Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   NHB23_RS09725 Genome accession   NZ_CP102716
Coordinates   2115150..2115587 (-) Length   145 a.a.
NCBI ID   WP_049179754.1    Uniprot ID   A0A853J726
Organism   Lacticaseibacillus rhamnosus strain DM065     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2081676..2123001 2115150..2115587 within 0


Gene organization within MGE regions


Location: 2081676..2123001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHB23_RS09510 (NHB23_009510) - 2081676..2082848 (-) 1173 WP_049179690.1 GH25 family lysozyme -
  NHB23_RS09515 (NHB23_009515) - 2082859..2083308 (-) 450 WP_049179692.1 phage holin -
  NHB23_RS09520 (NHB23_009520) - 2083323..2083622 (-) 300 WP_308706291.1 hypothetical protein -
  NHB23_RS09525 (NHB23_009525) - 2083654..2083797 (-) 144 WP_005688592.1 XkdX family protein -
  NHB23_RS09530 (NHB23_009530) - 2083794..2084123 (-) 330 WP_049179694.1 hypothetical protein -
  NHB23_RS09535 (NHB23_009535) - 2084139..2087093 (-) 2955 WP_049179695.1 phage tail protein -
  NHB23_RS09540 (NHB23_009540) - 2087094..2089007 (-) 1914 WP_049179698.1 distal tail protein Dit -
  NHB23_RS09545 (NHB23_009545) - 2089008..2093870 (-) 4863 WP_049179700.1 phage tail tape measure protein -
  NHB23_RS09550 (NHB23_009550) gpG 2093993..2094406 (-) 414 WP_049179703.1 phage tail assembly chaperone G -
  NHB23_RS09555 (NHB23_009555) - 2094477..2094662 (-) 186 WP_049179705.1 Ig-like domain-containing protein -
  NHB23_RS09560 (NHB23_009560) - 2094719..2095339 (-) 621 WP_049179707.1 major tail protein -
  NHB23_RS09565 (NHB23_009565) - 2095373..2095759 (-) 387 WP_016389063.1 hypothetical protein -
  NHB23_RS09570 (NHB23_009570) - 2095759..2096145 (-) 387 WP_049179710.1 HK97-gp10 family putative phage morphogenesis protein -
  NHB23_RS09575 (NHB23_009575) - 2096145..2096474 (-) 330 WP_049179711.1 head-tail adaptor protein -
  NHB23_RS09580 (NHB23_009580) - 2096464..2096823 (-) 360 WP_049179713.1 head-tail connector protein -
  NHB23_RS09585 (NHB23_009585) - 2096834..2097073 (-) 240 WP_049179716.1 Ig-like domain-containing protein -
  NHB23_RS09590 (NHB23_009590) - 2097091..2098293 (-) 1203 WP_049179718.1 phage major capsid protein -
  NHB23_RS09595 (NHB23_009595) - 2098335..2098964 (-) 630 WP_049179720.1 HK97 family phage prohead protease -
  NHB23_RS09600 (NHB23_009600) - 2098918..2100171 (-) 1254 WP_049179722.1 phage portal protein -
  NHB23_RS09605 (NHB23_009605) - 2100177..2100368 (-) 192 WP_003661399.1 hypothetical protein -
  NHB23_RS09610 (NHB23_009610) - 2100380..2102092 (-) 1713 WP_032953807.1 terminase large subunit -
  NHB23_RS09615 (NHB23_009615) - 2102114..2102569 (-) 456 WP_005686963.1 P27 family phage terminase small subunit -
  NHB23_RS09620 (NHB23_009620) - 2102770..2103564 (-) 795 WP_049179724.1 HNH endonuclease -
  NHB23_RS09625 (NHB23_009625) - 2103767..2104090 (-) 324 WP_049179727.1 ribonucleoside-diphosphate reductase -
  NHB23_RS09630 (NHB23_009630) - 2104087..2104632 (-) 546 WP_049179729.1 NUMOD4 domain-containing protein -
  NHB23_RS09635 (NHB23_009635) - 2104625..2105773 (-) 1149 WP_049179731.1 GcrA family cell cycle regulator -
  NHB23_RS09640 (NHB23_009640) - 2106314..2107174 (-) 861 WP_005686948.1 hypothetical protein -
  NHB23_RS09645 (NHB23_009645) - 2107462..2107905 (-) 444 WP_005714759.1 ArpU family phage packaging/lysis transcriptional regulator -
  NHB23_RS09650 (NHB23_009650) - 2108347..2108529 (-) 183 WP_048488379.1 hypothetical protein -
  NHB23_RS09655 (NHB23_009655) - 2108579..2108818 (-) 240 WP_014569479.1 hypothetical protein -
  NHB23_RS09660 (NHB23_009660) - 2108863..2109051 (-) 189 WP_005688546.1 hypothetical protein -
  NHB23_RS09665 (NHB23_009665) - 2109048..2109326 (-) 279 WP_373418399.1 hypothetical protein -
  NHB23_RS09670 (NHB23_009670) - 2109355..2109531 (-) 177 WP_175265236.1 hypothetical protein -
  NHB23_RS09675 (NHB23_009675) - 2109521..2110051 (-) 531 WP_049179739.1 DUF1642 domain-containing protein -
  NHB23_RS09680 (NHB23_009680) - 2110048..2110230 (-) 183 WP_048488361.1 hypothetical protein -
  NHB23_RS09685 (NHB23_009685) - 2110227..2110934 (-) 708 WP_049179741.1 N-6 DNA methylase -
  NHB23_RS09690 (NHB23_009690) - 2110927..2111778 (-) 852 WP_032964565.1 DNA cytosine methyltransferase -
  NHB23_RS09695 (NHB23_009695) - 2111792..2112157 (-) 366 WP_016364507.1 hypothetical protein -
  NHB23_RS09700 (NHB23_009700) - 2112154..2112408 (-) 255 WP_049179744.1 hypothetical protein -
  NHB23_RS09705 (NHB23_009705) - 2112395..2112682 (-) 288 WP_049179746.1 hypothetical protein -
  NHB23_RS09710 (NHB23_009710) - 2112695..2113039 (-) 345 WP_049179748.1 hypothetical protein -
  NHB23_RS09715 (NHB23_009715) - 2113041..2114303 (-) 1263 WP_049179750.1 DnaB-like helicase C-terminal domain-containing protein -
  NHB23_RS09720 (NHB23_009720) - 2114300..2115133 (-) 834 WP_049179752.1 helix-turn-helix domain-containing protein -
  NHB23_RS09725 (NHB23_009725) ssb 2115150..2115587 (-) 438 WP_049179754.1 single-stranded DNA-binding protein Machinery gene
  NHB23_RS09730 (NHB23_009730) - 2115602..2116270 (-) 669 WP_080980177.1 putative HNHc nuclease -
  NHB23_RS09735 (NHB23_009735) - 2116273..2117028 (-) 756 WP_049179756.1 ERF family protein -
  NHB23_RS09740 (NHB23_009740) - 2117028..2117915 (-) 888 WP_049179759.1 DUF1351 domain-containing protein -
  NHB23_RS09745 (NHB23_009745) - 2117933..2118136 (-) 204 WP_049179761.1 hypothetical protein -
  NHB23_RS09750 (NHB23_009750) - 2118378..2118734 (-) 357 WP_049179763.1 DUF771 domain-containing protein -
  NHB23_RS09755 (NHB23_009755) - 2118813..2118962 (-) 150 WP_180530720.1 hypothetical protein -
  NHB23_RS09760 (NHB23_009760) - 2118959..2119777 (-) 819 WP_049179765.1 ORF6N domain-containing protein -
  NHB23_RS09765 (NHB23_009765) - 2119802..2120029 (-) 228 WP_049179767.1 hypothetical protein -
  NHB23_RS09770 (NHB23_009770) - 2120193..2120969 (+) 777 WP_049179769.1 XRE family transcriptional regulator -
  NHB23_RS09775 (NHB23_009775) - 2121024..2121701 (+) 678 WP_049179771.1 tcdA-E operon negative regulator -
  NHB23_RS09780 (NHB23_009780) - 2121874..2123001 (+) 1128 WP_049179772.1 site-specific integrase -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 15906.53 Da        Isoelectric Point: 5.7223

