Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NQ186_RS03070 Genome accession   NZ_CP102358
Coordinates   695491..696555 (+) Length   354 a.a.
NCBI ID   WP_093430076.1    Uniprot ID   -
Organism   Pseudomonas zeae strain BIM B-582     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 690491..701555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ186_RS03060 (NQ186_03060) - 691238..692506 (+) 1269 WP_090280412.1 malic enzyme-like NAD(P)-binding protein -
  NQ186_RS03065 (NQ186_03065) - 692827..695271 (-) 2445 WP_371748300.1 penicillin-binding protein 1A -
  NQ186_RS03070 (NQ186_03070) pilM 695491..696555 (+) 1065 WP_093430076.1 pilus assembly protein PilM Machinery gene
  NQ186_RS03075 (NQ186_03075) - 696555..697121 (+) 567 WP_257356862.1 PilN domain-containing protein -
  NQ186_RS03080 (NQ186_03080) pilO 697118..697741 (+) 624 WP_099757776.1 type 4a pilus biogenesis protein PilO -
  NQ186_RS03085 (NQ186_03085) - 697738..698265 (+) 528 WP_257356863.1 pilus assembly protein PilP -
  NQ186_RS03090 (NQ186_03090) pilQ 698279..700357 (+) 2079 WP_257356864.1 type IV pilus secretin PilQ Machinery gene
  NQ186_RS03095 (NQ186_03095) aroK 700362..700880 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38107.72 Da        Isoelectric Point: 4.8109

>NTDB_id=716394 NQ186_RS03070 WP_093430076.1 695491..696555(+) (pilM) [Pseudomonas zeae strain BIM B-582]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRLLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLSTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=716394 NQ186_RS03070 WP_093430076.1 695491..696555(+) (pilM) [Pseudomonas zeae strain BIM B-582]
GTGCTGGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGTTCCACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTATGCGGTAGAGCCATTGCCAGTCAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTTGAAGGCGTCGGTCAGGCCCTGAGCCGTTTACTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCAGTC
GCAGTGGCCGGTTCCGCCGTGATCACCAAGATCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAAGATCGAGGCCGATCAGTACATTCCCTACCCGCTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTGTCACCGC
GCAACCCTGAGCGGGTCAACGTGTTGCTCGCTGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTGGCTCTG
GCCGGGCTGACCGCACGGGTAGTCGACGTCGAAGCCTATGCGCTGGAGCGCTCGTTCGGCTTGCTGGCCACGCAACTGGC
GGCGTCTCAGGAGCGTCTGACTGTCGCTGTTGTCGACATCGGCGCGACCATGACCACGCTCAGCGTCCTGCACAATGGCA
AGATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGGCAACTGACCGAGGAAATCCAGCGTCGCTATGGCCTGACCCTC
GAACAAGCGGGGCTGGCGAAAAAGCAGGGCGGTCTGCCCGATGATTACATCAGCGAGGTCTTGCAACCGTTTCGCGAGGC
GCTGGTGCAGCAAGTGTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGGCAATACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACGGCTTCAGTGCCCGGACTGGATCGGCTGATCGAGCAGCGCCTGAGCACACCGACACAAGTAGCCAACCCG
TTTGCCAACATGGCCCTGAGCAGCAAAGTCAACGCCGGGGCTTTGGCCAGCGACGCGCCGGCGCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475