Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NT239_RS06890 Genome accession   NZ_CP102611
Coordinates   1510712..1512418 (+) Length   568 a.a.
NCBI ID   WP_373976470.1    Uniprot ID   -
Organism   Chitinibacter sp. SCUT-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1505712..1517418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NT239_RS06860 (NT239_06860) rplS 1506104..1506478 (-) 375 WP_373976466.1 50S ribosomal protein L19 -
  NT239_RS06865 (NT239_06865) trmD 1506542..1507294 (-) 753 WP_373976690.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  NT239_RS06870 (NT239_06870) rimM 1507344..1507889 (-) 546 WP_373976467.1 ribosome maturation factor RimM -
  NT239_RS06875 (NT239_06875) rpsP 1507900..1508145 (-) 246 WP_348946004.1 30S ribosomal protein S16 -
  NT239_RS06880 (NT239_06880) ffh 1508245..1509588 (-) 1344 WP_373976468.1 signal recognition particle protein -
  NT239_RS06885 (NT239_06885) - 1509670..1510476 (+) 807 WP_373976469.1 inner membrane protein YpjD -
  NT239_RS06890 (NT239_06890) pilF 1510712..1512418 (+) 1707 WP_373976470.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NT239_RS06895 (NT239_06895) pilC 1512436..1513659 (+) 1224 WP_373976471.1 type II secretion system F family protein Machinery gene
  NT239_RS06900 (NT239_06900) pilD 1513659..1514519 (+) 861 WP_373976472.1 A24 family peptidase Machinery gene
  NT239_RS06905 (NT239_06905) coaE 1514523..1515149 (+) 627 WP_373976473.1 dephospho-CoA kinase -
  NT239_RS06910 (NT239_06910) zapD 1515175..1515933 (+) 759 WP_373976474.1 cell division protein ZapD -
  NT239_RS06915 (NT239_06915) - 1515930..1516127 (+) 198 WP_373976475.1 DNA gyrase inhibitor YacG -
  NT239_RS06920 (NT239_06920) - 1516320..1517069 (+) 750 WP_373976476.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62578.09 Da        Isoelectric Point: 5.4364

>NTDB_id=718143 NT239_RS06890 WP_373976470.1 1510712..1512418(+) (pilF) [Chitinibacter sp. SCUT-21]
MSTPQISGLARLLVQHGRLSETDAEALQSAAISNKSTFIEQLTQSKKLTAREVAEFSAQTFGYPLLDLDRIDSSYLPQGV
LDNNVMRTQRLVPLFKRGTKLFIGLSDITNLEAVEKVRFQTNSQVEPIIVEDNKLIILLNKTIEATGANLKDMVIDEADL
ALDGGEQEPTAAETQMGQEIDDAPVVKYLQKMLMDAINSGASDIHFEPFEKFYRIRFRIDGVLREVAQPPLAIKEKISSR
IKVISKLDISEKRIPQDGRMKLVLSKSRAIDFRVSTLPTMHGEKIVMRILDPSSATLGIDALGYDPDQKALILDAIQRPY
GMVLVTGPTGSGKTVSLYTCLNLLNKPDINISTAEDPAEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRD
LETADIAIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIQPFNIASSVILITAQRLARRLCSCKKHVDIPAQALLDAGFQQ
EELDDTWQPYGPVGCELCKNTGYKGRVGIYQVMPISDEINRLIMNHGNAIDIADQARKEGVLSLREAGLRKVRQGLTSLE
EIMAVTNV

