Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NQ186_RS03090 Genome accession   NZ_CP102358
Coordinates   698279..700357 (+) Length   692 a.a.
NCBI ID   WP_257356864.1    Uniprot ID   -
Organism   Pseudomonas zeae strain BIM B-582     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 693279..705357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ186_RS03070 (NQ186_03070) pilM 695491..696555 (+) 1065 WP_093430076.1 pilus assembly protein PilM Machinery gene
  NQ186_RS03075 (NQ186_03075) - 696555..697121 (+) 567 WP_257356862.1 PilN domain-containing protein -
  NQ186_RS03080 (NQ186_03080) pilO 697118..697741 (+) 624 WP_099757776.1 type 4a pilus biogenesis protein PilO -
  NQ186_RS03085 (NQ186_03085) - 697738..698265 (+) 528 WP_257356863.1 pilus assembly protein PilP -
  NQ186_RS03090 (NQ186_03090) pilQ 698279..700357 (+) 2079 WP_257356864.1 type IV pilus secretin PilQ Machinery gene
  NQ186_RS03095 (NQ186_03095) aroK 700362..700880 (+) 519 WP_011332081.1 shikimate kinase AroK -
  NQ186_RS03100 (NQ186_03100) aroB 701033..702133 (+) 1101 WP_093430081.1 3-dehydroquinate synthase -
  NQ186_RS03105 (NQ186_03105) - 702144..703733 (+) 1590 WP_099757779.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75080.64 Da        Isoelectric Point: 5.8300

>NTDB_id=716395 NQ186_RS03090 WP_257356864.1 698279..700357(+) (pilQ) [Pseudomonas zeae strain BIM B-582]
MNRIFSTLGFSLWIALMSPMVLAANLRTLDVAALPGDRVELKLSFDGPPPQPKVYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKERTRLIVSLTQLAPYTTRVEGNNLFVVVGQGAPAAASRPAAVAPRTTAKAAAPAKAFVPKSRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILTFARTQLPDKLRVRLDVKDFATPVQFVNAGVTADRTVITVEPSG
TYEYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVN
GSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=716395 NQ186_RS03090 WP_257356864.1 698279..700357(+) (pilQ) [Pseudomonas zeae strain BIM B-582]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAG
GACGCTGGACGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCGCCACCACAACCCAAGG
TCTACACCACCGAATCGCCGGCGCGGATTGCCCTGGACCTGCCCGGTGTCGCCAGTCAGTTGGCCAGCAAGAACCTCGAT
CTGGGCAGTGGCAATGCGCGCACCGCTACTGTGGTTGAGGCCAAGGAACGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCGTACACCACGCGGGTTGAGGGCAATAACCTGTTCGTGGTGGTCGGTCAGGGCGCACCGGCAGCTGCGTCGCGCC
CTGCAGCCGTTGCGCCCCGCACTACGGCTAAAGCGGCCGCACCCGCCAAGGCCTTTGTGCCGAAAAGCCGGGCGATTCGC
GGTGTGGACTTCCAGCGTGGTACGGCGGGGGAAGGCAATGTGGTCATCGACCTGTCCGACCCGACCATCGCCCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCACCTTCGCGCGCACGCAGTTGCCGGACAAGTTGCGGGTACGCCTCGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGAGTAACCGCTGATCGCACAGTCATTACCGTTGAACCCAGTGGC
ACCTACGAGTACTCCACCTTCCAGACCGACAACAAACTGACCGTCAGCATCCGCCCGATGACCGTCGATGACCTGCAAAA
ACGCAACGCTGACCGTCAGGCGTACGTCGGCGAAAAGCTCTCGCTGAACTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAATCTGGTTGCCAGCGACACGGTGCAGGGTGGCATCACCTTGCGCCTGCAAAAT
GTGCCGTGGGATCAGGCCCTGGATCTGGTGTTGAAAACCAAGGGCCTGGATAAGCGCAAGATCGGCAATGTGCTGCTGGT
GGCGCCGGCCGATGAAATCGCTGCCCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAACTGGCGCCGTTGC
GCCGCGAGCTGCTACAGGTGAACTACGCCAAAGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCTGAGGCG
AAAATCGACGAGCGCGGTTCGATTACCGTCGACGAGCGCACCAACAATATCATTGCCTATCAGACTCAGGATCGCCTCGA
CGAACTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAAGCGCGGATTGTCGAGGCCAACG
TCGATTACGACAAAAGTCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACGCTTCCGGGGTCAAC
GGTTCATCGACCACCATTGGTACGCCGGGCAGTACCAGCACCAATTCGCCGTTCGTCGACATGGGCACTGTGGGTAATAC
ATCGGGGATCGGCATTGCCTTCATTACCGACAATGTCCTGCTTGATCTTGAGCTGACCGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCCGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCGTAT
CAGGAAGCCAGTTCCAGCGGCGCGACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACCCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAATGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
ATCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.184

100

0.757
  comQ Acinetobacter baylyi ADP1

41.643

100

0.418
  pilQ Acinetobacter baumannii D1279779

38.462

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.462

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

37.87

97.688

0.37
  pilQ Legionella pneumophila strain Lp02

37.87

97.688

0.37