NTDB

Overview

Name	comGB	Type	Machinery gene
Locus tag	PUW70_RS01150	Genome accession	NZ_CP118029
Coordinates	207275..208297 (+)	Length	340 a.a.
NCBI ID	WP_171021869.1	Uniprot ID	A0ABS3GFA7
Organism	Streptococcus anginosus strain VSI68
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 202275..213297

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW70_RS01125 (PUW70_01125)	-	203419..203949 (+)	531	WP_070241503.1	aromatic acid exporter family protein	-
PUW70_RS01130 (PUW70_01130)	-	204026..204385 (+)	360	WP_003070793.1	MerR family transcriptional regulator	-
PUW70_RS01135 (PUW70_01135)	glnA	204423..205769 (+)	1347	WP_024053132.1	type I glutamate--ammonia ligase	-
PUW70_RS01140 (PUW70_01140)	-	205957..206322 (+)	366	WP_024053131.1	DUF1033 family protein	-
PUW70_RS01145 (PUW70_01145)	comGA	206392..207333 (+)	942	WP_024053130.1	competence type IV pilus ATPase ComGA	Machinery gene
PUW70_RS01150 (PUW70_01150)	comGB	207275..208297 (+)	1023	WP_171021869.1	competence type IV pilus assembly protein ComGB	Machinery gene
PUW70_RS01155 (PUW70_01155)	comGC	208294..208611 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
PUW70_RS01160 (PUW70_01160)	comGD	208601..208999 (+)	399	WP_022525973.1	competence type IV pilus minor pilin ComGD	Machinery gene
PUW70_RS01165 (PUW70_01165)	comGE/cglE	208971..209264 (+)	294	WP_024053127.1	competence type IV pilus minor pilin ComGE	Machinery gene
PUW70_RS01170 (PUW70_01170)	comGF/cglF	209248..209685 (+)	438	WP_024053126.1	competence type IV pilus minor pilin ComGF	Machinery gene
PUW70_RS01175 (PUW70_01175)	comGG/cglG	209666..209992 (+)	327	WP_257360478.1	competence type IV pilus minor pilin ComGG	Machinery gene
PUW70_RS01180 (PUW70_01180)	comGH	210064..211017 (+)	954	WP_024053125.1	class I SAM-dependent methyltransferase	Machinery gene
PUW70_RS01185 (PUW70_01185)	-	211064..212263 (+)	1200	WP_024053124.1	acetate kinase	-

Sequence

Protein

Download Length: 340 a.a. Molecular weight: 39170.30 Da Isoelectric Point: 9.5495

                                >NTDB_id=720326 PUW70_RS01150 WP_171021869.1 207275..208297(+) (comGB) [Streptococcus anginosus strain VSI68]

MQQDISILSRQKRKKLSTVRQKKVVELLNNLFSSGFHLAEIIDFLKRSALLEEVYVDRMKEELSTGKPFSDIMASLGFSD

NVVTQLSLAELHGNLSLSLSKIEEYLENISKIKKKLVEVATYPFILFVFLIFIMLGLRNYLLPQLDRQNIATQIISNLPQ

LFLGSLGVVFVLLAIGFYWFQKSSKIAVFRLLARLPFLGAFIQTYLTAYYAREWGNMIGQGLELSQIFQMMQGQRSAMFQ

EIGKDLEVALQNGQEFSQRVKTYPFFKKELGLMIEYGEVKSKLGCELEVYAQKTWETFFKRVHQAMNVIQPLVFVFVALM

IVLLYAAMLLPIYQNMEVQL

Nucleotide

Download Length: 1023 bp

                                >NTDB_id=720326 PUW70_RS01150 WP_171021869.1 207275..208297(+) (comGB) [Streptococcus anginosus strain VSI68]

ATGCAGCAGGACATATCAATCTTGAGCAGGCAGAAGCGGAAAAAATTATCCACAGTTAGGCAGAAAAAGGTCGTAGAGTT

GCTGAATAATCTTTTTTCTAGTGGCTTTCATTTGGCAGAAATTATCGACTTTTTGAAGCGAAGTGCCTTGTTAGAAGAAG

TCTATGTGGACAGGATGAAAGAGGAGTTGTCAACAGGAAAGCCTTTTTCGGACATTATGGCAAGCTTAGGTTTTTCAGAT

AACGTGGTGACACAGCTTTCGCTGGCAGAATTACACGGAAATCTCTCACTCAGCCTGAGTAAGATTGAGGAATACTTGGA

AAATATCTCAAAAATTAAGAAGAAGTTGGTTGAAGTGGCGACTTATCCCTTTATTTTGTTTGTTTTTTTGATATTCATTA

TGCTGGGATTACGTAATTATTTACTCCCGCAACTCGATCGTCAAAATATAGCAACTCAGATTATCAGTAATTTGCCGCAA

CTTTTTCTGGGTTCATTAGGGGTAGTCTTTGTTTTGTTAGCGATAGGTTTTTATTGGTTTCAAAAAAGCTCAAAAATAGC

AGTATTTCGTTTGTTAGCTCGCCTTCCTTTTTTGGGCGCTTTTATTCAAACCTATTTAACAGCTTATTATGCGAGAGAAT

GGGGGAATATGATTGGTCAAGGTTTAGAACTCAGTCAGATTTTTCAGATGATGCAAGGACAACGCTCTGCTATGTTTCAA

GAAATTGGAAAAGATTTAGAAGTTGCGCTGCAAAATGGTCAGGAATTTTCGCAAAGAGTTAAAACATATCCATTTTTTAA

AAAGGAATTGGGCTTGATGATCGAATACGGTGAAGTAAAATCCAAATTGGGGTGTGAATTAGAAGTTTATGCCCAAAAAA

CATGGGAGACATTTTTTAAACGTGTTCATCAAGCGATGAATGTCATTCAACCACTAGTGTTTGTCTTTGTGGCTTTAATG

ATTGTATTGTTGTATGCAGCCATGTTGCTGCCAATTTATCAAAATATGGAGGTTCAATTATGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(26-143)

Type II secretion system (T2SS), protein F (T2SSF)

(211-331)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGB	Streptococcus gordonii str. Challis substr. CH1	70.294	100	0.703
comGB/cglB	Streptococcus mitis SK321	68.657	98.529	0.676
comGB/cglB	Streptococcus mitis NCTC 12261	68.657	98.529	0.676
comGB/cglB	Streptococcus pneumoniae Rx1	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae D39	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae R6	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae TIGR4	67.761	98.529	0.668
comGB	Streptococcus mutans UA159	59.942	100	0.603
comGB	Streptococcus mutans UA140	59.649	100	0.6
comGB	Lactococcus lactis subsp. cremoris KW2	51.039	99.118	0.506

Detailed information NTG0720326