Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MKL45_RS01625 Genome accession   NZ_CP102934
Coordinates   342473..344185 (-) Length   570 a.a.
NCBI ID   WP_001274984.1    Uniprot ID   -
Organism   Acinetobacter sp. AOR07_HL     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337473..349185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKL45_RS01595 (MKL45_01595) - 337635..337961 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  MKL45_RS01600 (MKL45_01600) rlmB 338063..338812 (+) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MKL45_RS01605 (MKL45_01605) - 338848..339762 (+) 915 WP_000075015.1 DMT family transporter -
  MKL45_RS01610 (MKL45_01610) coaE 339759..340355 (-) 597 WP_030423659.1 dephospho-CoA kinase -
  MKL45_RS01615 (MKL45_01615) pilD 340357..341217 (-) 861 WP_001152273.1 A24 family peptidase Machinery gene
  MKL45_RS01620 (MKL45_01620) pilC 341217..342443 (-) 1227 WP_000279216.1 type II secretion system F family protein Machinery gene
  MKL45_RS01625 (MKL45_01625) pilB 342473..344185 (-) 1713 WP_001274984.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MKL45_RS01630 (MKL45_01630) tpiA 344476..345270 (+) 795 WP_000016939.1 triose-phosphate isomerase -
  MKL45_RS01635 (MKL45_01635) secG 345283..345612 (+) 330 WP_005129832.1 preprotein translocase subunit SecG -
  MKL45_RS01655 (MKL45_01655) rimP 346254..346778 (+) 525 WP_000777730.1 ribosome maturation factor RimP -
  MKL45_RS01660 (MKL45_01660) nusA 346816..348300 (+) 1485 WP_000532248.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63355.71 Da        Isoelectric Point: 5.2371

>NTDB_id=719941 MKL45_RS01625 WP_001274984.1 342473..344185(-) (pilB) [Acinetobacter sp. AOR07_HL]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAQDDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=719941 MKL45_RS01625 WP_001274984.1 342473..344185(-) (pilB) [Acinetobacter sp. AOR07_HL]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCATTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACTTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
CGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGCCCTACCGCTCAAGACGATGAAGAAGAAGCTCCACAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCTATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCCCCTCTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGTGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AACCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCATTAGGATATGAAGAAGATCAAAAAGCTTTGTTCATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGGATTAATCAAGTTAACGTAAATCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCAATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGTAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.474

100

0.995
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.894

92.632

0.481
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB Vibrio cholerae strain A1552

50.484

90.702

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365