>NTDB_id=718543 NHB23_RS09725 WP_049179754.1 2115150..2115587(-) (ssb) [Lacticaseibacillus rhamnosus strain DM065]
MLNSVALTGRLTKPVDLRYTQSGTAVGSFTIAVDRQFRSANGERETDFINCAIWRKSAENFANFTHKGSLVGIEGHIQTR
TYDNAQGQKVFVTEVIVENFALLEPRQASQDGQQRSANNPAAASQGNSFANNGQPVDIRDDDLPF

Nucleotide


Download         Length: 438 bp        

>NTDB_id=718543 NHB23_RS09725 WP_049179754.1 2115150..2115587(-) (ssb) [Lacticaseibacillus rhamnosus strain DM065]
ATGCTTAATTCAGTTGCTTTAACAGGCAGATTAACTAAACCGGTTGACCTTCGCTACACACAAAGCGGAACGGCAGTTGG
TTCATTTACGATTGCTGTTGATCGCCAATTTCGCAGCGCAAACGGCGAACGTGAAACTGACTTCATCAATTGTGCCATCT
GGCGTAAGTCTGCTGAGAACTTTGCCAACTTCACGCACAAGGGTTCACTTGTTGGCATCGAAGGCCATATCCAAACGCGT
ACGTATGATAACGCGCAAGGGCAGAAAGTATTCGTGACCGAGGTAATCGTTGAGAATTTTGCTTTGCTTGAGCCACGGCA
AGCGTCTCAGGATGGTCAACAACGATCGGCTAATAACCCAGCGGCCGCAAGCCAAGGCAACAGTTTTGCCAACAATGGTC
AGCCAGTCGATATCAGGGATGATGATCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A853J726

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.662

  ssbA Bacillus subtilis subsp. subtilis str. 168

47.674

100

0.566

  ssb Neisseria gonorrhoeae MS11

32.37

100

0.386

  ssbB Bacillus subtilis subsp. subtilis str. 168

52.83

73.103

0.386

  ssb Neisseria meningitidis MC58

31.792

100

0.379

  ssbB Streptococcus sobrinus strain NIDR 6715-7

37.241

100

0.372

  ssb Vibrio cholerae strain A1552

30.636

100

0.366