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=718143 NT239_RS06890 WP_373976470.1 1510712..1512418(+) (pilF) [Chitinibacter sp. SCUT-21]
ATGAGCACCCCGCAAATTTCTGGCTTAGCTCGCTTATTAGTACAGCACGGACGCCTAAGTGAGACCGACGCAGAAGCCCT
GCAATCGGCGGCCATAAGCAACAAAAGCACTTTTATTGAGCAGCTAACTCAAAGTAAAAAACTCACTGCACGCGAGGTTG
CTGAGTTTTCAGCACAGACTTTTGGCTATCCGCTACTAGACCTCGATCGAATTGATTCAAGCTACCTCCCGCAAGGCGTT
CTTGATAACAATGTAATGCGCACACAGCGCCTTGTTCCGCTATTCAAGCGCGGCACAAAACTCTTTATTGGCCTCTCTGA
CATTACCAATCTTGAGGCAGTAGAAAAGGTTCGCTTCCAAACCAACTCACAAGTTGAGCCAATTATTGTCGAAGACAATA
AGTTGATTATTTTGCTCAACAAAACCATCGAAGCTACCGGCGCAAATCTGAAAGACATGGTAATTGACGAGGCAGATTTA
GCGCTCGATGGTGGAGAACAAGAACCAACAGCAGCTGAAACCCAAATGGGGCAGGAGATTGATGATGCGCCAGTGGTTAA
GTACCTGCAAAAAATGCTAATGGATGCCATCAATAGTGGCGCCTCCGACATTCATTTTGAGCCCTTTGAAAAATTCTATC
GAATTAGATTTCGCATTGATGGCGTTTTACGTGAGGTTGCACAACCCCCATTAGCCATTAAGGAAAAAATCTCCTCCAGG
ATCAAGGTAATCTCTAAGCTCGACATTTCAGAAAAACGAATCCCACAAGATGGACGGATGAAGTTAGTTTTATCCAAGTC
TCGTGCAATTGATTTCCGTGTTTCGACATTGCCGACCATGCATGGCGAAAAAATCGTAATGCGGATCTTGGACCCCTCTT
CTGCCACGCTCGGGATCGATGCGCTCGGCTATGATCCCGATCAAAAAGCACTGATTTTAGATGCCATCCAGCGTCCATAT
GGCATGGTACTAGTGACCGGGCCCACCGGCTCAGGCAAAACCGTGTCACTTTACACCTGCCTCAATTTACTGAATAAGCC
AGACATTAACATCTCGACAGCAGAAGACCCTGCTGAGATTAACTTGCCAGGCATCAATCAAGTCAATGTAAATGATAAAG
CTGGGCTAACCTTTTCTGCCGCGCTAAAAGCGTTCCTGCGCCAAGACCCCGACATCATTATGGTCGGTGAGATTCGCGAC
CTTGAAACAGCGGACATTGCAATTAAAGCAGCCCAGACTGGCCACATGGTGTTCTCAACGCTCCACACTAACGACGCACC
AACTACGTTAACGCGGATGTTGAATATGGGCATCCAGCCATTCAATATTGCTTCCTCCGTAATACTGATTACGGCACAAC
GGCTTGCACGACGCCTATGCAGTTGCAAAAAACATGTCGATATTCCGGCGCAAGCACTGCTAGATGCAGGCTTTCAGCAA
GAAGAACTAGACGACACCTGGCAACCCTATGGGCCAGTGGGTTGTGAACTATGCAAAAACACAGGCTATAAAGGTCGAGT
TGGCATTTATCAAGTCATGCCGATTAGCGATGAAATTAATCGTCTCATCATGAATCATGGGAATGCCATTGATATTGCCG
ACCAAGCCCGAAAAGAAGGCGTACTCAGCCTACGTGAGGCAGGACTAAGAAAAGTACGTCAAGGACTCACCTCCCTCGAA
GAAATCATGGCTGTAACAAACGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.93

100

0.549
  pilB Acinetobacter baumannii D1279779

54.737

100

0.549
  pilB Acinetobacter baylyi ADP1

53.765

100

0.54
  pilB Legionella pneumophila strain ERS1305867

51.228

100

0.514
  pilB Vibrio cholerae strain A1552

46.643

99.648

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

99.296

0.452
  pilB Vibrio campbellii strain DS40M4

44.858

99.296

0.445
  pilF Thermus thermophilus HB27

37.828

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.513

94.014

0.371