Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NUU08_RS11675 Genome accession   NZ_CP102522
Coordinates   2263557..2264630 (-) Length   357 a.a.
NCBI ID   WP_014570824.1    Uniprot ID   -
Organism   Lactococcus lactis strain R-707-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2258557..2269630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUU08_RS11630 (NUU08_11625) - 2258748..2258870 (-) 123 WP_023164646.1 hypothetical protein -
  NUU08_RS11635 (NUU08_11630) - 2258867..2259049 (-) 183 WP_003130605.1 hypothetical protein -
  NUU08_RS11640 (NUU08_11635) - 2259065..2259754 (-) 690 WP_058221713.1 Rha family transcriptional regulator -
  NUU08_RS11645 (NUU08_11640) - 2259813..2260046 (-) 234 WP_014570819.1 helix-turn-helix transcriptional regulator -
  NUU08_RS11650 (NUU08_11645) - 2260223..2260633 (+) 411 WP_014570820.1 helix-turn-helix domain-containing protein -
  NUU08_RS11655 (NUU08_11650) - 2260644..2261228 (+) 585 WP_058221714.1 hypothetical protein -
  NUU08_RS11660 (NUU08_11655) - 2261284..2261823 (+) 540 WP_023189015.1 PH domain-containing protein -
  NUU08_RS11665 (NUU08_11660) - 2261949..2263406 (+) 1458 WP_058221720.1 recombinase family protein -
  NUU08_RS11670 (NUU08_11665) - 2263403..2263543 (-) 141 WP_023164653.1 hypothetical protein -
  NUU08_RS11675 (NUU08_11670) comGB 2263557..2264630 (-) 1074 WP_014570824.1 competence type IV pilus assembly protein ComGB Machinery gene
  NUU08_RS11680 (NUU08_11675) comGA 2264524..2265463 (-) 940 Protein_2276 competence type IV pilus ATPase ComGA -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40366.59 Da        Isoelectric Point: 10.4062

>NTDB_id=717466 NUU08_RS11675 WP_014570824.1 2263557..2264630(-) (comGB) [Lactococcus lactis strain R-707-1]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKKFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=717466 NUU08_RS11675 WP_014570824.1 2263557..2264630(-) (comGB) [Lactococcus lactis strain R-707-1]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGGCTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAAAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACCTTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

82.698

95.518

0.79
  comYB Streptococcus gordonii str. Challis substr. CH1

53.666

95.518

0.513
  comYB Streptococcus mutans UA140

53.372

95.518

0.51
  comYB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae D39

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae R6

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae Rx1

51.343

93.838

0.482
  comGB/cglB Streptococcus mitis SK321

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.149

93.838

0.471