Detailed information NTG0717452

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NUU08_RS09345
• Genome accession: NZ_CP102522
• Coordinates: 1853251..1853898 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis strain R-707-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1848251..1858898

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NUU08_RS09320 (NUU08_09310)	atpF	1848322..1848828 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
NUU08_RS09325 (NUU08_09315)	atpB	1848843..1849556 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
NUU08_RS09330 (NUU08_09320)	-	1849601..1849816 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
NUU08_RS09335 (NUU08_09325)	-	1850000..1850776 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
NUU08_RS09340 (NUU08_09330)	-	1851061..1853270 (-)	2210	Protein_1821	DNA internalization-related competence protein ComEC/Rec2	-
NUU08_RS09345 (NUU08_09335)	comEA	1853251..1853898 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
NUU08_RS09350 (NUU08_09340)	-	1853958..1855319 (-)	1362	WP_058221762.1	ABC transporter permease	-
NUU08_RS09355 (NUU08_09345)	-	1855316..1856248 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
NUU08_RS09360 (NUU08_09350)	-	1856353..1856751 (-)	399	WP_004255236.1	hypothetical protein	-
NUU08_RS09365 (NUU08_09355)	-	1856855..1857418 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
NUU08_RS09370 (NUU08_09360)	-	1857591..1858769 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=717452 NUU08_RS09345 WP_004255240.1 1853251..1853898(-) (comEA) [Lactococcus lactis strain R-707-1]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=717452 NUU08_RS09345 WP_004255240.1 1853251..1853898(-) (comEA) [Lactococcus lactis strain R-707-1]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367