211064..212263 (+)	1200	WP_024053124.1	acetate kinase	-
PUW70_RS01190 (PUW70_01190)	glmS	212658..214472 (+)	1815	WP_024053123.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16883.44 Da
• Isoelectric Point: 10.0408

>NTDB_id=720330 PUW70_RS01170 WP_024053126.1 209248..209685(+) (comGF/cglF) [Streptococcus anginosus strain VSI68]
MFKANKVKAFTLLEALIVLFVLSGSVLLFQSMTRLLAFEVQARQHSEQREWLLFADQLEAELARSSFEKVENNRLYMKQD
GRAIAFGKSSGQDFRKTNANGKGYQPMVYDVKKAEISQKNELIYVRLTFRRGLEREFVYRVETES

Nucleotide

• Length: 438 bp

>NTDB_id=720330 PUW70_RS01170 WP_024053126.1 209248..209685(+) (comGF/cglF) [Streptococcus anginosus strain VSI68]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACCCTTTTAGAGGCTCTGATTGTCTTATTTGTACTCAGCGGAAGTGTGCT
GCTTTTTCAGTCAATGACGCGTCTTCTGGCTTTTGAAGTTCAAGCCCGGCAACATAGTGAACAACGAGAATGGCTTTTGT
TTGCTGATCAACTAGAAGCAGAGTTGGCTCGTTCTTCATTTGAAAAGGTGGAGAATAATCGTTTATATATGAAACAGGAC
GGCAGGGCAATTGCATTTGGAAAGTCAAGTGGACAGGATTTCAGAAAAACCAATGCGAATGGGAAAGGTTACCAGCCGAT
GGTTTATGATGTAAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTTATGTTAGACTGACGTTTAGGCGAGGATTGG
AAAGGGAGTTTGTCTATCGTGTGGAAACAGAAAGTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A413KLZ8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	64.085	97.931	0.628
comGF/cglF	Streptococcus pneumoniae TIGR4	64.085	97.931	0.628
comGF/cglF	Streptococcus pneumoniae Rx1	64.085	97.931	0.628
comGF/cglF	Streptococcus pneumoniae D39	64.085	97.931	0.628
comGF/cglF	Streptococcus mitis NCTC 12261	64.286	96.552	0.621
comGF/cglF	Streptococcus mitis SK321	64.286	96.552	0.621
comGF	Streptococcus mutans UA159	42.963	93.103	0.4
comGF	Streptococcus mutans UA140	42.963	93.103	0.4
comGF	Lactococcus lactis subsp. cremoris KW2	40	93.103	0.372