Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NVV93_RS03730 Genome accession   NZ_CP102830
Coordinates   840546..842249 (-) Length   567 a.a.
NCBI ID   WP_258253112.1    Uniprot ID   -
Organism   Pseudomonas sp. LS44     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 835546..847249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NVV93_RS03695 (NVV93_03695) - 835628..835801 (-) 174 WP_258253106.1 DUF3094 domain-containing protein -
  NVV93_RS03700 (NVV93_03700) - 835887..836513 (-) 627 WP_258253107.1 DUF1780 domain-containing protein -
  NVV93_RS03705 (NVV93_03705) - 836731..837421 (+) 691 Protein_735 energy-coupling factor ABC transporter permease -
  NVV93_RS03710 (NVV93_03710) yacG 837430..837630 (-) 201 WP_258253108.1 DNA gyrase inhibitor YacG -
  NVV93_RS03715 (NVV93_03715) coaE 837627..838238 (-) 612 WP_258253109.1 dephospho-CoA kinase -
  NVV93_RS03720 (NVV93_03720) pilD 838235..839107 (-) 873 WP_258253110.1 A24 family peptidase Machinery gene
  NVV93_RS03725 (NVV93_03725) pilC 839110..840327 (-) 1218 WP_258253111.1 type II secretion system F family protein Machinery gene
  NVV93_RS03730 (NVV93_03730) pilB 840546..842249 (-) 1704 WP_258253112.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NVV93_RS03740 (NVV93_03740) - 842470..842889 (+) 420 WP_309137384.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NVV93_RS03745 (NVV93_03745) - 843032..844714 (+) 1683 WP_258253113.1 PglL family O-oligosaccharyltransferase -
  NVV93_RS03750 (NVV93_03750) nadC 844899..845747 (-) 849 WP_258253114.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62485.64 Da        Isoelectric Point: 5.7469

>NTDB_id=719142 NVV93_RS03730 WP_258253112.1 840546..842249(-) (pilB) [Pseudomonas sp. LS44]
MNDSVALSGLARQMVLAELLDEKTAQQAQQQAQRNKLPLVTYLVQNKLVKSRALAELAAEQFGVAFVDLASIDKEGQPKE
LVSEKLIRQHRALPLWRRGNKLFVALSDPSNHQAVTDIQFSTGLTTEAILVEDDKLGEAIDKFFDAANSGLEDLADVDLD
GLDIQAVDEDKQDAATGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMANLDISERRKPQDGRIKMKISKSKSIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEGQKELYMTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTADVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPHCKKEVQIPHETLLHEGFP
EDKIGTFKIYAPVGCENCKGGYKGRLGIYEVVKNTPALQRIIMEDGNSIDIGVQMRKDGFNDLRTSGLEKAMQGITSLAE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=719142 NVV93_RS03730 WP_258253112.1 840546..842249(-) (pilB) [Pseudomonas sp. LS44]
ATGAACGACAGCGTCGCCCTTTCCGGCCTGGCCAGGCAGATGGTGCTGGCCGAACTGCTAGACGAAAAAACCGCCCAGCA
GGCCCAGCAGCAGGCCCAGCGCAACAAGCTGCCGCTGGTCACCTACCTGGTGCAGAACAAGCTGGTGAAAAGCCGCGCCC
TGGCCGAACTGGCGGCCGAGCAGTTTGGCGTGGCCTTCGTCGACCTCGCCAGCATCGACAAGGAAGGCCAGCCCAAGGAG
CTGGTCAGCGAGAAACTGATCCGCCAGCACCGCGCCCTGCCGCTGTGGCGGCGCGGCAACAAGCTATTCGTCGCGCTCTC
CGACCCCAGCAATCACCAGGCGGTCACCGACATCCAGTTCAGCACCGGGCTGACCACCGAGGCCATCCTGGTCGAGGACG
ACAAGCTCGGCGAGGCCATCGACAAGTTCTTCGACGCCGCCAACAGCGGCTTGGAAGACCTTGCCGACGTCGATCTCGAT
GGGCTGGATATCCAGGCCGTCGATGAAGACAAGCAGGACGCCGCCACCGGCCAGGACGCCGACGACGCGCCAGTGGTGCG
CTTCGTCAACAAGATGCTCCTGGATGCGATCAAAGGCGGCTCGTCCGACCTGCACTTCGAGCCCTATGAAAAGGTCTACC
GCGTGCGCCTGCGCACCGACGGCATCCTCCACGAGGTCGCCCGCCCGCCGATCCAGCTGGCTCCGCGTATCTCCGCGCGC
CTGAAGGTGATGGCCAACTTGGACATTTCCGAGCGGCGCAAGCCGCAGGACGGCCGGATAAAGATGAAGATCTCCAAGTC
CAAGTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAGATCGTCATGCGGATTCTCGACCCTACCA
GCGCGCAGATGGGTATCGATGCCCTGGGCTATGAGGAAGGTCAGAAAGAGCTGTACATGACCGCCCTCAAGCAACCCCAG
GGCATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTATCGCTGTACACCGGCCTGAATATTCTCAACACCGC
CGACGTGAACATTTCCACTGCCGAAGACCCGGTGGAGATCAACCTTGAGGGCATCAACCAGGTCAACGTCAACCCGAAAC
AGGGCATGGACTTCTCCCAGGCCTTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGTGAGATTCGTGAC
CTGGAAACCGCGGAAATCGCCATCAAGGCCGCGCAGACTGGGCATATGGTCATGTCCACCCTGCACACCAACAGCGCCGC
CGAAACGCTGACGCGCTTGCGCAATATGGGCGTGCCGGCTTTCAACATCGCCACCTCGGTCAACCTGATCATCGCTCAGC
GCCTGGCGCGCAAGCTGTGCCCGCACTGCAAGAAGGAAGTGCAGATCCCCCACGAAACCCTGCTGCACGAAGGCTTCCCC
GAAGACAAGATCGGCACCTTCAAGATTTACGCCCCAGTCGGCTGCGAGAACTGCAAGGGCGGCTATAAAGGCCGCTTGGG
TATTTATGAAGTGGTTAAAAACACGCCGGCCCTGCAGCGGATTATCATGGAGGACGGCAACTCCATCGATATTGGCGTAC
AGATGCGTAAAGATGGTTTCAACGACTTGCGTACATCCGGCCTGGAGAAAGCCATGCAGGGCATCACCAGCCTTGCAGAG
GTTAACCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.474

100

0.598
  pilB Acinetobacter baylyi ADP1

58.201

100

0.582
  pilB Legionella pneumophila strain ERS1305867

57.168

99.647

0.57
  pilB Vibrio parahaemolyticus RIMD 2210633

51.287

95.944

0.492
  pilB Vibrio cholerae strain A1552

50.181

97.707

0.49
  pilF Neisseria gonorrhoeae MS11

48.673

99.647

0.485
  pilB Vibrio campbellii strain DS40M4

50.092

95.414

0.478
  pilF Thermus thermophilus HB27

37.876

99.647

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.925

93.651

0.374